大鼠白细胞分化抗原3(CD3)检测试剂盒说明上写封闭液用蔗糖溶液,一般是用BSA或酪蛋白的蛋白分子封闭空白位点,蔗糖封闭的原理:
1、大鼠白细胞分化抗原3(CD3)检测试剂盒实验中,蔗糖本身没有封闭作用;
2、封闭液一般是BSA、酪蛋白等或者是Tween等。
3、加蔗糖的主要目的是保护ELISA板子吸附(包被)的蛋白,起到“保护剂”的作用。
4、蔗糖溶液一般在ELISA板子经过BSA封闭后加,当然也有将蔗糖加到封闭液中同时进行处理;那一种更好需要你实验后来验证。但多数选择前者。
封闭剂还可用5%的脱脂乳(市售即可),0.4%的明胶,但是实验表明前者比较好,后者不容易洗干净未结合上的明胶。
大鼠白细胞分化抗原3(CD3)检测试剂盒封闭剂的原理就是与酶联板上未结合抗原或抗体的微孔中用封闭剂封闭上,结合抗体或抗原的时候就不会产生非特异结合了,不会影响实验的准确度。
tween的作用:虽然不能单独封闭,但和BSA在封闭时会起到清洗非特异性结合或结合不牢固的蛋白的作用,假如抗体包被在板上会出现YY-
附件:大鼠白细胞分化抗原3(CD3)检测试剂盒实验中缓冲溶液等试剂的配置
ELISA相关产品,详情见:封闭试剂、终止溶液、封闭肽、ELISA稀释液、ELISA试剂盒、ELISA缓冲液、ELISA洗脱缓冲液
为了保证大鼠白细胞分化抗原3(CD3)检测试剂盒实验的准确性,实验操作中加入的缓冲液和各组分一定要精确,将洗涤液加入酶标板中的时候防止加入的试剂混入另外一个酶标孔,严防交叉污染。
HZ-EL-RB0255c 兔闭锁蛋白/遮光蛋白(OCLN)检测试剂盒 Rabbit OCLN (Occludin) ELISA Kit
HZ-EL-RB0256c 兔原钙黏素γA2(PCDHγA2)检测试剂盒 Rabbit PCDHγA2 (Protocadherin Gamma A2) ELISA Kit
HZ-EL-RB0257c 兔抗心磷脂抗体IgM(ACA-IgM)检测试剂盒 Rabbit ACA-IgM (Anti-Cardiolipin Antibody IgM) ELISA Kit
HZ-EL-RB0258c 兔脑钠素/脑钠肽(BNP)检测试剂盒 Rabbit BNP (Brain Natriuretic Peptide) ELISA Kit
HZ-EL-RB0259c 兔Pdgfa相关蛋白(PDAP1)检测试剂盒 Rabbit PDAP1 (Pdgfa Associated Protein 1) ELISA Kit
HZ-EL-RB0260c 兔肾素(REN)检测试剂盒 Rabbit REN (Renin) ELISA Kit
HZ-EL-RB0263c 兔谷胱甘肽S转移酶pi基因(GSTpi)检测试剂盒 Rabbit GSTpi (Glutathione S Transferases Pi) ELISA Kit
HZ-EL-RB0264c 兔细胞粘附蛋白1(CYTH1)检测试剂盒 Rabbit CYTH1 (Cytohesin 1) ELISA Kit
HZ-EL-RB0265c 兔多药耐药相关蛋白1(MRP1)检测试剂盒 Rabbit MRP1 (Multidrug Resistance Associated Protein 1) ELISA Kit
HZ-EL-RB0266c 兔聚集蛋白聚糖(AGC)检测试剂盒 Rabbit AGC (Aggrecan) ELISA Kit
HZ-EL-RB0267c 兔细丝蛋白A(FLNα)检测试剂盒 Rabbit FLNα (Filamin A, Alpha) ELISA Kit
HZ-EL-RB0268c 兔肝脏/红细胞丙酮酸激酶(PK)检测试剂盒 Rabbit PK (Pyruvate Kinase, Liver And RBC) ELISA Kit
HZ-EL-RB0269c 兔白血病抑制因子(LIF)检测试剂盒 Rabbit LIF (Leukemia Inhibitory Factor) ELISA Kit
HZ-EL-RB0270c 兔氧化低密度脂蛋白(OxLDL)检测试剂盒 Rabbit OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
HZ-EL-RB0271c 兔丛生蛋白(CLU)检测试剂盒 Rabbit CLU (Clusterin) ELISA Kit
HZ-EL-RB0273c 兔粒细胞趋化蛋白2(GCP-2)检测试剂盒 Rabbit GCP-2 (Granulocyte Chemotactic Protein 2) ELISA Kit
HZ-EL-RB0274c 兔中间α球蛋白抑制因子H4(ITIH4)检测试剂盒 Rabbit ITIH4 (Inter Alpha-Globulin Inhibitor H4) ELISA Kit
HZ-EL-RB0277c 兔C-肽(C-P)检测试剂盒 Rabbit C-P (C-Peptide) ELISA Kit
HZ-EL-RB0278c 兔补体因子D(CFD)检测试剂盒 Rabbit CFD (Complement Factor D) ELISA Kit
HZ-EL-RB0279c 兔普列克底物蛋白同源物样结构域家族A成员2(PHLDA2)检测试剂盒 Rabbit PHLDA2 (Pleckstrin Homology Like Domain Family A, Member 2) ELISA Kit
HZ-EL-RB0280c 兔微管蛋白α3C(TUBα3C)检测试剂盒 Rabbit TUBα3C (Tubulin Alpha 3c )ELISA Kit
HZ-EL-RB0282c 兔γ1肌动蛋白(ACTG1)检测试剂盒 Rabbit ACTg1 (Actin Gamma 1) ELISA Kit
HZ-EL-RB0283c 兔肿瘤特异生长因子/肿瘤相关因子(TSGF)检测试剂盒 Rabbit TSGF (Tumor Specific Growth Facter/Tumor Supplied Group of Factor) ELISA Kit
HZ-EL-RB0284c 兔过氧化还原酶5(PRDX5)检测试剂盒 Rabbit PRDX5 (Peroxiredoxin 5) ELISA Kit
HZ-EL-RB0285c 兔半胱氨酸蛋白酶抑制剂(CSTA)检测试剂盒 Rabbit CSTA (Cystatin A) ELISA Kit
HZ-EL-RB0286c 兔嗜环蛋白/亲环素D(CYPD)检测试剂盒 Rabbit CYPD (Cyclophilin D) ELISA Kit
HZ-EL-RB0287c 兔抗磷脂酰丝氨酸抗体(APSA)检测试剂盒 Rabbit APSA (Anti-Phosphatidyl Serine Antibody) ELISA Kit