Typical buffers: | Mobile phase formula 1 Mobile phase A: 2 M ammonium sulfate, 100 mM sodium
phosphate, pH 7.0 Mobile phase B: 100 mM sodium phosphate, pH 7.0
Mobile phase formula 2 Mobile phase A: 1.5 M ammonium sulfate, 50 mM
sodium phosphate, pH 7.0 / isopropanol (95:5 v/v) Mobile phase B: 50 mM
sodium phosphate, pH 7.0 / isopropanol (80:20 v/v) |