大鼠戊糖素(PTD)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠戊糖素(PTD)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠戊糖素(PTD)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠戊糖素(PTD)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠戊糖素(PTD)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-RB1049c 兔血栓调节蛋白(TM)检测试剂盒 Rabbit TM (Thrombomodulin ) ELISA Kit
HZ-EL-RB1050c 兔抑瘤素M受体(OSMR)检测试剂盒 Rabbit OSMR (Oncostatin M Receptor) ELISA Kit
HZ-EL-RB1051c 兔细胞角蛋白17(CK17/KRT17)检测试剂盒 Rabbit CK-17/KRT17 (Cytokeratin 17) ELISA Kit
HZ-EL-RB1053c 兔乳酸脱氢酶C(LDHC)检测试剂盒 Rabbit LDHC (Lactate Dehydrogenase C) ELISA Kit
HZ-EL-RB1054c 兔乙酰辅酶A乙酰转移酶2(ACAT2)检测试剂盒 Rabbit ACAT2 (Acetyl Coenzyme A Acetyltransferase 2) ELISA Kit
HZ-EL-RB1055c 兔促生长激素释放激素(GHRH)检测试剂盒 Rabbit GHRH (Growth Hormone Releasing Hormone) ELISA Kit
HZ-EL-RB1056c 兔金属硫蛋白1E (MT1E)检测试剂盒 Rabbit MT1E (Metallothionein 1E) ELISA Kit
HZ-EL-RB1058c 兔乙醇脱氢酶1(ADH1)检测试剂盒 Rabbit ADH1 (Alcohol Dehydrogenase 1) ELISA Kit
HZ-EL-RB1059c 兔碳酸酐酶II(CA2)检测试剂盒 Rabbit CA2 (Carbonic Anhydrase II) ELISA Kit
HZ-EL-RB1060c 兔甲状腺过氧化物酶(TPO)检测试剂盒 Rabbit TPO (Thyroid Peroxidase) ELISA Kit
HZ-EL-RB1061c 兔基质金属蛋白酶原10 (Pro-MMP-10)检测试剂盒 Rabbit Pro-MMP-10 (Pro-Matrix Metalloproteinase 10) ELISA Kit
HZ-EL-RB1062c 兔二肽基肽酶VI (DPP6)检测试剂盒 Rabbit DPP6 (Dipeptidyl Peptidase VI) ELISA Kit
HZ-EL-RB1063c 兔生长因子受体结合蛋白10(Grb10)检测试剂盒 Rabbit Grb10 (Growth Factor Receptor Bound Protein 10) ELISA Kit
HZ-EL-RB1064c 兔不均一核糖核蛋白U (HNRPU)检测试剂盒 Rabbit HNRPU (Heterogeneous Nuclear Ribonucleoprotein U) ELISA Kit
HZ-EL-RB1065c 兔骨成型蛋白受体II(BMPR2)检测试剂盒 Rabbit BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit
HZ-EL-RB1066c 兔原钙黏素β2(PCDHβ2)检测试剂盒 Rabbit PCDHβ2 (Protocadherin Beta 2) ELISA Kit
HZ-EL-RB1067c 兔载脂蛋白C2(ApoC2)检测试剂盒 Rabbit ApoC2 (Apolipoprotein C2) ELISA Kit
HZ-EL-RB1068c 兔神经胶质纤维酸性蛋白(GFAP)检测试剂盒 Rabbit GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
HZ-EL-RB1069c 兔粘蛋白4(MUC4)检测试剂盒 Rabbit MUC4 (Mucin 4) ELISA Kit
HZ-EL-RB1070c 兔铁调素(Hepc)检测试剂盒 Rabbit Hepc (Hepcidin) ELISA Kit
HZ-EL-RB1071c 兔凝血因子XIII B多肽(F13B)检测试剂盒 Rabbit F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit
HZ-EL-RB1072c 兔肌营养不良蛋白关联糖蛋白1(DAG1)检测试剂盒 Rabbit DAG1 (Dystrophin Associated Glycoprotein 1) ELISA Kit
HZ-EL-RB1073c 兔甘氨酰tRNA合成酶(GARS)检测试剂盒 Rabbit GARS (Glycyl tRNA Synthetase) ELISA Kit
HZ-EL-RB1074c 兔视黄醇结合蛋白4(RBP4)检测试剂盒 Rabbit RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit
HZ-EL-RB1075c 兔大脑脂肪酸结合蛋白7(FABP7)检测试剂盒 Rabbit FABP7 (Fatty Acid Binding Protein 7, Brain) ELISA Kit
HZ-EL-RB1076c 兔窖蛋白(Cav-1)检测试剂盒 Rabbit Cav-1 (Caveolin-1) ELISA Kit