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Bruker PreON 自动化样本前处理
基本信息:
价格:面议
供货周期:一个月以上
品牌:布鲁克
货号:

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产品介绍

The challenge:

How do you bring further standardization, reproducibility and hands on time savings to proteomics sample preparation for  LC-MS analysis when you have already successfully addressed  the many challenges of time, sample contamination, sample loss and reproducibility?

The solution:

Automate! Introducing the PreON® platform; built to address the demands of reproducible, robust and sensitive proteomics sample preparation for LC-MS analysis. It allows research labs to automate proteomics processing at the push of a button decreasing the hands-on time from 1 hour (iSTiST-NHS and iST-BCT sample preparation) to about 5 minutes!

Apply! Simple, streamlined workflows

1. Samples
Samples should be liquid, homogenates or a soft pellet.
2. Lyse, reduce, alkylate
Add LYSE reagent, incubate for 10 mins at high temperature.
3. Digest and label
Cool, add DIGEST reagent, incubate for 60 mins at 37°C. Label according to vendor instructions.
4. Purify
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5. LC-MS analysis
Inject and analyze to see improved peptide identities.

Results

The PreON® eliminates manual processing errors and pipetting variability, improving quality and reproducibility. Spend less time preparing your samples, increase throughput and achieve better results.

The PreON® significantly reduces variability in chemically-labeled proteomics sample preparation by reducing manual steps and eliminating processing errors. Spend less time preparing your samples while achieving better results.

PreOmics®'

PreON®

values

Smile

Improved sample quality
  • High reproducibility: R2>0.9

  • Working range: 1-100 µg

  • Increased peptide and protein identifications

  • Benchtop footprint (0.81 x 0.65 x 0.62 m HxWxD, weight 71.5kg)

  • Installation and training included

Relax

Sample preparation fully automated
  • Can prepare up to 12 unlabeled and 16 labeled samples (standard configuration)

  • 18 labeled samples can now be processed with the new TMTproTM 18-plex upgrade set*

  • True walk-away automation for label-free and chemical labeling workflows

  • Eliminates manual processing steps (only 5 mins hands on time)

  • Works seamlessly with pre-loaded PreOmics® protocols

  • PreON® is installed and running samples in less than a day

  • Intuitive touch screen menus

  • Overnight evaporation option

  • Range of service plans available

Realize

Cleaner peptides ready for analysis
  • Dual clean-up step removes hydrophilic and hydrophobic contaminants

  • Achieve ready-to-measure peptides

  • Reduce mass spectrometry downtime

  • Optical and ultrasonic detectors for accurate labware and liquid detection

  • Onboard centrifuge and heater shaker

  • Optional pauses to help maintain TMT efficiency and conserve TMT reagents

  • CE and CSA compliant



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