样品制备在基于质谱的蛋白质组学工作流程中至关重要，因为它是方法流程可重复的关键因素。然而起始材料的多样性对样本制备带来了一定挑战，如不同的细胞和组织类型。为解决这一问题，PreOmics 基于其创新的 in-StageTip（ iST ）技术，提供了一体化样品制备解决方案。该解决方案易操作、通用性强，而且能够兼容各种样本类型。iST 技术将复杂的工作流程简化为只需三步操作流程的试剂盒，节省时间，提高处理效率。无论是手动、半自动还是全自动，标准化且可重复的iST试剂盒都能在 2 小时内高效地完成蛋白质组学样品制备，便于质谱分析。
iST 技术无论采用何种样品，都具有出色的性能。
（A）Ramos B 细胞，用 iST 制备样品，然后通过 LC-MS 分析鉴定出可重复的大量蛋白质组。（B）使用 BeatBox 或对照装置将不同类型的小鼠组织匀浆，然后使用 iST 技术制备样品，通过 LC-MS 进行分析。无论什么组织类型，使用 BeatBox 匀浆都可以鉴定出大量蛋白质，并提高蛋白质鉴定量。
Common mass spectrometry (MS)-based proteomic workflows are multistep processes employing different methodologies and instrumentation. The issues associated with this approach are the hands-on time required, and a lack of standardization.
Sample preparation is one of the most critical steps in MS-based proteomic workflows, but the variability of the starting materials (cell/tissue type and protein levels) can be challenging. There is a need for easy-to-use and automatable workflows, along with all-in-one sample preparation solutions, to improve the results' consistency and reliability.
PreOmics® has developed innovative, flexible, and easy-to-use all-in-one solutions for a number of different sample types, based on its in-StageTip (iST) technology. Pre-cleaning steps, supplied as add-ons, can remove contamination such as detergents. Fractionation steps downstream in the sample preparation process allow in-depth analysis of purified peptides. The iST technology provides researchers with innovations in devices and processes, allowing them to achieve reliable, robust and reproducible sample preparation with increased peptide and protein ID in record time.
Sample preparation for MS-based proteomics in a single enclosed device uses a three-step workflow, eliminating or combining stages, in a 2-hour time window.
Starting material includes prokaryotic and eukaryotic cells, mammalian and plant tissues, and biological fluids (e.g. serum, plasma).
Our iST kits are simple to use and include all required buffers, reagents and protocols. All researchers need is standard lab equipment.
Want to further increase reproducibility and standardization, while reducing hands-on time (down to five minutes) and human error? Relax and let our PreON automation platform do the work for you.
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