人克拉拉细胞蛋白(CC16)ELISA试剂盒

Human CC16 FOR RESEARCH USE ONLY Assay range：3ng/ml - 80ng/ml 96 determinations Purpose This kit allows for the determination of CC16 concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human CC16 level in the sample，use Purified Human CC16 antibody to coat microtiter plate wells, make solid-phase antibody, then add CC16 to wells, Combined CC16 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human CC16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.