大鼠干细胞因子(SCF)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠干细胞因子(SCF)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠干细胞因子(SCF)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠干细胞因子(SCF)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠干细胞因子(SCF)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK0207c 猴信号传导转录激活因子1(STAT1)检测试剂盒 Monkey STAT1 (Signal Transducer and Activator of Transcription 1) ELISA Kit
HZ-EL-MK0208c 猴胆固醇(CH)检测试剂盒 Monkey CH (Cholesterol) ELISA Kit
HZ-EL-MK0209c 猴转录激活因子-3(ATF-3)检测试剂盒 Monkey ATF-3 (Activating Transcription Factor 3) ELISA Kit
HZ-EL-MK0210c 猴β-促脂素(β-LPH)检测试剂盒 Monkey β-LPH (β-Lipotropic Hormone) ELISA Kit
HZ-EL-MK0211c 猴糖原合成酶激酶3β(GSK3β)检测试剂盒 Monkey GSK3β (Glycogen Synthase Kinase 3 Beta) ELISA Kit
HZ-EL-MK0212c 猴SPARC样蛋白1(SPARCL1)检测试剂盒 Monkey SPARCL1 (SPARC Like Protein 1) ELISA Kit
HZ-EL-MK0214c 猴VI型胶原(COL6)检测试剂盒 Monkey COL6 (Collagen Type VI) ELISA Kit
HZ-EL-MK0215c 猴补体组分1Q受体1(C1qR1)检测试剂盒 Monkey C1qR1 (Complement Component 1q Receptor) ELISA Kit
HZ-EL-MK0216c 猴肌联蛋白抗体(TTN)检测试剂盒 Monkey TTN (Titin) ELISA Kit
HZ-EL-MK0217c 猴细胞色素P450家族成员1A2(CYP1A2)检测试剂盒 Monkey CYP1A2 (Cytochrome P450 1A2) ELISA Kit
HZ-EL-MK0219c 猴丝氨酸蛋白酶33(PRSS33)检测试剂盒 Monkey PRSS33 (Protease, Serine 33) ELISA Kit
HZ-EL-MK0220c 猴活化蛋白C抵抗素(APCR)检测试剂盒 Monkey APCR (Activated Protein C Resistance) ELISA Kit
HZ-EL-MK0221c 猴γ谷氨酰半胱氨酸合成酶(γ-ECS)检测试剂盒 Monkey γ-ECS (γ-Glutamyl Systeine Synthetase) ELISA Kit
HZ-EL-MK0222c 猴性别决定区Y框蛋白18(SOX18)检测试剂盒 Monkey SOX18 (Sex Determining Region Y Box Protein 18) ELISA Kit
HZ-EL-MK0223c 猴5羟基吲哚乙酸(5-HIAA)检测试剂盒 Monkey 5-HIAA (5-Hydroxyindolacetic Acid) ELISA Kit
HZ-EL-MK0224c 猴基质金属蛋白酶-1(MMP1)检测试剂盒 Monkey MMP-1 (Matrix Metalloproteinase 1) ELISA Kit
HZ-EL-MK0225c 猴V型胶原α2(COL5α2)检测试剂盒 Monkey COL5α2 (Collagen Type V Alpha 2) ELISA Kit
HZ-EL-MK0227c 猴胶质细胞系来源的神经营养因子(GDNF)检测试剂盒 Monkey GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
HZ-EL-MK0228c 猴基膜聚糖(LUM)检测试剂盒 Monkey LUM (Lumican) ELISA Kit
HZ-EL-MK0229c 猴细胞外信号调节激酶3(ERK3)检测试剂盒 Monkey ERK3 (Extracellular Signal Regulated Kinase 3) ELISA Kit
HZ-EL-MK0230c 猴糖化血红蛋白(HbA1c)检测试剂盒 Monkey HbA1c (Glycosylated Hemoglobin/Hemoglobin A1c) ELISA Kit
HZ-EL-MK0231c 猴紧密连接蛋白Claudin 4(CLDN4)检测试剂盒 Monkey CLDN4 (Claudin 4) ELISA Kit
HZ-EL-MK0232c 猴谷胱甘肽过氧化物酶 1(GPX1)检测试剂盒 Monkey GPX1 (Glutathione Peroxidase 1) ELISA Kit
HZ-EL-MK0233c 猴抗甲状腺过氧化物酶抗体(TPO-Ab)检测试剂盒 Monkey TPO-Ab (Anti-Thyroid-Peroxidase Antibody) ELISA Kit
HZ-EL-MK0234c 猴肾上腺皮质发育异常蛋白(ACD)检测试剂盒 Monkey ACD (Adrenocortical Dysplasia Homolog) ELISA Kit
HZ-EL-MK0235c 猴脂蛋白a(LP-a)检测试剂盒 Monkey LP-a (Lipoprotein a) ELISA Kit
HZ-EL-MK0236c 猴核糖体蛋白L10(RPL10)检测试剂盒 Monkey RPL10 (Ribosomal Protein L10) ELISA Kit