MAbPac Protein A LC 色谱柱

MAbPac Protein A LC 色谱柱

Thermo Scientific? MAbPac? Protein A LC 色谱柱用于收集细胞培养物，可以实现快速而准确的单克隆抗体 (MAb) 滴度分析。 该色谱柱将低反压和高效的优点相结合，可以实现高通量和高准确性的分析。 独特的规格使其适用于生物相容系统，可以快速而便捷的实现加载、结合、淋洗和收集自动化。 该色谱柱为非多孔、聚合物树脂，具有疏水的二乙烯基苯核及亲水表面，专为亲和分离而优化，具有出色的稳健性和可重现性。

描述

快速而准确的 MAb 滴度分析

• 完整循环时间（包括平衡）仅 2 分钟

• 较宽的浓度线性范围 (0.025 - 5 mg/mL)，无残留效应

• 柱压低，在 2mL/min 流速下柱压低于 250 psi

• 出色的样品回收率

MAbPac Protein A LC 色谱柱可用于滴度分析或浓缩与纯化 IgG 材料，以供后续实验或二维 (2D) 色谱分析使用。 该色谱柱规格为 HPLC 生物惰性、4×35mm PEEK 柱身。

MAbPac Protein A LC 色谱柱

订货信息：

MAbPac Protein A Column for Monoclonal Antibody Titer Analysis

The Thermo Scientific? MAbPac? Protein A column is a high-performance, affinity chromatography column specifically designed for fast, accurate determination of monoclonal antibody (MAb) concentration in the harvest cell culture (HCC). Early in the development of recombinant MAbs, a large of number of HCC samples must be screened for IgG titer. Affinity chromatography employing a Protein A ligand is often used to determine the MAb concentration. The MAbPac Protein A column is a high-pressure liquid chromatography (HPLC) column designed to provide fast, accurate, titer analysis with linearity over a wide concentration range without carryover.

The MAbPac Protein A column is based on a novel nonporous polymeric resin consisting of a hydrophobic divinylbenzene core and a hydrophilic surface. This proprietary resin is designed for fast mass transfer, resulting in efficient separations without carryover. The recombinant Protein A ligands are covalently attached onto the hydrophilic resin surface through their amine groups to provide a very rugged, reproducible column. The column is available in a HPLC bioinert, 4 × 35 mm PEEK column body.

Product Highlights: 

? A novel nonporous polymeric resin with a hydrophilic surface
? Functionalized with the recombinant protein A ligands
? Wide range of MAb quantitation: 0.025–5 mg/mL
? Fast analysis: 2 min/analysis
? Rugged
? Low pressure: column pressure is less than 250 psi at 2 mL/min

The MAbPac Protein A column can be used for both titer analysis or concentrating and purifying IgG material for later experiments or two-dimensional (2D) chromatography.

Data Sheets
	Thermo Scientific MAbPac Protein A Column

Manuals
	Thermo Scientific MAbPac Protein A Column Quickstart
	Thermo Scientific MAbPac Protein A Column Product Manual

Posters
	Protein A Affinity Column for Monoclonal Antibody (MAb) Titer Analysis

Applications
	A Novel Affinity Protein A Column for Monoclonal Antibody (MAb) Titer Analysis

Brochures
	Thermo Scientific BioLC Columns