Liquid extraction surface analysis (LESA) is an ambient surface mass spectrometry approach which offers great potential for protein analysis. The benefits of LESA are speed of analysis, reduced sample preparation requirements and sample loss, potential for multiple sampling of the same location and, in the case of tissue sections, the ability to image multiple analytes. We have previously demonstrated LESA mass spectrometry of proteins from dried blood spots, thin tissue sections and bacteria growing on agar. Here, we present the latest developments in LESA mass spectrometry and mass spectrometry imaging, including native mass spectrometry imaging, of proteins directly from thin tissue sections.