重组人磷脂酰肌醇3激酶(Human PI3Kb)

PI3Kb Human
Phosphoinositide 3-kinase beta p110β/p85α Human Recombinant
Catalog No. PKA-333

Synonyms:
Phosphoinositide 3-kinase beta p110β/p85α, PI3K β, PI3Kb.

Introduction:
The PI3Kb isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation of
PI3Kb is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2).
It was also shown that PI3Kb is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr,
HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis.
PI3Kb is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line.
It was shown by several research groups that the catalytic subunit of PI3Kb can be activated by Gbg subunits of G-protein coupled receptors.

Description:
Phosphoinositide 3-kinase beta Human Recombinant is a glycosilated protein having a molecular weight as follows: p85α chain 83.5 kDa, p110β chain 124.3 kDa.

Source:
Sf9 insect cells.

Physical Appearance:
Sterile filtered liquid formualtion.

Formulation:
0.5 mg/ml solution in 10mM Hepes, pH 7.5, 100mM NaCl, 2.5mM MgCl2, and 50% glycerol.

Stability:
PI3Kb although stable at 4°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 90.0% as determined by SDS Page.

Biological Activity:
~ 3 nmol/mg/min using phosphatidylinositol as the substrate.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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