重组人磷脂酰肌醇3激酶(Human PI3Kd GST)

PI3Kd Human
Phosphoinositide 3-kinase p110d/p85a Human Recombinant
Catalog No. PKA-330

Synonyms:
Phosphoinositide 3-kinase p110d/p85a, PI3Kd.

Description:
The class IA PI3Kδ is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3Kδ in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgE induced degranulation and cytokine release. In neutrophils a role for PI3Kδ in TNFa-induced signaling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the d-specific inhibitor IC87114. The catalytic subunit carries a GST-Tag which facilitates the protein’s application in typical GST pull-down assays and the heterodimer was purified by affinity chromatography. The molecular weight for p110δ chain is 120.2 kDa (without Tag) and the p85α chain 83.5 kDa.

Source:
Sf9 insect cells.

Physical Appearance:
Sterile filtered liquid formualtion.

Formulation:
PI3Kd solution in 25mM Tris-HCl, pH 8.0, 50mM NaCl, 0.5mM MgCl2, and 50% glycerol.

Stability:
PI3Kd although stable at 4°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by SDS Page.

Activity:
3,000 U/mg.

Unit Definition:
1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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