大鼠连接蛋白43(CX43)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠连接蛋白43(CX43)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠连接蛋白43(CX43)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠连接蛋白43(CX43)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠连接蛋白43(CX43)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-H1253c 人泛素蛋白D(UBD)检测试剂盒 Human UBD (Ubiquitin D) ELISA Kit
HZ-EL-H1254c 人泛素分解酶(DUB)检测试剂盒 Human DUB (Deubiquitinating Enzyme) ELISA Kit
HZ-EL-H1255c 人泛素激活酶样蛋白(UBE1L)检测试剂盒 Human UBE1L (Ubiquitin Activating Enzyme E1 Like Protein) ELISA Kit
HZ-EL-H1256c 人泛素结合酶E2C(UBE2C)检测试剂盒 Human UBE2C (Ubiquitin Conjugating Enzyme E2C) ELISA Kit
HZ-EL-H1257c 人泛素连接酶E3A (UBE3A)检测试剂盒 Human UBE3A (Ubiquitin Protein Ligase E3A) ELISA Kit
HZ-EL-H1260c 人非神经元性烯醇化酶(NNE)检测试剂盒 Human NNE (Non-Neuronal Enolase) ELISA Kit
HZ-EL-H1261c 人多配体聚糖3(SDC3)检测试剂盒 Human SDC3 (Syndecan 3) ELISA Kit
HZ-EL-H1262c 人类胰蛋白酶(TPS)检测试剂盒 Human TPS (Tryptase) ELISA Kit
HZ-EL-H1263c 人衰老关键蛋白5(FBLN5)检测试剂盒 Human FBLN5 (Fibulin 5) ELISA Kit
HZ-EL-H1264c 人肺癌标志物DR-70(DR-70TM)检测试剂盒 Human DR-70TM (Tumor Marker DR-70 For Lung Cancer) ELISA Kit
HZ-EL-H1265c 人胰腺再生蛋白1β(REG1β)检测试剂盒 Human REG1β (Regenerating Islet Derived Protein 1 Beta) ELISA Kit
HZ-EL-H1266c 人肺表面活性物质相关蛋白A(SPA)检测试剂盒 Human SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit
HZ-EL-H1267c 人肺表面活性物质相关蛋白B(SPB)检测试剂盒 Human SPB (Pulmonary Surfactant Associated Protein B) ELISA Kit
HZ-EL-H1268c 人肺表面活性物质相关蛋白C(SPC)检测试剂盒 Human SPC (Pulmonary Surfactant Associated Protein C) ELISA Kit
HZ-EL-H1269c 人肺表面活性物质相关蛋白D(SPD)检测试剂盒 Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
HZ-EL-H1270c 人肺部活化调节趋化因子(PARC/CCL18)检测试剂盒 Human PARC/CCL18 (Pulmonary Activation Regulated Chemokine) ELISA Kit
HZ-EL-H1271c 人肺耐药蛋白(LRP)检测试剂盒 Human LRP (Lung Resistance-Related Protein) ELISA Kit
HZ-EL-H1272c 人丝氨酸蛋白酶8(PRSS8)检测试剂盒 Human PRSS8 (Protease, Serine 8) ELISA Kit
HZ-EL-H1273c 人SPARC样蛋白1(SPARCL1)检测试剂盒 Human SPARCL1 (SPARC Like Protein 1) ELISA Kit
HZ-EL-H1274c 人信号素3A(SEMA3A)检测试剂盒 Human SEMA3A (Semaphorin 3A) ELISA Kit
HZ-EL-H1275c 人分泌型免疫球蛋白A(sIgA)检测试剂盒 Human sIgA (Secretory Immunoglobulin A) ELISA Kit
HZ-EL-H1276c 人分泌性白细胞蛋白酶抑制因子(SLPI)检测试剂盒 Human SLPI (Secretory Leukocyte Protease Inhibitor) ELISA Kit
HZ-EL-H1277c 人低氧诱导因子1α (HIF-1α)检测试剂盒 Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit
HZ-EL-H1278c 人血清淀粉样蛋白A3(SAA3)检测试剂盒 Human SAA3 (Serum Amyloid A3) ELISA Kit
HZ-EL-H1279c 人血清淀粉样蛋白P(SAP)检测试剂盒 Human SAP (Serum Amyloid P Component) ELISA Kit