大鼠多肽YY(PYY)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠多肽YY(PYY)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠多肽YY(PYY)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠多肽YY(PYY)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠多肽YY(PYY)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-RB1106c 兔鸟氨酸脱羧酶(ODC)检测试剂盒 Rabbit ODC (Ornithine Decarboxylase) ELISA Kit
HZ-EL-RB1107c 兔胆碱磷酸甘油酯(PC/CPG)检测试剂盒 Rabbit PC/CPG (Choline Phosphoglyceride) ELISA Kit
HZ-EL-RB1108c 兔Rho家族GTP酶1(RND1)检测试剂盒 Rabbit RND1 (Rho Family GTPase 1) ELISA Kit
HZ-EL-RB1109c 兔胰岛素样生长因子1受体(IGF1R)检测试剂盒 Rabbit IGF1R (Insulin Like Growth Factor 1 Receptor) ELISA Kit
HZ-EL-RB1110c 兔转铁蛋白受体(TFR/CD71)检测试剂盒 Rabbit TFR/CD71 (Transferrin Receptor) ELISA Kit
HZ-EL-RB1111c 兔肝配蛋白A4 (EFNA4)检测试剂盒 Rabbit EFNA4 (Ephrin A4) ELISA Kit
HZ-EL-RB1112c 兔三叶因子2(TFF2)检测试剂盒 Rabbit TFF2 (Trefoil Factor 2) ELISA Kit
HZ-EL-RB1114c 兔单核细胞趋化蛋白1(MCP-1)检测试剂盒 Rabbit MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
HZ-EL-RB1115c 兔胰岛素样生长因子结合蛋白6(IGFBP-6)检测试剂盒 Rabbit IGFBP-6 (Insulin Like Growth Factor Binding Protein 6) ELISA Kit
HZ-EL-RB1117c 兔抗甲状腺球蛋白抗体(ATGA/TGAB)检测试剂盒 Rabbit ATGA/TGAB (Anti-Thyroid-Globulin Antibody) ELISA Kit
HZ-EL-RB1118c 兔补体片断5b(C5b)检测试剂盒 Rabbit C5b (Complement Fragment 5b) ELISA Kit
HZ-EL-RB1119c 兔衰变加速因子(DAF)检测试剂盒 Rabbit DAF (Decay Accelerating Factor) ELISA Kit
HZ-EL-RB1120c 兔BCL2修饰因子(BMF)检测试剂盒 Rabbit BMF (Bcl-2 Modifying Factor) ELISA Kit
HZ-EL-RB1121c 兔Bcl2关联永生基因3(BAG3)检测试剂盒 Rabbit BAG3 (Bcl2 Associated Athanogene 3) ELISA Kit
HZ-EL-RB1122c 兔叉头框蛋白P3(FOXP3)检测试剂盒 Rabbit FOXP3 (Forkhead Box Protein P3) ELISA Kit
HZ-EL-RB1123c 兔嗜环蛋白/亲环素A(CYPA)检测试剂盒 Rabbit CYPA (Cyclophilin A) ELISA Kit
HZ-EL-RB1125c 兔天青杀素1(AZU1)检测试剂盒 Rabbit AZU1 (Azurocidin 1) ELISA Kit
HZ-EL-RB1126c 兔抑制蛋白β1(ARRβ1)检测试剂盒 Rabbit ARRβ1 (Arrestin Beta 1) ELISA Kit
HZ-EL-RB1127c 兔早老素2(PS2)检测试剂盒 Rabbit PS2 (Presenilin 2) ELISA Kit
HZ-EL-RB1128c 兔蛋白磷酸酶1调控亚基1A(PPP1R1A)检测试剂盒 Rabbit PPP1R1A (Protein Phosphatase 1 Regulatory Subunit A) ELISA Kit
HZ-EL-RB1129c 兔胸肾表达趋化因子(BRAK/CXCL14)检测试剂盒 Rabbit BRAK/CXCL14 (Breast and Kidney Expressed Chemokine) ELISA Kit
HZ-EL-RB1130c 兔间隙连接蛋白β1(GJβ1)检测试剂盒 Rabbit GJβ1 (Gap Junction Protein Beta 1) ELISA Kit
HZ-EL-RB1131c 兔着丝粒蛋白E(CENPE)检测试剂盒 Rabbit CENPE (Centromere Protein E) ELISA Kit
HZ-EL-RB1132c 兔凝血因子Ⅹ(F10)检测试剂盒 Rabbit F10 (Coagulation Factor X) ELISA Kit
HZ-EL-RB1133c 兔胆囊收缩素/肠促胰酶肽A受体(CCKAR)检测试剂盒 Rabbit CCKAR (Cholecystokinin A Receptor) ELISA Kit
HZ-EL-RB1134c 兔蛋白激酶C-η(PKCη)检测试剂盒 Rabbit PKCη (Protein Kinase C Eta ) ELISA Kit