Avidin Biotin
Modified Affinity Purified Avidin
Catalog No. PRO-446
Synonyms:
Avidin, AVD, AVID.
Description:
Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons.
Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii.
Advantages:
1. Avidin-Biotin does not exhibit unwanted non-specific binding that have been previously reported for the native egg white avidin.
2. The non-specific properties of Avidin-Biotin have been tested in ELISA, Western Blot & Immunohistochemistry techniques and have been found to be superior to unmodified avidin.
3. Avidin-Biotin rarely affected by pH values between the range of pH-2 to pH-12 or by guanidine-HCl concentrations up to 8M at neutral pH.
Applications:
Avidin-Biotin may be used in combination with biotin in various techniques such as ELISA, Western blotting, immunohistochemistry etc…
Physical Appearance:
Lyophilized freeze dry Powder.
Formulation:
Salt-free lyophilized powder with no preservative added.
Solubility:
We highly recommended to prepare a 1 mg/ml solution; Reconstitute using cold deionized pyrogen free water and mix gently until powder is completely dissolved.
Stability:
Store protein solution at 2-8°C.
Activity:
Binding activity of Avidin-Biotin is 10-15units/mg protein.
One unit of Avidin activity is defined as the amount of protein which will bind 1µg d-biotin.
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» Avidin
更多
更多
企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海希美化学有限公司
公司地址
上海市松江区陆家圈路168弄113号
客服电话