Acclaim 300 C18 蛋白和肽柱
使用 Thermo Scientific™ Acclaim™ 300 C18 液相色谱柱,可进行高分离度肽图分析和蛋白质分离。 3μm 超纯硅胶颗粒可降低分析物进入固定相的扩散限值,从而可快速进行分离度分离。 与 5μm 色谱柱填料相比,较小的颗粒支持在更短的色谱柱中增加流速并采用更浅的梯度,从而加快分离速度。
描述
高分辨率分离蛋白质和肽
300? 微孔硅胶上的高性能 C18 化学键合
经过应用测试,适用于肽图分析
重现性好,结果可靠
流失率极低,与质谱完全兼容
在 pH 值 2.5 - 7.5 之间保持稳定
关于 Acclaim HPLC 色谱柱系列
使用 Thermo Scientific™ Acclaim™ PepMap™ 100 C18 液相色谱柱对胰蛋白酶肽、天然肽和合成肽进行高分辨率肽图分析。 这些色谱柱通常用于蛋白鉴定、生物标记物发现和系统生物学的 LC-MS/MS 肽图分析。 Acclaim PepMap 100 C18 色谱柱载样能力高,因此非常适合分析复杂蛋白组学样品中的低丰度肽。 大多数 C18 色谱柱都预先组装了 Thermo Scientific™ nanoViper™ 手拧接头,可实现轻松安装。 另外也可提供传统色谱柱。
描述
为蛋白质鉴定、生物标记发现和系统生物学提供高分辨率
其特定的载荷能力可在 LC/MS 中实现最高灵敏度
设计用于无 TFA 的 LC-MS,最大限度减少离子抑制作用
为接入 ESI/MS 和 MALDI-MS 进行过优化
柱间重现性最高
使用方便,尖端微型化 HPLC
预装 nanoViper 柱入口接头,便于安装色谱柱
nanoViper 接头的主要优点
最适合对胰蛋白酶肽、天然肽和合成肽进行高分辨率肽图分析,以及进行关于蛋白质鉴定、生物标记发现和系统生物学的 LC-MS/MS 肽图分析。
高载样量
用来分析经常存在于复杂蛋白质组学样品中的低丰度肽
根据通量、灵敏度和样品可用量,选择色谱柱的长度和内径。
可选择长度 50、150、250、500 mm,内径 50、75、300 和 1000 μm
卓越的蛋白和多肽分离。
不含三氟乙酸 (TFA) 的 LC/MS 为提高 MS 灵敏度,使抑制效应最小化
卓越的分离效率
卓越的分离度和回收率
使用 nanoViper™ 手旋接头轻松安装
PepMap 纳米色谱柱还可预装 Thermo Scientific™ Dionex™ nanoViper™ 手拧接头,以零死体积的状态连接至 1000 bar。
无需工具
实现几乎零死体积连接
承受压力最高可达 1000 bar
关于纳米、毛细和微型 Acclaim PepMap 色谱柱
纳米 (75 μm)、毛细 (300 μm) 和微型 (1000 μm) 色谱柱适用于蛋白和肽分析。 通常,对样品量有限制,较小内径的色谱柱可以在样品有限的情况下提高灵敏度。 当纳米 LC 色谱柱常用于蛋白组学时,毛细色谱柱和微型色谱柱的浓缩效应对代谢组学或推进 LC-MS 连接有益。 Thermo Scientific Acclaim PepMap 色谱柱在各类针对肽和蛋白分析的化学作用中提供纳米柱、毛细柱和微型柱。
在借助质谱检测功能进行液相色谱分离之前,可使用 PepMap Trap 色谱柱进行肽脱盐和预富集。 捕获柱可为一维肽图分析和二维液相色谱分析提供最高效率。
Acclaim 300 C18 蛋白和肽柱
订货信息:
分析柱 |
Acclaim 300, C18, 3 μm Analytical (2.1 x 50 mm) | 60263 |
Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm) | 60264 |
Acclaim 300, C18, 3 μm (3.0 x 150 mm) | 63684 |
Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm) | 60265 |
Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm) | 60266 |
保护柱 |
Acclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580 | 69690 |
PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA | 69697 |
Hardware |
Acclaim SST Guard Cartridge Holder V-1 | 59456 |
Guard to Analytical Column Coupler | 59457 |
Guard Kit (Holder and Coupler) | 59526 |
Acclaim SST Guard Cartridge Holder V-2 | 69580 |
Acclaim Guard Kit (Holder and coupler) V-2 | 69707 |
Guard to Analytical Column Coupler V-2 | 74188 |
Micro and Nano Columns |
Acclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper | 164567 |
75 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper | 164708 |
300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper | 164716 |
300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper | 164718 |
Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm | 163584 |
Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper | 164572 |
High-Resolution Separation of Proteins and Peptides
The Acclaim® 300 features 3 μm silica particles for rapid analysis
of complex protein digests. The Acclaim 300’s stable bonding make these columns
compatible with a wide variety of applications, including LC/MS methods.
Designed for high-resolution peptide mapping and protein separations
High-efficiency 3 μm spherical silica substrate
High-performance bonding chemistry on 300 ? pore silica
Application tested for suitablility in peptide mapping
Reproducible for dependable results
LC/MS compatible
Manufacture of these columns starts with ultrapure silica that contains
minimal concentrations of metal contaminants, minimizing the tailing effects of
residual, exposed silanol groups. Exhaustive bonding and end-capping techniques
result in stable bonding and columns that exhibit predictable reversed-phase
separations with minimal secondary interactions.
The use of a 3 μm silica particle reduces limits for diffusion of the
analytes into the stationary phase, resulting in fast, high-resolution
separations. Compared to 5 μm column packings, the smaller particles support
increased flow rates and shallower gradients on shorter columns, for faster
separations. Dionex tests each bonded silica lot for peptide selectivity to
ensure reproducible performance.
Acclaim PepMap for TFA-Free Peptide Mapping
Specifications |
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Column type | Nano | Capillary | Micro |
Column i.d. | 75 μm | 300 μm | 1.0 mm |
Length | 50, 150, 250, and 500 mm | 50, 150, and 250 mm | 50, 150, and 250 mm |
Recommended Flow Rate | 200 nL/min | 4 μL/min | 40 μL/min |
Packing Material | C18, 100 ?, 3 μm | C18, 100 ?, 3 μm | C18, 100 ?, 3 μm |
C18, 300 ?, 5 μm | C18, 300 ?, 5 μm | C18, 300 ?, 5 μm |
C8, 100 ?, 3 μm | C8, 100 ?, 3 μm | C8, 100 ?, 3 μm |
C8, 100 ?, 5 μm | C8, 100 ?, 5 μm | C8, 100 ?, 5 μm |
C4, 300 ?, 5 μm | C4, 300 ?, 5 μm | C4, 300 ?, 5 μm |
Column | Fused-silica | Glass Lined Tubing | Stainless steel |
Column Inlet | 1/16-in. PEEK sleeve adapter (provided) nanoViper (optional) | 1/16-in. universal fitting nanoViper (optional) 50μm i.d. PEEK
sheathed fused silica with nanoViper fittings | 1/16-in. PEEK tubing nanoViper (optional) 75μm i.d. PEEK sheathed
fused silica with nanoViper fittings |
Column Outlet | Fused-silica 25-μm i.d./ 280-μm o.d. | Fused-silica 75-μm i.d./ 280-μm o.d | 1/16-in. PEEK tubing nanoViper (optional) 75μm i.d. PEEK sheathed
fused silica with nanoViper fittings |
MS Outlet | 1-m Length |
![](http://www.dionex.com/en-us/images/page-cgram/img-76618-b-20395-Cytochrome-c-Tryptic-Digest.jpg)
Cytochrome c tryptic digest.
![](http://www.dionex.com/en-us/images/page-cgram/img-76664-b-23064-Acid-Additives-Peptide-Analysis-Acclaim-300.jpg)
Comparison of acid additives for peptide analysis.
![](http://www.dionex.com/en-us/images/page-cgram/img-76623-b-20408-Capillary-LC-Peptide-Mapping-Acclaim-300-C18.jpg)
Capillary LC for peptide mapping.
![](http://www.dionex.com/en-us/images/page-cgram/img-76622-b-20407-Protein-Rev-Phase-HPLC-Acclaim-300-C18.jpg)
Protein mixture separated using reversed-phase HPLC.
Brochures |
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![](http://www.dionex.com/assets/images/pdficonsm.gif) | Catalog:
Acclaim Bonded Silica-Based Columns for HPLC |
Product Data Sheets |
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![](http://www.dionex.com/assets/images/pdficonsm.gif) | Acclaim 300 HPLC
Columns Data Sheet |
Application Notes |
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![](http://www.dionex.com/assets/images/pdficonsm.gif) | AN 1055:
Determination of Virginiamycin, Erythromycin, and Penicillin in Dried Distillers
Grains with Solubles |
![](http://www.dionex.com/assets/images/pdficonsm.gif) | AN 230:
Purity Analysis of Synthetic Thymosin Alpha 1 by Reversed-Phase HPLC with an
Acclaim 300 C18 Column |
Application Updates |
---|
![](http://www.dionex.com/assets/images/pdficonsm.gif) | AU 156:
Evaluation of Acclaim HPLC Columns Using the National Institute of Standards
Standard Reference Material 870 |
![](http://www.dionex.com/assets/images/pdficonsm.gif) | AU
183: Separation of Peptides from Enzymatic Digestion on Different Acclaim
Columns: A Comparative Study |
Technical Notes |
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![](http://www.dionex.com/assets/images/pdficonsm.gif) | TN 705:
Automated Enrichment and Determination of Phosphopeptides Using Immobilized
Metal Affinity and Reversed-Phase Chromatography with Column
Switching |
Guides |
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![](http://www.dionex.com/assets/images/pdficonsm.gif) | Acclaim Care
and Handling Instructions |
Acclaim 300 C18 蛋白和肽柱信息由北京豫维科技有限公司为您提供,如您想了解更多关于Acclaim 300 C18 蛋白和肽柱报价、型号、参数等信息,欢迎来电或留言咨询。除供应Acclaim 300 C18 蛋白和肽柱外,北京豫维科技有限公司还可为您提供Vela DIOL 液相色谱柱、Vela C30 液相色谱柱 、豫维 YW-DD2.5T/YW-DD10T型氘灯等产品,公司有专业的客户服务团队,是您值得信赖的合作伙伴。