大鼠干细胞因子受体(SCFR)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠干细胞因子受体(SCFR)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠干细胞因子受体(SCFR)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠干细胞因子受体(SCFR)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠干细胞因子受体(SCFR)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK0176c 猴粘蛋白20(MUC20)检测试剂盒 Monkey MUC20 (Mucin 20) ELISA Kit
HZ-EL-MK0177c 猴心肌营养素合成酶/心磷脂合酶(CLS)检测试剂盒 Monkey CLS (Cardiolipin Synthase) ELISA Kit
HZ-EL-MK0179c 猴精氨酰tRNA合成酶(RARS)检测试剂盒 Monkey RARS (Arginyl tRNA Synthetase) ELISA Kit
HZ-EL-MK0180c 猴结缔组织生长因子(CTGF)检测试剂盒 Monkey CTGF (Connective Tissue Growth Factor) ELISA Kit
HZ-EL-MK0181c 猴白血病抑制因子(LIF)检测试剂盒 Monkey LIF (Leukemia Inhibitory Factor) ELISA Kit
HZ-EL-MK0182c 猴IV型胶原α1(COL4α1)检测试剂盒 Monkey COL4α1 (Collagen Type IV Alpha 1) ELISA Kit
HZ-EL-MK0183c 猴过氧化物酶体膜蛋白3(PMP3)检测试剂盒 Monkey PMP3 (Peroxisomal Membrane Protein 3) ELISA Kit
HZ-EL-MK0184c 猴表皮角蛋白(EK)检测试剂盒 Monkey EK (Eepidermal Keratin) ELISA Kit
HZ-EL-MK0185c 猴环腺苷二磷酸核糖水解酶(cADPRH/CD38)检测试剂盒 Monkey cADPRH/CD38 (Cyclic ADP Hydrolase) ELISA Kit
HZ-EL-MK0187c 猴神经丝蛋白(NF)检测试剂盒 Monkey NF (Neurofilament) ELISA Kit
HZ-EL-MK0188c 猴血管内皮生长因子受体2(VEGFR-2/KDR)检测试剂盒 Monkey VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
HZ-EL-MK0189c 猴抗麦胶蛋白/麦醇溶蛋白抗体(AGA)检测试剂盒 Monkey AGA (Anti-Gliadin Antibody) ELISA Kit
HZ-EL-MK0190c 猴胰岛素样生长因子2(IGF-2)检测试剂盒 Monkey IGF-2 (Insulin Like Growth Factor 2) ELISA Kit
HZ-EL-MK0191c 猴信号传导转录激活因子6B(STAT56B)检测试剂盒 Monkey STAT6B (Signal Transducer and Activator of Transcription 6B) ELISA Kit
HZ-EL-MK0192c 猴补体成分4a(C4a)检测试剂盒 Monkey C4a (Complement Component 4a) ELISA Kit
HZ-EL-MK0193c 猴肝配蛋白A3 (EFNA3)检测试剂盒 Monkey EFNA3 (Ephrin A3) ELISA Kit
HZ-EL-MK0194c 猴N-乙酰α-D-氨基葡萄糖苷酶(NAGLU)检测试剂盒 Monkey NAGLU (N-Acetyl Alpha-D-Glucosaminidase) ELISA Kit
HZ-EL-MK0195c 猴斑型POZ蛋白(SPOP)检测试剂盒 Monkey SPOP (Speckle Type POZ Protein) ELISA Kit
HZ-EL-MK0196c 猴基质金属蛋白酶12(MMP-12)检测试剂盒 Monkey MMP-12 (Matrix Metalloproteinase 12) ELISA Kit
HZ-EL-MK0197c 猴周期素依赖性激酶8(CDK8)检测试剂盒 Monkey CDK8 (Cyclin Dependent Kinase 8) ELISA Kit
HZ-EL-MK0198c 猴肝配蛋白A5 (EFNA5)检测试剂盒 Monkey EFNA5 (Ephrin A5) ELISA Kit
HZ-EL-MK0200c 猴骨骼肌快肌肌钙蛋白I(TNNI2)检测试剂盒 Monkey TNNI2 (Troponin I Type 2, Fast Skeletal) ELISA Kit
HZ-EL-MK0201c 猴胸腺嘧啶核苷磷酸化酶(TP)检测试剂盒 Monkey TP (Thymidine Phosphorylase) ELISA Kit
HZ-EL-MK0202c 猴结肠癌抗原(CCA)检测试剂盒 Monkey CCA (Colon Cancer Antigen) ELISA Kit
HZ-EL-MK0203c 猴核孔蛋白37kda(NUP37)检测试剂盒 Monkey NUP37 (Nucleoporin 37kDa) ELISA Kit
HZ-EL-MK0205c 猴促性腺激素释放激素(GnRH)检测试剂盒 Monkey GnRH (Gonadotropin Releasing Hormone) ELISA Kit
HZ-EL-MK0206c 猴蛋白激酶C(PKC)检测试剂盒 Monkey PKC (Protein Kinase C) ELISA Kit