CarboPac MA1 糖类分析柱
用途和特点:
CarboPac MA1柱适用于食品和体液中还原性单糖和二糖的分析。无需衍生,室温进行,灵敏度远高于示差折光检测。
CarboPac MA1 糖类分析柱
订货信息:
分析柱 |
CarboPac MA1 Analytical Column (4 x 250 mm) | 044066 |
保护柱 |
CarboPac MA1 Guard Column (4 x 50 mm) | 044067 |
CarboPac MA1 Column for Carbohydrate, Alditol, and Aldose Analysis
The CarboPac? MA1 column is a high-capacity, strong anion-exchange
column that delivers sensitive, rugged, and reliable separations of reduced
sugars without the need for derivatization. The MA1 consists of a macroporous
resin fully functionalized with a tertiary amine.
Recommended for the separation of reduced mono- and disaccharides
No derivatization needed
Separations using a simple hydroxide eluent
Alditols and aldoses separated from one another in a single run
Designed for reduced mono- and disaccharide alditol analyses, the CarboPac
MA1 is also well suited for analysis of exoglycosidase-released neutral
monosaccharides. Monosaccharides are moved away from the column void, enabling
immediate evaluation of contaminating exoglycosidase activities. The MA1 can
provide baseline resolution of fucose, N-acetyl-(D)-glucosamine,
N-acetyl-galactosamine, mannose, glucose, and galactose, as well as neutral
oligosaccharides in the same separation.
CarboPac MA1 Column for Carbohydrate Alditol and Aldose Analysis
Specifications |
---|
Dimensions | CarboPac MA1 Analytical Column: 4 × 250 mm CarboPac MA1 Guard
Column: 4 × 50 mm |
Maximum operating pressure | 2000 psi (14 MPa) |
Temperature range | 4–50 °C Recommended operating temperature: ambient |
Typical operating conditions | Recommended flow rate: 0.2–0.5 mL/min |
Mobile phase compatibility | pH 0–14; incompatible with solvents and anionic detergents. Hydroxide
eluents only. |
Resin composition | 7.5-μm-diameter vinylbenzyl chloride/divinylbenzene macroporous substrate
fully functionalized with an alkyl quaternary ammonium group (15%
cross-linked). |
Anion-exchange capacity | Approximately 1450 μeq/column (4 × 250 mm analytical column) |
Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are
nonmetallic. |
Gradient separation of alditols and aldoses potentially released by
hydrolysis of beta eliminated oligosaccharides. Arabitol serves as an internal
standard.
HPAE separation of sugars and sugar alcohols without the need for
derivatization.
Isocratic separation of a representative set of alditols and aldoses ensures
lot-to-lot reproducibility in the manufacturing of the CarboPac MA1
column.
Manuals |
---|
| Dionex
CarboPac MA1 Column Product Manaul |
Product Data Sheets |
---|
| CarboPac
MA1 Column for Carbohydrate Alditol and Aldose Analysis Data
Sheet |
Application Notes |
---|
| AN:
267 Analysis of the Aminoglycoside Antibiotics Kanamycin and Amikacin Matches
USP Requirements |
| AN
246: Determination of Ethylene Glycol and Diethylene Glycol in a Sorbitol
Solution |
| AN
117: Quantification of Carbohydrates and Glycols in
Pharmaceuticals |
| AN
122: The Determination of Carbohydrates, Alcohols, and Glycols in Fermentation
Broths |
| AN
87: Determination of Sugar Alcohols in Confections and Fruit Juices by
HPAE-PAD |
Technical Notes |
---|
| TN
21: Optimal Settings for Pulsed Amperometric Detection of Carbohydrates Using
the Dionex ED40 Electrochemical Detector |
| TN
20: Analysis of Carbohydrates by High Performance Anion Exchange Chromatography
with Pulsed Amperometric Detection (HPAE-PAD) |
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