数字毫秒计

The ideal fluorescence microscope illuminator for quantitative intracellular ion research of Fura-2 [Ca++], BCECF [pH], SBFI [Na+], FRET and much moreThe patented DeltaRAM™ X microscope Illuminator is the ideal fluorescence illuminator for quantitative intracellular ion research. It utilizes a galvanometer based random access monochromator that can switch between any wavelength in 2 milliseconds. The DeltaRAM™ X is a complete, self contained, illuminator that includes a power supply, high intensity xenon light source, DeltaRAM™ X monochromator, TTL shutter and flexible liquid light guide. The wavelength position is a simple analog voltage control. All you need to add is a microscope adapter for your fluorescence microscope and a USB or PCI DAC interface depending on the software you will be using to drive the illuminator. The DeltaRAM was developed and patented by our sister company Photon Technology International (PTI) with hundreds of installed systems around the world. PTI is the pioneer in the quantitative ratio fluorescence marketplace having introduced the first patented ratio illuminator, the Deltascan™ , shortly after the first Fura-2 publication. The DeltaRAM™ X is the newest standard illuminator used with the complete line of PTI EasyRatioPro quantitative fluorescence imaging systems, as well as the PTI RatioMaster™ millisecond PMT photometry system. The DeltaRAM™ X is widely recognized as the multi-wavelength illuminator of choice with outstanding reliability and customer support. For a list of publications citing the DeltaRAM refer to our Applications Page.Compatible DeltaRAM™ X vendor and software platformsThe DeltaRAM™ X can be controlled directly with a variety of third party imaging and instrument software, and OBB provides a number of USB and PCI driver interfaces to support these packages. Photon Technology International: RatioMaster, EasyRatioProMolecular Devices: MetaMorph, MetaFluor Nikon Instruments: ElementsMicro-ManagerOpen Source Microscopy SoftwareNational Instruments: LabVIEWMeasurement Computing DAC’sBetter than a Filter Wheel:Key benefits of the DeltaRAM™ X versus traditional filter wheel illuminatorsMuch faster wavelength switching timesVibration isolation Precise selection of any excitation wavelength and any spectral bandwidthMaximum dynamic range for ratiometric dyes (Fura-2 Rmax/Rmin = 40 with DeltaRAM™ X)Excitation wavelength scanningMuch greater value for about the same priceFeatured Technical NoteAdvantages of Using a Scanning Monochromator for Ratio Fluorometric Experiments(233 KB PDF) Key Components of the DeltaRAM™ XPowerArc™ xenon arc lamp illuminator and power supplyDeltaRAM Random Access MonochromatorLiquid light guideMicroscope adapter (optional)USB or PCI interface (optional) PowerArc™ Lamp Housing The DeltaRAM™ X is powered by a proprietary PowerArc™ lamp housing that requires no cooling or venting. It uses an on-axis ellipsoidal reflector for light collection to collect 70% of the radiant energy from the 75 watt xenon arc lamp compared to only 12%collection efficiency for traditional microscope fluorescence illuminators. The ellipse literally wraps around the arc lamp, collecting 5 to 6 times more output power than from a conventional lamp housing. This outstanding collection efficiency and brightness assures the delivery of as much light as possible through the DeltaRAM™ X random access monochromator. The PowerArc lamp housing is powered by a simple push button, ignition safe, integrated power supply with a lamp usage meter. Patented DeltaRAM™ X Random Access Monochromator The heart of the DeltaRAM™ X illuminator is the Random Access Monochromator. The DeltaRAM™ X monochromator is a scanning monochromator that delivers light anywhere from 250 to 650 nm. The monochromator uses a grating to disperse the different wavelengths of light from the incident white light xenon lamp. The scanning element inside the monochromator is a galvanometer which can switch between wavelengths in 2 milliseconds. With a simple and direct voltage control the DeltaRAM monochromator can hop around to multiple wavelength pairs much like a filter wheel. The DeltaRAM™ X however is much faster. It can be continuously tuned to any precise wavelength from 260 to 650 nm and it has an adjustable bandwidth. It can even be scanned like a traditional monochromator to provide spectra.The DeltaRAM™ X has a continuously adjustable bandwidth because there is no best bandwidth to do any particular fluorescence experiment. Some users prefer to keep the bandwidths as narrow as possible while still getting good throughput because a narrower slit will have less photobleaching. Some experiments however require opening up the slits to maximize speed or signal to noise. With ratiometric experiments such as with Fura-2 or BCECF you want to be sure that the bandwidth is not so large that you will get cross talk between the two excitation channels. For this reason the DeltaRAM™ X lets you empirically test and precisely set the best bandwidths for you. The bandwidth is continuously adjustable from 0 to 24 nm with a manual slit adjustment. DeltaRAM™ X Wavelength ControlThe DeltaRAM™ X is operated remotely, so it requires an external interface, or DAC, that is run by software. To control the DeltaRAM™ X wavelength position you need an interface device that provides a voltage to a BNC connector labeled “position” on the unit. The wavelength position is linear with respect to applied voltage as follows.DeltaRAM Position:Applied VoltageCorresponding Wavelength4.87 volts 250 nm0 volts450 nm-4.87 volts650 nm DeltaRAM™ X Shutter ControlThere is a BNC connector labeled “shutter” to digitally open and close the shutter. This is a simple solenoid shutter that is not intended for high speed shuttering. DeltaRAM™ X DAC Interface OptionsPCI DAC:The DeltaRAM™ X can be purchased with an optional 16 bit PCI DAC board that is slotted into a computer. A PCI DAC is necessary for millisecond wavelength switching.USB DAC:OBB also offers two low cost optional USB DAC interfaces for slower wavelength switching requirements. One is based on a National Instruments driver and the other uses a Measurements Computing driver.The OBB USB DAC using the National Instruments driver converts input commands from 0 to +5 volts into +4.87 to -4.87 volts on the Analog Out 1 BNC. As such, input commands from 0 to 5 volts drive the DeltaRAM™ X from 250 to 650 nm. This interface includes BNC connectors for Shutter 1 and 2, Trigger A and B, Analog Out 1 (DeltaRAM™ X Position) and 2. The OBB USB DAC using the Measurements Computing driver converts input commands from 0 to +5 volts into +4.87 to -4.87 volts on the Analog Out 1 BNC. As such, input commands from 0 to 5 volts drive the DeltaRAM™ X from 250 to 650 nm. This interface includes BNC connectors for Shutter Out, TTL In, TTL Out and Analog Out (DeltaRAM™ X Position)OBB DAC Software:ALL OBB DAC interfaces come with a LabVIEW driver and a simple USB 1.1 compatible interface with a very basic control software package that is Windows2000/XP compliant. This simple program will allow you to set and move wavelengths and manually open and close the shutter.SpecificationsExcitation Wavelength Range250–650 nm Wavelength Selection Speed 2 millisecondsBeam Uniformity 5%Stray-light rejection 10-4Wavelength Accuracy+/- 1 nmOptical Output 15 mW of optical outputrif line-height: 20px "

品牌：卡尔蔡司型号：Axio Scan.Z1制造商：德国卡尔蔡司公司经销商：北京普瑞赛司仪器有限公司产地：德国概述： 用Axio Scan.Z1数码化您的样本——以可靠的、可重复的方式创建高质量的数字玻片。优异的产品性能保证能够获取高品质的图像——正如您对于蔡司优质产品所期待的。即使是以前所未有的速度采集的荧光图像，依旧能够保持极高的图像质量。特点 最多达 9 个荧光通道 三个同步高速运转的滤光轮用于激发、分光和光发射，可在 40 毫秒以内完成通道切换 高灵敏度摄像头、高校准的光学系统和完美光源，确保了虚拟切片卓越的图像质量和拍摄时极短的曝光时间 Colibri.2 LED 光源光照柔和，将样本的损伤降至更低 可以在毫秒内转换激发的波长 当使用三通或四通滤片时，光路中不会有任何机械部件的移动 自动校准，从几何校准一直到色差校准，因此数字虚拟玻片图像可以在任何时间精确的重现 虚拟切片的数量、检测模式和相机种类可以根据需求对其进行升级 模块化托盘设计 – 该设计提供在选择虚拟玻片时最大限度的自由，可以使用 26 mm x 76 mm 和 52 mm x 76 mm 两种规格的玻片 自动化的操作流程和实时监测设计，使您在实验中可以轻松调节所有的设置 自动保存虚拟切片的元数据，您能通过点击一个按钮轻松恢复到数据处理前的状态 应用：蔡司 Axio Scan.Z1 能为您的应用创建品质卓越的虚拟玻片。在许多虚拟显微应用中提供出色的成像结果，其中包括： 阿尔茨海默氏症的发病机理 – 提供高分辨率的虚拟玻片的明场图像并进行分析，帮助您建立转基因小鼠对淀粉样沉积物的分析模型 癌症研究 – 使用虚拟显微技术以出色的荧光虚拟切片来研究癌症发病的根本 毒药动力学/毒理学 – 自动校准、图像分析和远程阅览等功能可以实现自动化的成像分析流程以及远程联网进行评议 荧光原位杂交（FISH） – 在基因组中使用多通道荧光和景深延伸来探知在染色体组中单个序列的拷贝数量 目标的识别与特征描述 – 使用灵敏的荧光成像结合正确的样品处理来标识和鉴定药物激活底物搜寻的靶标 同种异体移植物和异种移植物的免疫反应 – 使用虚拟显微技术的高动态范围和绝对真实的图像来识别特定细胞的表型并了解组织内细胞的相互反 神经外伤领域的研究 – 使用虚拟切片系统的Z轴光切成像和高通量成像对大脑损伤区域进行量化测量 组织芯片技术（TMA） – 可靠的样品检测和稳定的扫描过程，有效的利用试剂以及样品组织 生物医学领域的合作研究 – 灵活配置的虚拟扫描成像系统，利用了“托盘式”设计概念，作为无标准可循的样品托架 ZEN slidescan：多用途且易于使用的虚拟显微成像软件Axio Scan.Z1 的 ZEN 成像软件是基于直观的用户思维理念而设计，可以根据掌握的知识和职责来给用户和用户群定义功能。ZEN会根据您做出的设置做出回应，紧接着做出正确的行动。例如：智能光路设置，会给您提供一个直观的界面和工作流程，即使是复杂的荧光样本实验也一样解决。只需选择染料，ZEN 软件会自动制定好其他的设置。去卷积能生成更加透明干净的光学切片图像，消除了焦平面以外的杂散光。Axio Scan.Z1 基于 ZEN Lite 软件，包括编辑和浏览虚拟切片图像的功能。您可以使用总览图导航到感兴趣的区域，追踪过去任何看过的地方。使用圆形、矩形、线条、箭头、文本、轮廓图等对图像进行标注。比较多达 16幅图像，并且可以将它们联系在一起进行导航观察，例如：创建一种直接比较连续切片的方式。可以处理蔡司特有的 CZI 文件格式，也可以处理 TIFF、JPEG、PNG 和 GIF 格式的图片，实现更大兼容性及共享虚拟显微图像。从视图菜单中或图库中选择 2D 模式和直方图模式。您还可以对单个或批量图像进行锐化、模糊、几何函数和颜色调整处理。使用 ZEN lite，可以 执行简单交互式测量，并且可以输出测量结果，例如：长度、面积和角度。ZEN lite 还有工作区域大小可调的功能，软件本身具有暗色和亮色两种不同的显示模式，用户可自由选择。肾切片，4 μm，PAS（对氨基水杨酸反应）对肾小球进行染色。资料由美国匹兹堡大学病理部门的Kumiko Isse 教授以及美国移植研究所 的Thomas E. Starzl 提供。 小鼠大脑，5 μm，冷冻切片，量子点标记：DAPI，CD31（内皮素细胞素），胶质纤维酸性蛋白（星形胶质细胞），资料由美国匹兹堡大学病理学部门的 Kumiko Isse 教授以及美国移植研究所 的Thomas E. Starzl 提供。结肠组织，4 μm，量子点标记：DAPI、CD56、CD3、CD68 以及CD20，资料由美国匹兹堡大学病理学部分的Kumiko Isse 教授以及美国移植研究所的 Thomas E. Starzl 提供。技术解决方案蔡司 Axio Scan.Z1可升级的模块化设计，根据需求灵活配置。该系统可以支持高达50个尺寸为28 mm x 48 mm的薄片（可同时容纳100个1”×3”的标准显微薄片）。每一个薄片都可以配备一个条形码（一维码或二维码），用于识别和归档数字化的薄片。得益于先进的蔡司托盘理念和极其可靠的组件，蔡司 Axio Scan.Z1为用户提供一个强大的扫描过程。托盘中的薄片样品送入弹仓中即可完成检测，因此该系统不易受样品制备的影响，如盖薄片，薄片边缘的破损等，因为移动的是薄片所定义的托盘，而不是其真实的组织结构。该系统接受大尺寸公差的薄片（包括美国及ISO的标准），如Giessener格式，1” ×3”和2×3”的薄片。也可以定制其他薄片尺寸的托盘。蔡司 Axio Scan.Z1的宏观相机用于对样品的概览和导航。该系统提供了两组相机成像，以获得效果最好的明场、偏光（拥有完美的色彩还原和高分辨率的3芯片摄像头）和荧光图像（如高灵敏度和快速扫描的Orca闪光灯呈像）。样品可以实现明场、偏光和荧光/环孔对比。蔡司 Axio Scan.Z1包含一个内置的用于透射光的LED照明光源，同时可以配备一个额外的用于荧光照明的Colibri2和/或HXP 120。因此，在明场或偏光通道可以结合另外的荧光通道。蔡司 Axio Scan.Z1的图像采集系统是基于ZEN blue成像软件的概念，同时包含ZEN用于图像分析和开放应用开发（OAD）的集成模块。通过ZEN浏览器提供的一个基于网络的数据管理和远程浏览，使得与世界各地的其他专家分享他们归档的图像成为可能。蔡司 Axio Scan.Z1带有几何校准功能，能够获得合适的标尺，同时针对不同物镜具有精确的齐焦性及合轴性，提供可靠的图像质量。另外可选配一个特殊的颜色校准片以确保白平衡和输出颜色一致。（以20×物镜为例）当薄片样品尺寸为15×15 mm2，以0.22 μm / pixel的速度扫描时，得到薄片的概览图和明场图像的时间大约是4分钟。同一条件下得到偏光图像的时间大约是12分钟。 灵活多样的组件1 显微镜? Axio Scan.Z1? 玻片进样系统（可选择12张或100张的配置）? 托盘式样品夹（可适用于三种玻片规格： 26mm x 76mm，52mm x76mm，或102mm x 76mm）2 物镜? N-Achroplan (2.5x, 10x, 20x, 40x)? Fluar (2.5x, 5x)? Plan-Apochromat (10x, 20x, 40x)3 照明? 透射光光源：LED (波长范围400~700nm，最高亮度在460nm）? 荧光光源：365nm，385nm，420nm，445nm,470nm, 505nm, 530nm, 590nm,615nm, 625nm或者540~580nm， HXP120 V? 滤片转盘：10位带ACR(自动组件识别功能)的滤片或6位高速激发滤片转盘6位高速分光滤片转盘6位高速发射滤片转盘54 相机? Hitachi HV F202? AxioCam MRm? Hamamatsu Orca Flash 4.01 2 3 45 软件? ZEN slidescan? ZEN lite? ZEN 2 （Blue edition)? ZEN browser

GDS-50型秒表检定仪市面上其他厂家的秒表检定仪功能含秒表检定仪、指针式电秒表检定仪、数字式电秒表检定仪、毫秒表检定仪四合一功能只能检定秒表测试对象对比可检：401/405电秒表407/408电秒表411数字式毫秒计415/417/417B型数字式电秒表只能检普通秒表秒表测试范围300ms~9999999999s1-99999s指针式电秒表测试范围0.02s~9999999999s0.1s-99999s数字式电秒表/毫秒表测试范围0.1us~9999999999s1×102μs～1×109μs显示液晶屏数码管外观超薄款式较老GDS-50秒表检定仪是一新用的时间检定仪，供各级计量部门，工厂，院校及科研单位检定各种秒表、毫秒仪，机械秒表等，性能符合检定规程的要求。 技术指标： 1、 检定电子秒表和机械秒表： 在1－99999秒内可任意置数 准确度±（2＊10－7＊输出时段）秒±0.003秒 2、 检定电子毫秒仪（正负两组输出） 在1－99999秒内可任意置数，最小输出100微秒 准确度±（2＊10－7＊输出时段）秒±0.003秒 3、 检定电秒表（适合新规程JJG237-95） 在1－99999秒内可 任意置数 指针式准确度：电同步＜±0.6毫秒 数字式准确度：±（2＊10－7＊输出时段）秒±0.006秒 IntroductionThe calibrator is a precision instrument calibrating mechanical stopwatch, electronic stopwatch, and 405/407/408/415/417 type pointer electric stopwatch. The SF2001 stopwatch calibrator is compliant with the stopwatch calibration procedure JJG 237-2010 issued by the National Metrological Bureau. It could be used at metrological department at all levels, factory, institutions and all research institutes to calibrate electric stopwatch (405,407,408,415/417), mechanical stopwatch and digital quartz electronic stopwatch. The instrument is fully digitally controlled, high reliability,high accuracy, complete functions, convenient operation, and strong anti-interfere ability. GDS-50 stopwatch calibrator and its accessories: 1)GDS-50 stopwatch calibrator 1 piece 2) Three-core power cord 1 piece 3) Dual banana plug test lead 2 pieces 4) BNC-banana test lead 1 piece 5) Soft copy of User’s Guide 1 piece