重组人细胞周期蛋白依赖性激酶4(Human CDK4)

CDK-4 Human
Cyclin-Dependent Kinase 4 Human Recombinant
Catalog No. PKA-325

Synonyms:
Cell division protein kinase 4, CDK4, EC 2.7.11.22, Cyclin-dependent kinase 4, PSK-J3, CMM3,CDK-4, MGC14458.

Introduction:
Cyclin-dependent kinase 4 combines with the protein cyclin D to form a protein complex that promotes the passage of cells through the G1 checkpoint in the cell growth cycle. This cellular activity is regulated by the protein p16 produced by p16INK4a. Normally, p16 controls cell growth by inhibiting the activity of the CDK4–cyclin D complex and stopping cells at the G1 checkpoint.

Description:
Cyclin-Dependent Kinase 4 Human Recombinant is expressed in E. coli as a full-length protein having a molecular weight of 38 kDa fused to an amino terminal hexahistidine tag.
The CDK4 is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Formulation:
CDK4 is supplied in 10mM Tris-HCl (pH 8), 250mM NaCl, 0.5mM DTT, 1.5mM Cysteine, and 50% Glycerol.

Application:
CDK-4 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments.
The biological activity of this product has not yet been tested.

Storage Conditions:
Store at 4°C if entire vial will be used within 1-2 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 95% as determined by SDS-PAGE.
Single band on Western Blot

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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