HORIBA高灵敏一体式FluoroMax-4荧光光谱仪

Fluorescence spectroscopy is an analytical technique with high sensitivity and selectivity. By characterizing the components in the EV-coat solution with fluorescence, a detailed analytical profile can be formed for each component. This can help trace the source of errors in the EV-coat solution, and contribute to selection of the best formulation. Quality-control tests can be performed periodically to check for consistent bath composition.

马萨诸塞大学韩刚博士就在这一方面做出了突破，他成功 制备出一种新的近红外光敏剂。 这种光敏剂能够穿透深层 组织，不仅能解决光动力学疗法穿透能力差的问题，同时 还能提高光敏剂的反应效率 （在近红外范围表现出显著增 强的吸收和单态氧生成效率）。这一突破不仅是首创性的， 且具有非常重要的意义，有望 将光动力学疗法推向深层组 织和大尺寸的癌症治疗 。

四川大学吴鹏教授团队与加拿大 Waterloo 大学刘珏文教授团队 合作，改变思路，聚焦于单线态氧产生的光物理和光化学过程， 通过镧系金属离子添加，助力提升光敏剂单线态氧产率。

杨教授团队对敏化剂的室温发光、低温磷光以及磷光寿命分别进行了表征，同时对 TTA 上转换实验各项参数指标也进行了检测，如 TTA 上转换发光、上转换发光随浓度、功率的变化以及时间分辨发光光谱 等

杨教授团队对敏化剂的室温发光、低温磷光以及磷光寿命分别进行了表征，同时对TTA上转换实验各项参数指标也进行了检测，如TTA上转换发光、上转换发光随浓度、功率的变化以及时间分辨发光光谱等。

Professor Wai’s research is the first to show that synchronous scanning plus the second derivative can distinguish among four likely reduction products in a TNT-based explosive mixture. Synchronous scanning itself has advantages over other identification methods, because it is rapid, sensitive, and selective. Taking the second derivative helps, in addition, to distinguish various products whose spectra overlap. The use of an agent (fluorescamine, in this case) to form a derivative compound reaches a lower limit of detection. HORIBA Jobin Yvon spectrofluorometers are capable of recording and analyzing weak fluorescence such as that presented here.

Professor Wai’s research is the first to show that synchronous scanning plus the second derivative can distinguish among four likely reduction products in a TNT-based explosive mixture. Synchronous scanning itself has advantages over other identification methods, because it is rapid, sensitive, and selective. Taking the second derivative helps, in addition, to distinguish various products whose spectra overlap. The use of an agent (fluorescamine, in this case) to form a derivative compound reaches a lower limit of detection. HORIBA Jobin Yvon spectrofluorometers are capable of recording and analyzing weak fluorescence such as that presented here.

Water-Raman transitions are detectable across a large spectral range. Therefore, the Fluorolog ® demonstrates high sensitivity to fluorescence throughout the UV- visible region; see if your system can handle this task. Kinematically mounted gratings allow optimization of the spectrofluorometer custom applications at various wavelengths. Gratings, blazed at different wavelengths and optimized for different spectral regions, are changed easily with instant alignment. The Fluorolog reaches the statistical noise limit: this means that we provide the best system possible. Water-Raman experiments prove that the bench-top FluoroMax ® is superior in every respect. ?

Ecological and geological studies by means of parallel factor analysis of EEMs has been shown to be a useful application involving HORIBA Jobin Yvon spectrofluorometers.

2022 年 1 月 10 日，南开大学丁丹教授，温州大学黄小波教授、雷云祥博士在 Nature Communications 上发表论文：《Guest-host doped strategy for constructing ultralong-lifetime near-infrared organic phosphorescence materials for bioimaging》。该研究基于主客体掺杂策略，构建出同时具有长波长（600/657– 681/732nm）和长寿命（102–324ms）的有机室温磷光材料，并对该类材料的在组织成像领域进行了深入的研究。

恶唑烷-4-酮通常存在于氧代酰胺的 Norrish II 型转化混合物中，其占比不高，因而产量偏低，导致发展前景受阻。近年来由 Maggers、 Yoon 和 Bach 报道的手性路易斯酸催化合成研究进展引起了专家的注意。受此启发，四川大学冯小明院士课题组首次尝试使用手性路易斯酸催化氧代酰胺 Norrish II 型转化反应，并合成 α-恶唑烷酮化合物（手性恶唑烷-4-酮化合物），且产量较高！这为研究人员进一步推动恶唑烷-4-酮化合物在抗菌活性方面的研发应用，奠定了坚实的第一步。相关文章已发表在 CCS Chemistry。

This technical note describes kinetic fluorescence as an analytical technique to quantify non-fluorescent species. The technique is applied to the determination of thiamine (vitamin B1) in solution. Fluorescence intensity is monitored as thiamine converts to fluorescent thiochrome, and a relationship is established between the rate of increase of intensity and the concentration of thiamine. The technique is shown to have good sensitivity and selectivity.

Besides providing insight into mechanisms of photosynthesis and a possible monitor of reef stability through bleaching, these spectra act as a catalog of coral identity to help distinguish between specific coral types. ?

Besides providing insight into mechanisms of photosynthesis and a possible monitor of reef stability through bleaching, these spectra act as a catalog of coral identity to help distinguish between specific coral types. ?

FLUOROMAX®, with DATAMAX® software, allows the user to monitor wavelengthpairs to characterize processes in living cells and other biological systems. The measurement is feasible in real time because of the FLUOROMAX® spectrofluorometer’s ability to scan rapidly(up to 200 nm s-1)

Polarization measurements using HORIBA Jobin Yvon spectrofluorometers are a sensitive tool for probing many biochemical interactions.

Polarization measurements using HORIBA Jobin Yvon spectrofluorometers are a sensitive tool for probing many biochemical interactions.

Biological processes can be traced through study of gene-expression via a “molecular beacon” (singlestranded DNA, ssDNA), a hairpinshaped oligonucleotide with a fluorophore (donor) and a quencher (acceptor). The hairpin’s stem has two ends of complementary DNA (cDNA) that pair up. When hybridized, the fluorophore and quencher are close, producing little or no fluorescence. Molecular beacons are used to study enzyme interactions, cDNA sequencing, and biosensing.1

令人高兴的是，华东理工大学的田禾院士、马骧教授团 队 近年来一直致力于无定形态纯有机室温磷光材料的研 究，对这一领域有着深刻的理解和认识，并且取得了一 系列突破性进展。近期该团队受邀撰写了关于室温磷光 材料的综述，并发表于 Angew . Chem. Int. 该综述 主要 从无定形态纯有机室温磷光材料的设计思路入手， 总结评述了近年来该领域的一些代表性研究成果和热门 应用 。

A HORIBA Jobin Yvon spectrofluorometer with double grating monochromators for both excitation and emission can be used for noninvasive in-vivo determination of sunscreen PFA via diffuse-reflectance spectroscopy. Because of the extremely low level of stray light that these monochromators allow, our spectrofluorometers can measure highly scattering samples, such as human skin, with excellent sensitivity and precision. Diffuse-reflectance DR) spectra of human skin both with sunscreen and without can be directly measured in vivo and differentiated within minutes. The ratio of spectra with and without sunscreen results in trans- mittance spectra of sunscreens, which allows calculation of PFAs. This non-invasive method provides an alternative diagnostic tool for quick and accurate determination of PFA of sunscreen products.

The Spex ® FluoroMax ®-P spectrofluorometer with integral flash-lamp can perform time-gated spectroscopic separation of mixtures of chemical species.

Use of a HORIBA Jobin Yvon phosphorimeter, including gated delay of signal-acquisition, can reveal extra information, such as FRET, about the physical and chemical properties of materials.

If the region from 850 nm to 1010 nm is important to your work, then the R2658 is an indispensable detector. A water Raman spectrum with the R2658 yields a peak intensity of almost 150 000 counts/s, which is still better than almost any other spectrofluoro- meter—except a standard FluoroMax® or Fluorolog®.

Particle size and composition can control quantum confinement. QDs may assist deep-tissue molecular imaging in living systems, because of their near-IR and far-red fluorescence away from aqueous absorption. QDs also provide absorption coefficients much larger than typical organic dyes. The ultrasensitive FluoroMax spectrofluorometer is useful for research related to nanostructures and materials science.

根据国家卫生研究所2018年公布的一项研究，对于无法手术的癌症类型，光动力学疗法有着非常广阔的应用前景，如从骨骼开始病变的骨肉瘤就是典型。

The fast scanning, ease of operation, and remote sampling capability of the FLUOROMAX® spectrofluorometer make it a natural choice for quantifying UV damage to hair. The data derived there from can be used for the development of treatments to revitalize damaged hair.

基于OLEDs制备的显示屏柔韧性好可弯曲，且相较于LED屏幕更为轻薄、清晰、亮度高色彩鲜艳，且发光效率也更高。市面上常见的曲面电视就是OLED显示屏的绝佳应用案例。可以想见，将这种OLEDs制备的显示屏应用于手机屏制作，降低手机生产成本，或许会为折叠屏手机市场的拓展带来新契机。利用碳纳米点制备OLEDs虽然有种种优势，但其耐久性及对环境的敏感性一直是一个障碍。为克服这些技术难点，肯塔基大学化学系副教授金斗允博士选择先从基础研究做起，使用HORIBA FluoroMax荧光光谱仪的扩展版来研究碳纳米点。

With the use of this fiber-optic adaptor and the pinhole adaptor, many microscopic fluorescence measurements can be achieved. Most microscopes, including Nikon, Olympus, Zeiss, and Leica, are compatible with the Microscope Adapter.