Catalogue Number | ANT-345 | ||
Type | Mouse Antibody Monoclonal. | ||
Introduction | Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH. | ||
Clone | P1C2AT. | ||
Immunogen | Anti Streptavidin mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant Streptavidin amino acids 25-183 purified from E. coli. | ||
Ig Subclass | Mouse IgG2b heavy chain and κ light chain. | ||
Purification Method | Streptavidin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. | ||
Protein formulation | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. | ||
Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. | ||
Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. | ||
Applications | Streptavidin antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~3,000. Recommended starting dilution is 1:2,000. | ||
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||
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企业名称
上海希美化学有限公司
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信用代码
310228001203103
成立日期
2009-05-31
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10
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化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
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