Titansphere TiO 二氧化钛填料 5010-21315

Titansphere TiO 二氧化钛填料

Titansphere TiO填料，是将二氧化钛(TiO2)做为骨架结构的全新型HPLC用球状二氧化钛凝胶填充剂. 是唯一一款产业化了的二氧化钛凝胶色谱填料.以下为二氧化钛色谱填料。

Titansphere TiO填料是一种在碱条件下也同样能够使用的，与硅胶有着不同吸附特性的填充剂。其主要特点如下：
表面、形状平整的球状二氧化钛凝胶
表面积比较大
选择性保留磷酸化合物
优异的结构异构体分离能力
优异的耐酸、耐碱性

磷酸化肽的精制和浓缩

细胞中的磷酸化蛋白质，其存在量为微量且离子化效率低，因此质谱（MS）检测比较困难。但是，如果使用以二氧化钛（二氧化钛：TiO2）形成的均一多孔质球状粒子TitansphereTiO，

则可从蛋白质酶切物中选择性地浓缩精制磷酸化肽，使MS检测变得容易。

操作方法全部为离心操作，几乎没有人为误差。处理时间约40分钟，可在短时间内完成精制? 浓缩。

磷酸化肽选择的精制浓缩珠

Titansphere TiO 填料是能够选择性地精制 浓缩磷酸化肽的高性能填料。使用拟南芥细胞提取物，与市售IMAC 进行了比较，磷酸化肽的总峰面积约2.6 倍、肽鉴定数约1.8 倍，可检测到大部分磷酸化肽。

Features

Spherically shaped porous particle “Titansphere TiO” (Titanium Dioxide ; TiO₂) is a high-performing bulk material to enrich and purify phosphopeptides selectively.

• Efficient purification from HeLa Cell Lysate

The graph below shows the superior performance of Titansphere TiO using the HeLa Cell Lysate consisting mainly of non-phosphorylated peptides. Titansphere TiO shows exceptional selectivity – almost 90% of the bound peptides were phosphopeptides, and excellent capacity for total phosphopeptide binding. Whereas a competitive TiO product binds mainly non-phosphorylated peptides and a much lower total number of discrete phosphopeptides.

Sample: HeLa Cell Lysate,  Sample volume: 50 μg,  Titansphere TiO volume: 1 mg

• Comparison of Titansphere TiO and IMAC

The graph below shows the comparison of Titansphere TiO and IMAC enrichment using Arabidopsis cell extract. Titansphere TiO provides substantially higher total capacity and a much higher number of isolated phosphopeptides.

Sample: Arabidopsis Cell Extract,  Sample volume: 100 μg,  Titansphere TiO volume: 1 mg

• Example of how to make a Titanium Column
  ? Cut the Empore Disk C8 and put it at the tip of the 200ul pipette tip to be used as a filter.
  ? Stir and suspend the Titansphere TiO atCH3CN/H2O=80/20.v/v then load it to the tip.
    (Adjust the amounf of the beads depending on the purpose)
  ? Pressure the tip by a plastic syringe.

  

  
  

  

Specifications

订货信息

Identified Numbers of Phosphopeptides

Titansphere^™ TiO Part Number

* New material for higher purification efficiency of Phosphopeptides

Empore Disk C8 Part Number