海狸核酸提取磁珠质粒提取磁珠试剂盒
质粒提取磁珠试剂盒BeaverBeads™ Mini Plasmid Kit采用超顺磁性磁珠和优化的缓冲液体系,可方便、快捷地从大肠杆菌中提取超螺旋质粒。提取过程采用磁性分离,菌体裂解后不需要通过高速离心去除杂质,有利于实现高通量操作。提取的质粒可用于酶切、测序、PCR等后续操作。 产品名称编号规格包装BeaverBeads™ Mini Plasmid Kit70402-100100 rxnsBeaverBeads™ Mini Plasmid Kit70402-500500 rxns操作简单:菌体裂解后无需离心或抽滤;轻松实现高通量:适用于在96孔板中操作;产物纯度高:A 260/280=1.7~2.0。应用实例Agarose gel analysis of plasmids. Plasmid 1~Plasmid 4 were propagated in DH5α. Plasmid 5~Plasmid 7 were propagated in BL21(DE3) pLysS, TOP10 and JM109, respectively.M: Thermo Scientific GeneRuler 1kb DNA Ladder (# SM0311)A: Plasmid extracted by BeaverBeadsTM Mini Plasmid Kit (without centrifugation)B: Plasmid extracted by BeaverBeadsTM after centrifugationC: Plasmid extracted by spin column after centrifugation purifie如上图所示,采用本产品提取的质粒DNA,纯度及超螺旋的构象都能达到传统竞争产品的水平,由于提取过程中采用磁珠法去除裂解液中的杂质(A),质粒DNA得率会略低于离心后再提取的样品(B、C),但整个提取过程避免了高速离心步骤,有利于实现高通量及自动化操作。