Dionex BorateTrap 硼酸盐捕获柱
对于使用HPAE-PAD检测IC碳水化合物分析中最佳的性能,使用的Thermo Scientific戴安BorateTrap在线捕获柱除去硼酸盐污染。当放置洗脱液泵和注射阀,陷阱上的碳水化合物分析物的效率或保留时间没有影响之间。的戴安BorateTrap柱消除了甘露糖,果糖,以及从硼酸污染引起糖醇峰拖尾。
Dionex BorateTrap 硼酸盐捕获柱
订货信息:
The BorateTrap™ is an in-line trap column, and is recommended for
optimal performance during carbohydrate analysis.
In-line trap column removes borate contamination for HPAE-PAD.
Eliminates peak tailing for mannose, fructose, and sugar alcohols resulting
from borate contamination.
Borate contamination in eluents can cause a significant loss of peak
efficiency, especially for mannose, fructose, and reduced monosaccharides. If
borate is present in the eluent, it binds both to the anion-exchange column and
to the carbohydrate analytes. The carbohydrate-borate complex is less
efficiently eluted from the anion-exchanger than is the carbohydrate, resulting
in peak tailing, particularly where vicinal cis hydroxyl groups are present,
such as for mannose and sugar alcohols. The BorateTrap eliminates peak tailing
for mannose, fructose, and reduced monosaccharides, resulting from contamination
of the eluent from borate. Therefore, the BorateTrap is highly recommended for
optimal performance during carbohydrate analysis.
The BorateTrap is a 4 × 50 mm column placed between the eluent pump and the
injection valve to remove borate contamination from eluents used for
HPAE-PAD. Because it is placed before the injection valve, it has no effect on
the efficiencies or retention times of the carbohydrate analytes. This column is
packed with a 20-μm high-capacity resin that has high selectivity for borate.
The resin used in the BorateTrap is functionalized with a polyol capable of
complexing borate in the presence of hydroxide. Borate is a known contaminant in
laboratory water supplies. In chromatography, borate contamination of HPLC
eluents may be a result of degrading deionized water systems or as leachate from
borosilicate glassware.
Effect of Borate and the BorateTrap Column on Monosaccharide Peak
Symmetry.
Data Sheets |
---|
| CarboPac
MA1 Column for Carbohydrate Alditol and Aldose Analysis Data Sheet |
| CarboPac
PA1 and PA10 Columns for Mono- and Disaccharide Analysis Data
Sheet |
| CarboPac
PA20 Column Data Sheet |
Manuals |
---|
| BorateTrap
Column Manual |
| CarboPac
MA1, PA1, PA10, and PA100 Analytical Columns Manual |
| CarboPac
PA20 Analytical Column Manual |
Technical Notes |
---|
| TN
40: Glycoprotein Monosaccharide Analysis Using HPAE-PAD with Eluent
Generation |
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