-
精彩回放
成像流式作为独特的流式技术,因其超高灵敏度和可视化的流式数据,突破了长期以来存在于外泌体、纳米材料等微颗粒检测中的瓶颈。通过提供明场和多通道的荧光图像,清晰分辨每个微颗粒的表征,溯源其与亲源细胞间的关系,以及与靶向细胞间的相互作用,在微颗粒研究、体外诊断、药物开发等领域有着独特的、不可或缺的应用。
-
精彩回放
报告首先简单介绍上海交通大学生命科学技术学院仪器共享与技术服务平台的流式细胞仪,然后介绍微生物流式分析参数设置等问题,最后重点介绍微生物的流式分析和分选案例。
-
精彩回放
一、流式诊断淋巴瘤基础
1、异常表达
2、单克隆评价
3、免疫分型三部曲
二、临床常见问题:
1、MICMP只有流式阳性,能否诊断淋巴瘤?
2、反应性?肿瘤性?
3、一元论?两元论?
-
精彩回放
Objective: Hepatocellular carcinoma (HCC) tumour microenvironment (TME) is highly complex with diverse cellular components organizing into various functional units (CN). And we wanted to define CN of HCC while preserving the TME architecture, based on which, potential targets for novel immunotherapy could be identified.
Design: A highly multiplexed imaging mass cytometry (IMC) panel was designed to simultaneously quantify 36 biomarkers of tissues from 134 HCC patients and 7 healthy donors to generate 562 highly multiplexed histology images at single-cell resolution. Different function units were defined by topological analysis of TME. CN relevant to the patients’ prognosis was identified as specific target for HCC therapy. Transgenic mouse models were used to validate the novel immunotherapy target for HCC.
Results: Three major types of intra-tumour areas with distinct distribution patterns of tumoral, stromal and immune cells were identified. 22 cellular metaclusters and 16 CN were defined. CN composed of various types of cells formed regional function units and the regional immunity was regulated reversely by resident Kupffer cells and infiltrating macrophages with pro- and anti-tumour function, respectively. Depletion of Kupffer cells in mouse liver largely enhances the T cell response, reduces liver tumour growth, and sensitizes the tumour response to anti-PD-1 treatment.
Conclusion: Our findings reveal for the first time the various topological function units of HCC TME, which also presents the largest depository of pathological landscape for HCC. This work highlights the potential of Kupffer specific targeting rather than overall myeloid cell blocking as a novel immunotherapy for HCC treatment.
-
精彩回放
Quality cytometric data generation is dependent on undertaking the multi-factor processes involved in experimental setup, panel design, sample processing, acquisition, data analysis and interpretation correctly. These tips and tricks to be presented are relevant across the workflow and are presented to remind users of the occasionally overlooked basic and advanced processes to generate quality data and interpretation.
-
精彩回放
Compared to conventional compensation, spectral unmixing distinguishes the shapes of emission spectra along a large range of wave lengths. This approach can discriminate fluorochromes of similar emission spectrum, as well as provide better separation. Although the measurement of a continuous wave length is impossible in conventional cytometry, a high parameter cytometer can well cover most of the light spectrum by enabling all detectors. This provides a “pseudo-spectral approach” that can provide better signal separation than conventional compensation in theory.
This presentation will discuss how high parameter data may benefit from utilizing pseudo spectral unmixing instead of normal compensation. Procedures for spectral unmixing on FlowJo V10.6 will also be covered.
-
精彩回放
仪器的质控是流式课题必须和不可或缺的步骤。流式仪每天都需要通过质控以确保数据的准确性。这个讲座将会讨论日常流式仪器质控的主要参数,如何进行和解读质控,仪器性能跟踪和故障排除。还将讨论长期流式课题的实验间质控的必须性和方法,并且分享如何通过质控获得可重复性的结果。
-
精彩回放
病毒是颗粒很小、以纳米为测量单位的非细胞型微生物。它在疾病预防、传染病爆发等方面扮演重要角色。对病毒生物学、病毒与宿主相互作用、免疫应答机制等研究,有利于药物与疫苗的研发,建立快速有效的临床诊断和治疗体系。流式细胞术在病毒颗粒检测、疫苗效价评估、免疫细胞亚群及免疫反应分析等流程中是必不可少的方法之一。
-
精彩回放
围绕水环境保护、渔业可持续发展、微藻生物能源方面的重大战略需求,以本所典型案例,从细胞水平、分子水平等多个层面全面剖析流式细胞技术在鱼类遗传育种学、藻类生物学、淡水生态学、水环境工程学和保护生物学的应用策略与技术优势。
-
精彩回放
流式荧光技术的原理特点,国内外细胞因子产品汇总比较,细胞因子开发过程中遇到问题