大鼠单核细胞趋化蛋白1(MCP-1)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠单核细胞趋化蛋白1(MCP-1)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠单核细胞趋化蛋白1(MCP-1)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠单核细胞趋化蛋白1(MCP-1)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠单核细胞趋化蛋白1(MCP-1)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-RB0716c 兔血管内皮细胞生长因子受体1(VEGFR1/Flt1)检测试剂盒 Rabbit VEGFR1/Flt1 (Vascuoar Endothelial Growth Factor Receptor 1) ELISA Kit
HZ-EL-RB0717c 兔基质细胞衍生因子2(SDF-2)检测试剂盒 Rabbit SDF-2 (Stromal Cell Derived Factor 2) ELISA Kit
HZ-EL-RB0718c 兔血管生成素样蛋白2(ANGPTL2)检测试剂盒 Rabbit ANGPTL2 (Angiopoietin Like Protein 2) ELISA Kit
HZ-EL-RB0719c 兔过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARγC1α)检测试剂盒 Rabbit PPARγC1α (Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha) ELISA Kit
HZ-EL-RB0720c 兔全细胞色素C合酶(HCCS)检测试剂盒 Rabbit HCCS (Holocytochrome C Synthase) ELISA Kit
HZ-EL-RB0721c 兔D-多巴色素变位酶(DDT)检测试剂盒 Rabbit DDT (D-Dopachrome Tautomerase) ELISA Kit
HZ-EL-RB0722c 兔白介素23(IL-23)检测试剂盒 Rabbit IL-23 (Interleukin 23) ELISA Kit
HZ-EL-RB0723c 兔维生素D受体(VDR)检测试剂盒 Rabbit VDR (Vitamin D Receptor) ELISA Kit
HZ-EL-RB0724c 兔血管内皮细胞生长因子D(VEGF-D)检测试剂盒 Rabbit VEGF-D (Vascular Endothelial Growth Factor D) ELISA Kit
HZ-EL-RB0725c 兔磷脂酶A1(PLA1)检测试剂盒 Rabbit PLA1 (Phospholipase A1) ELISA Kit
HZ-EL-RB0726c 兔鸟苷酸交换因子(GEF)检测试剂盒 Rabbit GEF (Guanylate Exchange Factor) ELISA Kit
HZ-EL-RB0728c 兔脑多巴胺神经营养因子(CDNF)检测试剂盒 Rabbit CDNF (Cerebral Dopamine Neurotrophic Factor) ELISA Kit
HZ-EL-RB0729c 兔神经型一氧化氮合酶(NOS1/nNOS)检测试剂盒 Rabbit NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit
HZ-EL-RB0730c 兔Preptin 蛋白(Preptin)检测试剂盒 Rabbit Preptin ELISA Kit
HZ-EL-RB0732c 兔轻肽铁蛋白(FTL)检测试剂盒 Rabbit FTL (Ferritin, Light Polypeptide) ELISA Kit
HZ-EL-RB0733c 兔BTG3关联核蛋白(BANP)检测试剂盒 Rabbit BANP (BTG3 Associated Nuclear Protein) ELISA Kit
HZ-EL-RB0734c 兔核糖核酸酶III(RNASEN)检测试剂盒 Rabbit RNASEN (Ribonuclease III, Nuclear) ELISA Kit
HZ-EL-RB0735c 兔丝氨酸/苏氨酸激酶39(STK39)检测试剂盒 Rabbit STK39 (Serine/Threonine Kinase 39) ELISA Kit
HZ-EL-RB0736c 兔半乳凝集素8(GAL8)检测试剂盒 Rabbit GAL8 (Galectin 8) ELISA Kit
HZ-EL-RB0737c 兔封闭蛋白14(CLDN14)检测试剂盒 Rabbit CLDN14 (Claudin 14) ELISA Kit
HZ-EL-RB0738c 兔维生素D结合蛋白(DBP)检测试剂盒 Rabbit DBP (Vitamin D Binding Protein) ELISA Kit
HZ-EL-RB0739c 兔肿瘤蛋白p63(TP63)检测试剂盒 Rabbit TP63 (Tumor Protein p63) ELISA Kit
HZ-EL-RB0740c 兔血小板膜糖蛋白II b/III a(GPIIb/IIIa)检测试剂盒 Rabbit GPIIb/IIIa (Platelet Membrane Glycoprotein IIb/IIIa) ELISA Kit
HZ-EL-RB0741c 兔Podocin蛋白(PDCN)检测试剂盒 Rabbit PDCN (Podocin) ELISA Kit
HZ-EL-RB0742c 兔血管生成素样蛋白3(ANGPTL3)检测试剂盒 Rabbit ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit
HZ-EL-RB0743c 兔精子蛋白17(Sp17)检测试剂盒 Rabbit Sp17 (Sperm Protein 17) ELISA Kit
HZ-EL-RB0744c 兔细胞因子信号转导抑制因子3(SOCS3)检测试剂盒 Rabbit SOCS3 (Suppressors of Cytokine Signaling 3) ELISA Kit
HZ-EL-RB0745c 兔热休克蛋白90(HSP-90)检测试剂盒 Rabbit HSP-90 (Heat Shock Protein 90) ELISA Kit