赛默飞Q Exactive HF LCMS 系统

台式四极杆超高静电场轨道阱质谱技术在鸟枪法蛋白质组学中的改进

The Q Exactive HF benchtop Orbitrap mass spectrometer shows excellent performance for the analysis of complex proteolytic protein digests, analyzed in 30, 60 and 120 min run times. Average data dependent scan speed of 16-18 Hz over the whole gradient is obtained on the Q Exactive HF MS using a HeLa proteolytic digest. Q Exactive HF MS significantly improves productivity for shotgun, bottom-up analysis compared to the Q Exactive Plus MS: ? 2x shorter analysis time with approx. the same no of unique peptide identifications. ? Up to 50% more identified peptides with shorter gradients.

Higher Resolution LCMS and MSMS Analysis of Lipid Extracts Using Benchtop Orbitrap-based Mass Spectrometers and LipidSearch Software

Four different total lipid extracts were profiled and are suitable for benchmarking lipidomics experiments, separation conditions and instrumental optimization. Higher resolution and speed of Q Exactive HF provides more confident identification and relative quantification in a single positive and negative ion run. At least 20% more lipid ID’s were obtained using a ultra-high field benchtop Orbitrap. LipidSearch provides automated identification of more than 500 lipid species in a single LC-ddMS2 experiment, and alignment of multiple samples gives over 1000 lipid species.