大鼠甲状旁腺激素(PTH)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠甲状旁腺激素(PTH)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠甲状旁腺激素(PTH)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠甲状旁腺激素(PTH)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠甲状旁腺激素(PTH)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-RB0922c 兔Rap鸟嘌呤交换因子1(RAPGEF1)检测试剂盒 Rabbit RAPGEF1 (Rap Guanine Nucleotide Exchange Factor 1) ELISA Kit
HZ-EL-RB0923c 兔多聚血清蛋白(PHSA)检测试剂盒 Rabbit PHSA (Poly Serium Antigen) ELISA Kit
HZ-EL-RB0924c 兔血小板膜糖蛋白VI(GP6)检测试剂盒 Rabbit GP6 (Glycoprotein VI, Platelet) ELISA Kit
HZ-EL-RB0925c 兔肠三叶肽因子3(TFF3)检测试剂盒 Rabbit TFF3 (Trefoil Factor 3, Intestinal ) ELISA Kit
HZ-EL-RB0926c 兔X型胶原(COL10)检测试剂盒 Rabbit COL10 (Collagen Type X) ELISA Kit
HZ-EL-RB0928c 兔纤溶酶原激活物抑制因子2(PAI2)检测试剂盒 Rabbit PAI2 (Plasminogen Activator Inhibitor 2 )ELISA Kit
HZ-EL-RB0929c 兔STAT蛋白抑制因子1(PIAS1)检测试剂盒 Rabbit PIAS1 (Protein Inhibitor of Activated STAT 1) ELISA Kit
HZ-EL-RB0931c 兔早期生长应答蛋白4(EGR4)检测试剂盒 Rabbit EGR4 (Early Growth Response Protein 4) ELISA Kit
HZ-EL-RB0932c 兔肌球蛋白重链1(MYH1)检测试剂盒 Rabbit MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult) ELISA Kit
HZ-EL-RB0933c 兔内皮素1(ET-1)检测试剂盒 Rabbit ET-1 (Endothelin 1) ELISA Kit
HZ-EL-RB0934c 兔前列腺素内过氧化物合酶1(PTGS1)检测试剂盒 Rabbit PTGS1 (Prostaglandin Endoperoxide Synthase 1) ELISA Kit
HZ-EL-RB0935c 兔孕激素相关子宫内膜蛋白(PAEP)检测试剂盒 Rabbit PAEP (Progestagen-Associated Endometrial Protein) ELISA Kit
HZ-EL-RB0936c 兔颗粒酶K(GZMK)检测试剂盒 Rabbit GZMK (Granzyme K) ELISA Kit
HZ-EL-RB0937c 兔剪切修复交叉互补基因1(ERCC1)检测试剂盒 Rabbit ERCC1 (Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1) ELISA Kit
HZ-EL-RB0939c 兔趋化因子C-C-基元配体1(CCL1)检测试剂盒 Rabbit CCL1 (Chemokine C-C-Motif Ligand 1) ELISA Kit
HZ-EL-RB0941c 兔血小板衍生生长因子AB(PDGF-AB)检测试剂盒 Rabbit PDGF-AB (Platelet Derived Growth Factor AB) ELISA Kit
HZ-EL-RB0942c 兔纤维胶凝蛋白1(FCN1)检测试剂盒 Rabbit FCN1 (Ficolin 1) ELISA Kit
HZ-EL-RB0943c 兔优球蛋白(EL)检测试剂盒 Rabbit EL (EuglobulinLysis) ELISA Kit
HZ-EL-RB0944c 兔早期生长应答蛋白2(EGR2)检测试剂盒 Rabbit EGR2 (Early Growth Response Protein 2) ELISA Kit
HZ-EL-RB0945c 兔肌钙蛋白I(Tn-I)检测试剂盒 Rabbit Tn-I (Troponin I) ELISA Kit
HZ-EL-RB0946c 兔周期素依赖性激酶2(CDK2)检测试剂盒 Rabbit CDK2 (Cyclin Dependent Kinase 2) ELISA Kit
HZ-EL-RB0947c 兔富含半胱氨酸型酸性蛋白(SPARC)检测试剂盒 Rabbit SPARC (Secreted Protein Acidic And Rich In Cysteine) ELISA Kit
HZ-EL-RB0948c 兔丙氨酸氨肽酶(AAP)检测试剂盒 Rabbit AAP (Alanine Aminopeptidase) ELISA Kit
HZ-EL-RB0949c 兔核孔蛋白85kda(NUP85)检测试剂盒 Rabbit NUP85 (Nucleoporin 85kDa) ELISA Kit
HZ-EL-RB0950c 兔细胞角蛋白19(CK-19/KRT19)检测试剂盒 Rabbit CK-19/KRT19 (Cytokeratin 19) ELISA Kit
HZ-EL-RB0951c 兔XI型胶原α1(COL11α1)检测试剂盒 Rabbit COL11α1 (Collagen Type XI Alpha 1) ELISA Kit
HZ-EL-RB0952c 兔抗凝血酶(AT)检测试剂盒 Rabbit AT (AntiThrombin) ELISA Kit