Dionex AminoPac PA10 氨基酸分析柱

Dionex AminoPac PA10 氨基酸分析柱

用途和特点
AminoPac PA10柱结合阴离子交换，梯度淋洗以及脉冲电化学检测，可以分离多种氨基酸。分离氨基酸的同时，还可检测氨基糖及碳水化合物。此外，还可分离多种糖类，磷酸化氨基酸，以及含硫氨基酸的普通氧化产物，如磺基丙氨酸。其柱填料为疏水性高聚物薄壳型阴离子交换剂，可在pH0-14范围内使用。填料独特的pH稳定性允许使用的淋洗液，有助于氨基酸在金电极的阳极氧化。

Dionex AminoPac PA10 氨基酸分析柱

订货信息：

For High-Resolution Separations of Free Amino Acids by AAA-Direct

The AminoPac^® PA10 column is packed with a hydrophobic, polymeric, pellicular, anion-exchange resin that is stable over the range of pH 0–14. The unique pH stability allows the use of eluents that are conducive to anodic oxidation of amino acids at gold electrodes. This column is recommended for use with the AAA-Direct^™ Amino Acid Analyzer, allowing direct detection of primary and secondary amino acids by IPAD, with no need for pre- or postcolumn derivatization.

• Derivatization not required

• Carbohydrates and amino acids separated and detected simultaneously

• Mid-femtomole to low-picomole detection limits

• Compatible with all commonly used hydrolysis procedures

• Can be configured for on-line analysis

AAA-Direct is a technique that eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths. 

The AminoPac PA10 column is optimized for separations of free amino acids by AAA-Direct. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.

Determination of amino acids and carbohydrates in LB broth supernatant.

Amino acids and sugars in red wine.

"High Glucose" Fermentation Broth (1:250 Dilution)

Separation of an Amino Acid Standard Mixture Containing 100 pmol of Each Component.

Comparison of Amino Acid Concentrations for Collagen Measured by Integrated Amperometry and Ninhydrin Methods.

Application Notes
	AN 179: Carbohydrate and Amino Acid Analysis Using 3-D Amperometry
	AN 150: Determination of Amino Acids in Cell Cultures and Fermentation Broths
	AN 142: Determination of Tryptophan Using AAA-Direct
	AN 130: Identification of a Hydroxylysine-Containing Peptide Using AAA-Direct

Technical Notes
	TN 55: Screening of Sample Matrices and Individual Matrix Ingredients for Suitability in AAA-Direct
	TN 50: Determination of the Amino Acid Content of Peptides by AAA-Direct

Guides
	AminoPac PA10 Analytical Column AAA
	AAA Direct System Installation Guide