人尿激酶(Human Urokinase)

Urokinase Human
Urokinase Human
Catalog No. ENZ-264

Synonyms:
Urokinase, Abbokinase, Urokinase-type Plasminogen Activator,uPA, EC 3.4.21.73, UK.

Introduction:
Urokinase (UK) is a serine protease, which is one of biological plasminogen activators.
It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing.
It can be obtained from human urine or kidney cell culture.

Description:
Urokinase is a two-chain glycoprotein containing 411 amino acids with 12 dissulfide bonds. Its molecular weight is 54,000 Dalton.

Source:
Human urine

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The Urokinase was lyophilized from a concentrated (1mg/ml) solution containing phosphate buffer.

Solubility:
It is recommended to reconstitute the lyophilized Urokinase in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Urokinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Urokinase should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 90.0% as determined by SDS-PAGE.

Specific Activity:
77,000IU/mg powder, 168,000IU/mg protein.

Activity:
1nM UK will cause a change in absorbance of 0.001 at 405nm in 1 minute at R/T in 100 ul 0.05M Tris-HCl, 0.1M NaCl, pH 7.4, using S2444 (0.6mM) as the substrate.

Contaminents:
Free of: Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.