人抗酒石酸酸性磷酸酶(ACP5)ELISA试剂盒

人抗酒石酸酸性磷酸酶(ACP5)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人抗酒石酸酸性磷酸酶(ACP5)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人抗酒石酸酸性磷酸酶(ACP5)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人抗酒石酸酸性磷酸酶(ACP5)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人抗酒石酸酸性磷酸酶(ACP5)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H1103c 人叉头框蛋白P1(FOXP1)ELISA试剂盒 Human FOXP1 (Forkhead Box Protein P1) ELISA Kit 

XY-EL-H1104c 人叉头框蛋白P3(FOXP3)ELISA试剂盒 Human FOXP3 (Forkhead Box Protein P3) ELISA Kit 

XY-EL-H1105c 人长效甲状腺刺激素(LATS)ELISA试剂盒 Human LATS (Long Acting Thyroid Stimulator) ELISA Kit

XY-EL-H1106c 人肝配蛋白A4 (EFNA4)ELISA试剂盒 Human EFNA4 (Ephrin A4) ELISA Kit 

XY-EL-H1108c 人肠三叶因子3(TFF3)ELISA试剂盒 Human TFF3 (Trefoil Factor 3, Intestinal ) ELISA Kit

XY-EL-H1110c 人转胶蛋白(TAGLN)ELISA试剂盒 Human TAGLN (Transgelin) ELISA Kit

XY-EL-H1111c 人端粒酶相关蛋白1(TEP1)ELISA试剂盒 Human TEP1 (Telomerase Associated Protein 1) ELISA Kit

XY-EL-H1112c 人碱性唾液脯氨酸丰富蛋白2(PRB2)ELISA试剂盒 Human PRB2 (Basic Salivary Proline Rich Protein 2) ELISA Kit

XY-EL-H1113c 人超氧化物歧化酶1(SOD1)ELISA试剂盒 Human SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit

XY-EL-H1114c 人成骨生长肽/成骨多肽(OGP)ELISA试剂盒 Human OGP (Osteogenic Growth Peptide) ELISA Kit

XY-EL-H1115c 人成熟促进因子(MPF)ELISA试剂盒 Human MPF (Maturation Promoting Factor) ELISA Kit

XY-EL-H1116c 人成纤维细胞生长因子23(FGF23)ELISA试剂盒 Human FGF23 (Fibroblast Growth Factor 23) ELISA Kit

XY-EL-H1117c 人成纤维细胞生长因子9(FGF9)ELISA试剂盒 Human FGF9 (Fibroblast Growth Factor 9) ELISA Kit 

XY-EL-H1118c 人成纤维细胞生长因子结合蛋白1(FGFBP1)ELISA试剂盒 Human FGFBP1 (Fibroblast Growth Factor Binding Protein 1) ELISA Kit 

XY-EL-H1119c 人成纤维细胞生长因子结合蛋白2(FGFBP2)ELISA试剂盒 Human FGFBP2 (Fibroblast Growth Factor Binding Protein 2) ELISA Kit 

XY-EL-H1120c 人成纤维细胞生长因子受体底物2(FRS2)ELISA试剂盒 Human FRS2 (Fibroblast Growth Factor Receptor Substrate 2) ELISA Kit

XY-EL-H1121c 人成纤维细胞生长因子受体底物3(FRS3)ELISA试剂盒 Human FRS3 (Fibroblast Growth Factor Receptor Substrate 3) ELISA Kit 

XY-EL-H1122c 人迟现抗原(VLA)ELISA试剂盒 Human VLA (Very Late Appearing Antigen) ELISA Kit

XY-EL-H1123c 人穿孔蛋白1/成孔蛋白(PRF1/PFP)ELISA试剂盒 Human PRF1 (Perforin 1/Pore-Forming Protein) ELISA Kit

XY-EL-H1124c 人卵泡刺激素受体(FSHR)ELISA试剂盒 Human FSHR (Follicle Stimulating Hormone Receptor) ELISA Kit 

XY-EL-H1125c 人垂草扁桃酸(VMA)ELISA试剂盒 Human VMA (Vanillylmandelic Acid) ELISA Kit

XY-EL-H1126c 人角质转谷氨酰胺酶(TGM1)ELISA试剂盒 Human TGM1 (Transglutaminase 1, Keratinocyte) ELISA Kit

XY-EL-H1127c 人垂体腺苷酸环化酶激活肽受体 (PACAPR)ELISA试剂盒 Human PACAPR (Pituitary Adenylate Cyclase Activating Polypeptide Receptor 1) ELISA Kit 

XY-EL-H1129c 人肝配蛋白A受体10 (EPHA10)ELISA试剂盒 Human EPHA10 (Ephrin Type A Receptor 10) ELISA Kit 

XY-EL-H1132c 人雌激素受体β(ERβ)ELISA试剂盒 Human ERβ (Estrogen Receptor Beta) ELISA Kit

XY-EL-H1133c 人雌激素受体α(ERα)ELISA试剂盒 Human ERα (Estrogen Receptor Alpha) ELISA Kit 

XY-EL-H1134c 人雌激素诱导蛋白PS2 (EIPS2)ELISA试剂盒 Human EIPS2 (Estrogen Induced Protein pS2) ELISA Kit

XY-EL-H1136c 人肝配蛋白A5 (EFNA5)ELISA试剂盒 Human EFNA5 (Ephrin A5) ELISA Kit 

XY-EL-H1137c 人过氧化还原酶2(PRDX2)ELISA试剂盒 Human PRDX2 (Peroxiredoxin 2) ELISA Kit

XY-EL-H1138c 人丝裂原激活蛋白激酶激活蛋白激酶3 (MAPKAPK3)ELISA试剂盒 Human MAPKAPK3 (Mitogen Activated Protein Kinase Activated Protein Kinase 3) ELISA Kit

XY-EL-H1139c 人促甲状腺激素抗体(TSA)ELISA试剂盒 Human TSA (Thyroid Stimulating Antibody) ELISA Kit

XY-EL-H1140c 人促甲状腺激素胚胎因子(TEF)ELISA试剂盒 Human TEF (Thyrotroph Embryonic Factor) ELISA Kit 

XY-EL-H1141c 人促甲状腺素(TSH)ELISA试剂盒 Human TSH (Thyroid Stimulating Hormone) ELISA Kit 

XY-EL-H1142c 人促甲状腺素释放激素(TRH)ELISA试剂盒 Human TRH (Thyrotropin Releasing Hormone) ELISA Kit

XY-EL-H1143c 人促卵泡素(FSH)ELISA试剂盒 Human FSH (Follicle-Stimulating Hormone) ELISA Kit 

XY-EL-H1144c 人促肾上皮质激素释放激素(CRH)ELISA试剂盒 Human CRH (Corticotropin Releasing Hormone) ELISA Kit

XY-EL-H1146c 人促生长激素释放激素(GHRH)ELISA试剂盒 Human GHRH (Growth Hormone Releasing Hormone) ELISA Kit