表面等离子共振仪(SPR)(Surface Plasmon Resonance)
表面等离子共振仪(SPR)(Surface Plasmon Resonance)

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Multiskop-SPR

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  • 第17年
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仪器简介:
SPR主要的应用就是在生物领域,
典型的应用比如可以实时观测大分子之间的交互作用,
无须标记,测量时间迅速,利用SPR的主要目的就是想观测什么分子与 
我们感兴趣的分子相结合,结合的时间有多快,结合的有多强。
在未来的几年中相信SPR会在生化,食品,环境领域中都将有长足的发展。

主要特点:
SPR 的三个模式:
1 Theta-Theta角扫描范围为20-120度,这个范围对激励大多数基于 Kretschmann 结构的表面等离子来说是足够的。配合相应的软件,角度计可以精确到2/1000度。
2 动力学模式:您可以在毫秒量级的时间跟踪测量吸附的动力学过程,附件提供了一种应用于动力学模块的新方法,跟踪时间为50毫秒而角度精确到1/1000度,方法消除了激光信号的漂移,这点是非常重要的。
3 成像模式: 您可以通过成像系统直接成像。

厂家可以提供各种SPR 传感片

相关方案

  • Surface plasmon resonance (SPR) spectroscopy is a widely used optical reflection technique for the characterization of thin films. The central quantity of SPR spectroscopy is the surface plasmon coupling angle as a characteristic signature of the prevailing interfacial architecture. Adsorption processes lead to a shift of the surface plasmon resonance which is in the thin film limit directly proportional to the corresponding mass coverage. The aim of any SPR instrument is a precise measurement of the coupling angle with a sufficient high time resolution that fast kinetic processes can be monitored. In this paper, we compare two promising methods, an established one,the reflectivity tracking and a fairly new one, the mismatch tracking. Reflectivity tracking simply records the intensity of the light in the vicinity of the coupling angle. The shift of the plasmon modifies the intensity at the detector which can subsequently be used for data analysis. Mismatch tracking is more complex. Light is focused with a lens onto the prism base and the reflected light is detected via a bicell detector. The upper and lower cell integrate over a well-defined angular range of the fan of rays produced within the focus. The mismatch in the intensity between the upper and lower segments is evaluated and used to retrieve the unknown film parameter. In this contribution we suggest some decisive variations of the originally proposed scheme and demonstrate that the modified mismatch scheme yields a significantly higher sensitivity than the original one. Furthermore, it is demonstrated that this scheme is a superior alternative to reflectivity tracking.

    其他 2008-04-25

  • Surface plasmon resonance (SPR) spectroscopy is a widely used optical reflection technique for the characterization of thin films. The central quantity of SPR spectroscopy is the surface plasmon coupling angle as a characteristic signature of the prevailing interfacial architecture. Adsorption processes lead to a shift of the surface plasmon resonance which is in the thin film limit directly proportional to the corresponding mass coverage. The aim of any SPR instrument is a precise measurement of the coupling angle with a sufficient high time resolution that fast kinetic processes can be monitored. In this paper, we compare two promising methods, an established one,the reflectivity tracking and a fairly new one, the mismatch tracking. Reflectivity tracking simply records the intensity of the light in the vicinity of the coupling angle. The shift of the plasmon modifies the intensity at the detector which can subsequently be used for data analysis. Mismatch tracking is more complex. Light is focused with a lens onto the prism base and the reflected light is detected via a bicell detector. The upper and lower cell integrate over a well-defined angular range of the fan of rays produced within the focus. The mismatch in the intensity between the upper and lower segments is evaluated and used to retrieve the unknown film parameter. In this contribution we suggest some decisive variations of the originally proposed scheme and demonstrate that the modified mismatch scheme yields a significantly higher sensitivity than the original one. Furthermore, it is demonstrated that this scheme is a superior alternative to reflectivity tracking.

    其他 2008-04-25

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