仪器种类: 四极杆-飞行时间
产地类别: 进口
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the timsTOF™ Pro Mass Spectrometer Enables the Revolutionary PASEF Method for Next-Generation Proteomics
Unique Trapped Ion Mobility Spectrometry (TIMS) Adds New Dimension for Robust, Faster Proteome Analysis with Higher Sensitivity
Mass spectrometry (MS)-based proteomics has become a powerful technology for the identification and quantification of thousands of proteins. However, the coverage of complete proteomes is still very challenging due to the limited speed, sensitivity and resolution of current mass spectrometers.
The timsTOF Pro uses the Parallel Accumulation Serial Fragmentation (PASEF) acquisition method to provide extremely high speed and sensitivity to reach new depths in shotgun proteomics and phosphoproteomics using low sample amounts.
A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are simultaneously released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation or PASEF.
The timsTOF Pro powered by PASEF offers sequencing speed > 100 Hz without losing sensitivity by synchronizing the quadrupole isolation mass window with the elution time of the specific peptide packages from the TIMS funnel.
An open-file data format allows researchers to directly work with the raw data and use industry leading software. MaxQuant has been adapted to manage 4D features in the space spanned by retention time, ion mobility, mass, and signal intensity which benefit the identification and quantification of peptides, proteins, and posttranslational modifications. PEAKS Studio combines de novo sequencing with tradtional databbase searches and is optimized for processing timsTOF raw data.
Technical Details
Outstanding hardware performance
The second generation dual TIMS analyzer is optimized for shotgun proteomics needs. Due to its unique geometry ions are released dependent on their mobility from the second section of the TIMS analyzer while the further incoming ions can be accumulated in parallel in the first part of the TIMS analyzer. With the parallel accumulation technology a duty cycle up to 100% can be achieved resulting in no ion loss.
In the timsTOF Pro an advanced segmented quadrupole mass filter is used for high ion transmission and isolation efficiency. The quadrupole mass position is synchronized with the elution times of the specific ions from the TIMS analyzer. Due to its ultra-high mass position switching time (< 1 ms) it enables the best performance for the PASEF method.
TIMS
Trapped Ion Mobility Spectrometry (TIMS) is first and foremost a separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization.
PASEF
With speed in mind, Bruker experts redesigned MS/MS technology to meet the requirements of shotgun proteomics. Peptide ions are separated using trapped ion mobility spectrometry, eluted (~ 100 ms) and detected in the QTOF, generating the TIMS MS heat map (A). In the PASEF method (B) the same TIMS separation is used with the quadrupole isolating a certain ion species during its elution and immediately shifting to the next precursor. Parent and fragment spectra are aligned by mobility values. With the Parallel Accumulation Serial Fragmentation (PASEF) Technology >100 Hz sequencing speed can be achieved. With the PASEF method the MS/MS spectra quality of the low abundant peptides can be increased by selecting them several times.
布鲁克solariX磁共振质谱仪
型号:solariX 面议布鲁克 compact 高分辨飞行时间质谱
型号:compact 面议布鲁克scimaX™磁共振质谱仪
型号:scimaX™ 面议布鲁克 maXis II 飞行时间串联质谱
型号:maXis 面议专访:Sally-Ann Poulsen’s laboratory at the Griffith Institute for Drug Discovery (GRIDD) uses advanced MRMS technology to support their research in small molecule drug discovery. 位于澳大利亚昆士兰州的Griffith大学药物发现研究院GRIDD,拥有庞大的天然产物资源库,一个由澳大利亚各种植物、真菌和无脊椎动物的天然提取物构成的药物发现平台,其中包括10000个提取物库和50000个天然产物组分库,GRIDD的科学家将布鲁克solariX XR磁共振质谱仪用于基于片段药物筛选技术探寻隐藏的新药,实现了对药物研发快速分析、高效率和降低成本的要求,solariX XR的超高分辨率性能给蛋白质小分子复合物分析提供可靠高效的结果。
In this application note, we describe a workflow for MALDI Mass Spectrometry Imaging (MSI) of proteins from a variety of FFPE tissues. The method comprises steps of paraffin removal and antigen retrieval followed by on-tissue digestion and deposition of MALDI matrix.
• Mass-MetaSite™ software and the described workflow proved to be a suitable, less laborious and time consuming procedure compared to manual data evaluation. • The described approach can be helpful for updating screening methods with metabolite information. • Having metabolites included in screening methods is necessary when dealing with analytes that are extensively metabolized such as synthetic cannabinoids. • Identification of metabolites along with parent compounds can serve as a plausibility check and may help in estimating the time of the last drug uptake.
Sometimes it's not enough to know how much drug has reached a particular tissue. Sometimes you need to know where in that tissue the drug has gone. To answer that and other questions, pharmaceutical companies are turning to imaging mass spectrometry. It allows pharma scientists to collect information about drug distribution much earlier in the discovery process than previously possible. The resulting pictures are helping drug developers prevent toxicity and off-target effects.
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