This webcast will present recently-developed approaches, ranging from sample preparation and chromatographic separation to advanced data acquisition, data interpretation and visualization methods. Advances in instrumentation, including the novel Orbitrap Astral MS, and how to use both TMTPro-labeled and label-free approaches, will be presented, showcasing the application of these methods to biomedical questions, and providing an overview of the current technological state of the field. Learn how to: 1.Prepare single cells for analysis by mass spectrometry； 2.Improve the sensitivity and throughput of single-cell proteomics analysis； 3.Implement both label-free and labeled quantitative approaches to single-cell proteomics.