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Ekspla CARS 相干反斯托克斯拉曼显微光谱仪
Ekspla CARS 相干反斯托克斯拉曼显微光谱仪
Ekspla CARS 相干反斯托克斯拉曼显微光谱仪
Ekspla CARS 相干反斯托克斯拉曼显微光谱仪
Ekspla CARS 相干反斯托克斯拉曼显微光谱仪
Ekspla CARS 相干反斯托克斯拉曼显微光谱仪
Ekspla CARS 相干反斯托克斯拉曼显微光谱仪
Ekspla CARS 相干反斯托克斯拉曼显微光谱仪
Ekspla CARS 相干反斯托克斯拉曼显微光谱仪
Ekspla CARS 相干反斯托克斯拉曼显微光谱仪

Ekspla CARS 相干反斯托克斯拉曼显微光谱仪

¥100万 - 150万

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CARS­

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欧洲

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北京欧兰科技发展有限公司
  • 金牌
  • 第17年
  • 一般经销商
    • 营业执照已审核
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核心参数

仪器种类： 显微共焦拉曼光谱

产地类别： 进口

光谱范围： 740-4000cm-1

光谱分辨率： 1cm-1

空间分辨率： 0.5微米

最低波数： 740cm-1

光谱重复性： 1%

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为您推荐相似的激光拉曼光谱(RAMAN)

产品介绍 相关方案 用户评论 问商家

特色

● 免标记（Label-free）光学成像

● 用于活体细胞研究获得无光学漂白信号

● 700 – 4000 cm⁻¹宽带可调谐

● 高灵敏度

● 荧光干扰信号最低

● 亚波长尺度空间分辨率

● 系统集成 Ekspla 最新型PT259 激光器

● 系统可方便地转换成双光子激发荧光和倍频发生显微光谱仪

应用

● E-CARS, F-CARS, P-CARS 等各种光谱学研究

● 组分对象选择性/特异性显微测量学

● 样品3D成像

● 动态活细泡成像

● 活细胞处理/加工的长周期监控

● 医学生物研究中的无损测量

● 用户的个性化应用领域…

    相干反斯托克斯拉曼光谱也称作相干反斯托克斯拉曼散射光谱(CARS)。它是一种主要用于化学和物理及相关领域的光谱技术。和拉曼光谱类似，其对于分子相同的振动能级特征，典型的是化学键的原子间振动，具有较高的检测灵敏度。和普通拉曼光谱学不同之处在于，CARS 采用多个光子协同工作共同来检测分子的振动特性，因此其产生的信号光子之间彼此具有相关性。因此CARS信号强度通常比自发拉曼散射信号要高若干个量级。
    CARS 是一种涉及三路激光束的三阶非线性光学过程: 一路频率为 ωpump的泵浦光,一路频率为ωStokes的斯托克斯光和一路频率为 ωprobe的探针光. 这些路激光和样品相互作用并产生一路相干光学信号，其频率处于反斯托克斯位置：
    ωCARS = ωpump - ωStokes + ωprobe.

    当泵浦光 ωpump 和斯托克斯光ωStokes频率之差接近分子某个振动跃迁频率 ωvib 时，CARS 信号会发生共振增强。

相关方案

综合石油/化工其他环保生物产业材料
  • 等离子体表面改性聚二甲基硅氧烷中动力学过程研究,和频光谱,SFG检测方案(其它光谱仪)
    In numerous applications in microfluidics, cell growth, soft lithography, and molecular imprinting, the surface of poly(dimethylsiloxane) (PDMS) is modified from a hydrophobic methyl-terminated surface to a hydrophilic hydroxylterminated surface. In this study, we investigated molecular structural and orientational changes at the PDMS-air interface in response to three commonly used surface modification processes: exposure to long-wavelength ultraviolet light (UV), exposure to short-wavelength UV that generates ozone (UVO), and exposure to oxygen plasma (OP). The surfaces of two PDMS compositions (10:1 and 4:1 of base polymer/curing agent) were probed during modification, using monolayer-sensitive IR + visible sum frequency generation (SFG) vibrational spectroscopy, with two different polarization combinations. During PDMS surface modification, the peak intensities of CH3 side groups and CH2 cross-link groups decreased, while peak intensities of Si-OH groups increased. There was no significant change in the average orientation of the CH3 groups on the PDMS surface during modification. The concentration of CH3 groups on the surface decreased exponentially with time, for all three UV, UVO, and OP modification processes, with first order kinetics and time constants of approximately 160, 66, and 0.3 min, respectively. At steady state, residual CH3 groups were detected at the PDMS surface for UV and UVO treatments; however, there were negligible CH3 groups detected after OP modification.

    石油/化工 2008-05-10

  • 金/溶液界面,十八烷基硫醇,单分子层膜构象中SFG光谱,和频光谱检测方案(其它光谱仪)
    Sum frequency generation (SFG) spectroscopy using internal reflection geometry was employed to investigate the conformational order of octadecanethiol (ODT) monolayer on gold thin films in an electrolyte solution. This approach is convinced to be useful to study the molecular structure on the electrode/solution interface under electrochemical condition.

    石油/化工 2008-05-07

  • 十八烷基三氯硅烷,OTS,单层膜改性熔融石英中表面水分子结构,pH值相关性,和频光谱（SFG）研究检测方案(其它光谱仪)
    The interface-sensitive spectroscopic method, sum frequency generation (SFG), has been used to investigate the interfacial water structure on a fused quartz surface modiDed by an octadecyltrichlorosilane (OTS) self-assembled monolayer in phosphate bu†ered solutions at various pHs. The experimental results demonstrate that the water molecules at the quartz/OTS surface Ñip while the water molecules at the OTS surface maintain their orientation when the solution pH is changed from neutral to acidic. The results show that most of the silanol groups still exist on the fused quartz surface even after a silane coupling reaction of OTS under the reported experimental conditions.

    石油/化工 2008-04-26

  • 聚苯胺中聚苯胺结构检测方案(其它光谱仪)
    Infrared-visible sum-frequency generation (SFG) vibrational spectra were obtained from the conducting form of polyaniline (PANI), emeraldine salt (ES). Observation of the SFG signal indicated the presence of oriented non-planar structures in ES. It was suggested that the additional resonant enhancement due to the absorption of the visible beam by the polymer film operated in the investigated system. Resonances characteristic to different structural units, benzenoid rings and polarons, were detected in the SFG spectra. The C-C stretching and C-H deformation vibrations of benzenoid rings were detected at 1623 and 1192 cm–1, while the polaronic ν(C~N+) vibrational mode was observed at 1336 cm–1. The stretching vibration of N-H bond was detected at 3273 cm–1 in the ssp-polarized SFG spectrum, indicating that the neighboring polymer chains in ES are connected by the N-H•••N interchain bridges

    石油/化工 2008-04-26

  • 二甲基硅油Poly(dimethylsiloxane)中紫外和表面等离子体改性动力学检测方案(其它光谱仪)
    In numerous applications in microfluidics, cell growth, soft lithography, and molecular imprinting, the surface of poly(dimethylsiloxane) (PDMS) is modified from a hydrophobic methyl-terminated surface to a hydrophilic hydroxylterminated surface. In this study, we investigated molecular structural and orientational changes at the PDMS-air interface in response to three commonly used surface modification processes: exposure to long-wavelength ultraviolet light (UV), exposure to short-wavelength UV that generates ozone (UVO), and exposure to oxygen plasma (OP). The surfaces of two PDMS compositions (10:1 and 4:1 of base polymer/curing agent) were probed during modification, using monolayer-sensitive IR + visible sum frequency generation (SFG) vibrational spectroscopy, with two different polarization combinations. During PDMS surface modification, the peak intensities of CH3 side groups and CH2 cross-link groups decreased, while peak intensities of Si-OH groups increased. There was no significant change in the average orientation of the CH3 groups on the PDMS surface during modification. The concentration of CH3 groups on the surface decreased exponentially with time, for all three UV, UVO, and OP modification processes, with first order kinetics and time constants of approximately 160, 66, and 0.3 min, respectively. At steady state, residual CH3 groups were detected at the PDMS surface for UV and UVO treatments; however, there were negligible CH3 groups detected after OP modification.

    石油/化工 2016-10-31

  • 水中振动和频光谱检测方案(其它光谱仪)
    We present a direct comparison of phase sensitive sum-frequency generation experiments with phase reconstruction obtained by the maximum entropy method. We show that both methods lead to the same complex spectrum. Furthermore, we discuss the strengths and weaknesses of each of these methods, analyzing possible sources of experimental and analytical errors. A simulation program for maximum entropy phase reconstruction is available at: http://lbp.epfl.ch/.

    石油/化工 2016-10-31

  • 空气/水界面中和频光谱检测方案(激光拉曼光谱)
    he nature of water's hydrogen-bonding network is a vital in fluence on the chemistry that occurs at interfaces, but a complete understanding of interfacial water has proven elusive. Even-order nonlinear optical spectroscopies, such as vibrational sum frequency generation (VSFG) spectroscopy and heterodyne detected phase-sensitive sum frequency generation (PS-SFG) spectroscopy, are inherently surface specific. With the advent of advances in these spectroscopic techniques, researchers can now explore many longstanding questions about the dynamics and structures present at the vaporwater and watersolid interfaces. Of special interest to the atmospheric chemistry community is the accommodation of ions and solutes by water's hydrogen-bonding network. A better understanding of how ions and solutes behave in hydrogen-bonded water has afforded a fresh perspective of aqueous aerosols, because the interactions involved therein drive phenomena such as the hydrolysis of atmospheric chemical species. In this Account, we present work from our laboratory focusing on applying VSFG and the recently developed PSSFG techniques to probe the perturbation ofwater's hydrogen-bonding network at the vaporwater interface by a variety of ions and solutes. We also present very recent results from our laboratory on the direct observation of the adsorption of ions at the waterCaF2 interface.

    石油/化工 2016-10-17

  • 金属/有机物界面中和频光谱检测方案(其它光谱仪)
    Recent advances in the performance of organic semiconductors such as organic light emitting diodes (OLEDs), organic field-effect transistors (OFETs), and organic solar cells have been dramatic. In particular for OLEDs, stability and durability have improved to levels that warrant their application in everyday life. In organic devices, the charge carriers, both electrons and holes, often have to be injected through organic/electrode interfaces. Therefore, an understanding of the interaction between metal electrode and organic molecules is quite important, because the electronic properties of metal/organic interface directly affect the performance of the OLEDs.

    石油/化工 2016-10-17

  • 便携式发电机的催化微反应器中拉曼,LIF信号检测方案(流量计)
    The test rig employed in this study consisted of a high-pressure cylindrical steel vessel with a length of 1.8 m and an internal diameter of 0.28 m. Visual inspection and accessibility of the reactor assembly was achieved via a 50 mm diameter quartz window at the rear flange of the vessel and two 350 mm long and 50 mm high quartz windows at the vessel sides. The test setup (Fig. 2.1) consisted of a channelflow catalytic reactor, which was mounted inside the high-pressure cylindrical vessel. The reactor comprised two horizontal Si[SiC] plates with a length (x) of 300 mm, width (z) of 104 mm and thickness of 9 mm; the plates were positioned 7 mm apart (y). The other two sides of the reactor were formed by two 3-mm-thick vertical quartz windows.

    其他 2016-11-16

  • 掺杂Nd3+离子的CaWO4晶体,Hexobenzocoronane(HBC)粉末中拉曼散射检测方案(CCD相机)
    A high level of fluorescence background signal rejection was achieved for solid and powder samples by using a combination of simple low-resolution spectrograph and ultrafast gated charge coupled device ~CCD! camera. The unique timing characteristics of the CCD camera match exceptionally well to characteristics of a Ti:sapphire oscillator allowing fast gated light detection at a repetition rate of up to 110 MHz, making this approach superior in terms of the duty cycle in comparison with other time-resolved Raman techniques. The achieved temporal resolution was about 150 ps under 785 nm Ti: sapphire laser excitation. At an average excitation power up to 300 mW there was no noticeable sample damage observed. Hence, the demonstrated approach extends the capabilities of Raman spectroscopy regarding the investigation of samples with a short fluorescence lifetime. The combination of a spectrometer and a gated CCD camera allows simultaneous study of spectral and temporal characteristics of emitted light. This capability opens an exciting possibility to build a universal instrument for solving multitask problems in applied laser spectroscopy

    其他 2013-02-13

  • 雾沫中雾化形成在静电旋转钟镀膜中的应用检测方案(激光粒度仪)
    Electrostatic, rotating bell (ESRB) application is one of the most important coating application techniques for industries with demanding specifications for optical attractiveness of coatings, such as automotive. The ESRB process involves production of droplets using a high-speed rotating bell, which are subsequently transported to the substrate being coated via shaping air [1-3]. An electrical potential is applied between the bell and the substrate which further helps droplet atomization and transport. This research investigates the effects of inertia, centrifugal force, drag force, and electrostatic force on the atomization mechanism and particle size distribution using an automotive OEM base coat formulation. Coating flow rate (CFR), shaping air flow rate (SAFR), bell speed (BS), and electrostatic potential (EP) were used as primary parameters to create various atomization conditions and particle size distributions. The atomization mechanism, ligament formation, and particle size distribution were measured using high-speed laser shadowography and image processing. The effects of governing forces and particle size generated on efficiency of droplet transfer to the substrate and optical appearance of the coatings were studied to generate operating windows for optimum process efficiency and appearance.

    其他 2012-01-24

  • 电容中相位分辨光学发射光谱学：一种用于研究电容射频放电中电子动力学的非介入式诊断方法检测方案(CCD相机)
    Various types of capacitively coupled radio frequency (CCRF) discharges are frequently used for different applications ranging from chip and solar cell manufacturing to the creation of biocompatible surfaces. In many of these discharges electron heating and electron dynamics are not fully understood. A powerful diagnostic to study electron dynamics in CCRF discharges is phase resolved optical emission spectroscopy (PROES). It is non-intrusive and provides access to the dynamics of highly energetic electrons, which sustain the discharge via ionization, with high spatial and temporal resolution within the RF period. Based on a time dependent model of the excitation dynamics of specifically chosen rare gas levels PROES provides access to plasma parameters such as the electron temperature, electron density and electron energy distribution function (EEDF). In this work the method of PROES is reviewed and some examples of its application are discussed. First, the generation of highly energetic electron beams by the expanding sheath in geometrically symmetric as well as asymmetric discharges and their effect on the EEDF are investigated. Second, the physical nature of the frequency coupling in dual frequency discharges operated at substantially different frequencies is discussed. Third, the generation of electric field reversals during sheath collapse in single and dual frequency discharges is analysed. Then excitation dynamics in an electrically asymmetric novel type of dual frequency discharge is studied. Finally, limitations of PROES are discussed.

    其他 2011-03-03

  • 水,室温离子液体,表面阳离子取向中振动和频发生光谱学研究,SFG光谱,和频光谱检测方案(其它光谱仪)
    Sum frequency generation vibrational spectroscopy was used to determine the orientation of the cations in a room-temperature ionic liquid at the air-liquid interface. The ionic liquid that was studied was 1-butyl-3- methylimidazolium bis-trifluoromethylsulfonimide, [BMIM]+[imide]-. The orientation of the cation, [BMIM]+, was studied as a function of water pressure in the vapor over the range of 10-5 to 200 Torr. At water pressures below 10-4 Torr, the imidazolium ring was oriented parallel to the surface plane. On increasing the water pressure to greater than 10-4 Torr, the imidazolium ring tipped up from the surface with an angle along the surface normal of 40-55°. No water signal was observed in the spectra obtained at the water pressures studied here.

    环保 2008-05-06

  • 矿物/水界面中表面和频光谱（SFG)检测方案(其它光谱仪)
    Interactions of water and aqueous solutions with mineral surfaces play an important role in a variety of environmental processes. These processes include soil formation, cycling of chemical elements in nature, mobility of heavy metals and other contaminants as well as nutrients, and surface growth of microorganisms (Brown et al., 1999). Such interactions are affected by solution pH, the presence of dissolved ions, and the surface structure of the solid. The objective of this project is to use available experimental techniques and theoretical approaches to develop a comprehensive microscopic picture of how water and solutes interact with mineral surfaces.

    环保 2008-05-01

  • 细胞,生物医学样品中 成像检测方案(激光拉曼光谱)
    In this work multimodal operation of commercially available CARS microspectrometer (EKSPLA CARSCOPE) equipped with a picosecond dual wavelength laser source (EKSPLA PT259) was demonstrated for the imaging of various biological objects using coherent anti‑Stokes Raman scattering (CARS), two photon excited fluorescence (TPEF) and second harmonic generation (SHG) contrast mechanisms. Obtained CARS‑images of yeast cell and flower pollen clearly demonstrate label‑free and 3D imaging capabilities of CARS technique. Multi‑contrast images of green algae and plants containing starch demonstrate a variety of research facilities, where the method can be applied.

    生物产业 2012-06-26

  • NADPH中选择性探测多核形细胞中NADPH氧化酶检测方案(CCD相机)
    NADPH oxidase (NOX2) is a multisubunit membrane-bound enzyme complex that, upon assembly in activated cells, catalyses the reduction of free oxygen to its superoxide anion, which further leads to reactive oxygen species (ROS) that are toxic to invading pathogens, for example, the fungus Aspergillus fumigatus. Polymorphonuclear cells (PMNs) employ both nonoxidative and oxidative mechanisms to clear this fungus from the lung. The oxidative mechanisms mainly depend on the proper assembly and function of NOX2. We identified for the first time the NAD(P)H-dependent enzymes involved in such oxidative mechanisms by means of biexponentialNAD(P)H-fluorescence lifetime imaging (FLIM). A specific fluorescence lifetime of 3670±140 picoseconds as compared to 1870 picoseconds for NAD(P)H bound to mitochondrial enzymes could be associated with NADPH bound to oxidative enzymes in activated PMNs. Due to its predominance in PMNs and due to the use of selective activators and inhibitors, we strongly believe that this specific lifetime mainly originates from NOX2. Our experiments also revealed the high site specificity of the NOX2 assembly and, thus, of the ROS production as well as the dynamic nature of these phenomena. On the example of NADPH oxidase, we demonstrate the potential of NAD(P)H-based FLIM in selectively investigating enzymes during their cellular function.

    生物产业 2011-03-27

  • 低碳钢表面,硫醇,自组装,单层膜中显微成像,自组装,单层膜的空间分辨,表面分析检测方案(其它光谱仪)
    Sum frequency generation imaging microscopy has been used to investigate a self-assembled monolayer of an alkanethiol (octadecanethiol, ODT) on a mild steel surface. The images are used to analyze the orientation of the alkanethiol monolayer and the distribution of orientational angles as well as defects in the film. The results show that, on average, ODT forms an ordered monolayer on mild steel when compared to the same monolayer on gold. However, the image analysis suggests that the distribution of tilt angles and conformational defects is greater for ODT on a mild steel surface compared to ODT/Au.

    材料 2008-04-26

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Ekspla激光拉曼光谱CARS­的工作原理介绍

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Ekspla CARS 相干反斯托克斯拉曼显微光谱仪信息由北京欧兰科技发展有限公司为您提供，如您想了解更多关于Ekspla CARS 相干反斯托克斯拉曼显微光谱仪报价、型号、参数等信息，欢迎来电或留言咨询。
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