人白介素11(IL-11)检测试剂盒实验操作常见问题分析
异常 | 结果描述 | 原因分析 | 对策 |
白板 | 显色步骤结束后,酶标板所有孔均无颜色。阳性对照不显色。 | 试剂已过效期,或不同试剂盒组分混用 | 检查试剂的组分及批号,确认未过期以及所有组分均属对应的试剂盒。不同试剂盒或不同批号的试剂不能混用。 |
错加、漏加试剂底物、显色剂 A或B | 严格按说明书的步骤操作,加完试剂后可观察一下液面高度是否一致,以减少漏加现象。 |
洗板及加样过程中,酶标受污染失活失去催化显色剂显色的能力 | 确认盛酶标的容器不含酶抑制剂如叠氮钠等,确认配制洗液的容器已洗干净。 |
终止液误作洗涤液稀释或当底物缓冲液配制 | 每次配制时都人白介素11(IL-11)检测试剂盒应看清标签标明物质 |
蒸馏水有问题 | 确认配制洗液的纯化水达到要求且未污染,与好蒸馏水比较。 |
标本及采集、贮运因素:严重溶血 ,以 HRP 为标记的ELISA 测定中,残留在孔内的血红蛋白具有过氧化物酶样活性,催化底物显色造成假阳性; 混有红细胞的血清 易沉淀或附着在聚乙烯孔内不易洗净;如有 细菌污染 ,菌体中可能含有内源性HRP,也会产生假阳性反应; 标本凝固不全 ,有时为了争取时间快速检测,常在血液还未开始凝固时即强行离心分离血清,使血清中仍残留部分纤维蛋白原,在ELISA测定过程中可以形成肉眼可见的纤维蛋白块,易造成假阳性结果; 采血试管洗涤不彻底 、反复使用易交叉污染; 塑料试管能吸附抗原物质 ,样本久置在塑料管内会使样本内抗原含量下降造成假阴性。
人白介素11(IL-11)检测试剂盒其它相关试剂盒:
XYA527Mu小鼠氧化低密度脂蛋白(OxLDL)ELISA试剂盒ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)
XYB908Mu小鼠聚集蛋白聚糖(AGC)ELISA试剂盒ELISA Kit for Aggrecan (AGC)
XYA411Mu小鼠钙非依赖性磷脂酶A2(iPLA2)ELISA试剂盒ELISA Kit for Phospholipase A2, Calcium Independent (iPLA2)
XYA842Mu小鼠脂蛋白a(Lpa)ELISA试剂盒ELISA Kit for Lipoprotein, a (Lpa)
XYA204Mu小鼠生长停滞特异性蛋白6(GAS6)ELISA试剂盒ELISA Kit for Growth Arrest Specific Protein 6 (GAS6)
XYB706Mu小鼠脂质运载蛋白1(L人白介素11(IL-11)检测试剂盒CN1)ELISA试剂盒ELISA Kit for Lipocalin 1 (LCN1)
XYA622Mu小鼠谷胱甘肽S转移酶θ1(GSTθ1)ELISA试剂盒ELISA Kit for Glutathione S Transferase Theta 1 (GSTt1)
XYB983Mu小鼠微管关联蛋白τ(MAPτ)ELISA试剂盒ELISA Kit for Microtubule Associated Protein Tau (MAPt)
XYB995Mu小鼠血幼素(HJV)ELISA试剂盒ELISA Kit for Hemojuvelin (HJV)
XYA681Mu小鼠乙醇脱氢酶1(ADH1)ELISA试剂盒ELISA Kit for Alcohol Dehydrogenase 1 (ADH1)
XYC024Mu小鼠线粒体乙醛脱氢酶(ALDM)ELISA试剂盒ELISA Kit for Aldehyde Dehydrogenase, Mitochondrial (ALDM)
XYE866Mu小鼠乙醇脱氢酶7(ADH7)ELISA试剂盒ELISA Kit for Alcohol Dehydrogenase 7 (ADH7)
XYB329Mu小鼠微管关联蛋白2(MAP2)ELISA试剂盒ELISA Kit for Microtubule Associated Protein 2 (MAP2)
XYA645Mu小鼠晚期糖基化终末产物受体(AGER)ELISA试剂盒ELISA Kit for Advanced Glycosylation End Product Specific Receptor (AGER)
XYA694Mu小鼠含血管性血友病因子A域蛋白3A(vWA3A)ELISA试剂盒ELISA Kit for Von Willebrand Factor A Domain Containing Protein 3A (vWA3A)
XYA139Mu小鼠肿瘤坏死因子相关凋亡诱导配体(TRAIL)ELISA试剂盒ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
XYA919Mu小鼠二氢硫辛酸脱氢酶(DLD)ELISA试剂盒ELISA Kit for Dihydrolipoyl Dehydrogenase (DLD)
XYB539Mu小鼠血清淀粉样P物质(SAP)ELISA试剂盒ELISA Kit for Serum Amyloid P Component (SAP)
XYB265Mu小鼠胰多肽(PP)ELISA试剂盒ELISA Kit for Pancreatic Polypeptide (PP)
XYB311Mu小鼠阿黑皮素原(POMC)ELISA试剂盒ELISA Kit for Proopiomelanocortin (POMC)
XYB686Mu小鼠B-细胞激活因子(BAFF)ELISA试剂盒ELISA Kit for B-Cell Activating Factor (BAFF)
XYA442Mu小鼠阻抑素(PHB)ELISA试剂盒ELISA Kit for Prohibitin (PHB)
XYB685Mu小鼠整合素αM(ITGαM)ELISA试剂盒ELISA Kit for Integrin Alpha M (ITGaM)
XYB966Mu小鼠多配体蛋白聚糖1(SDC1)ELISA试剂盒ELISA Kit for Syndecan 1 (SDC1)
XYA977Mu小鼠盘状结构域受体家族成员1(DDR1)ELISA试剂盒ELISA Kit for Discoidin Domain Receptor Family, Member 1 (DDR1)
XYB317Mu小鼠穿孔素1(PRF1)ELISA试剂盒ELISA Kit for Perforin 1 (PRF1)
XYB017Mu小鼠α2-巨球蛋白(α2M)ELISA试剂盒ELISA Kit for Alpha-2-Macroglobulin (a2M)
XYB063Mu小鼠泛素结合酶E2A(UBE2A)ELISA试剂盒ELISA Kit for Ubiquitin Conjugating Enzyme E2A (UBE2A)
XYG208Mu小鼠叉头框蛋白A2(FOXA2)ELISA试剂盒ELISA Kit for Forkhead Box Protein A2 (FOXA2)