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大鼠低密度脂蛋白(LDL)ELISA试剂盒
Rat Low density lipoprotein(LDL) FOR RESEARCH USE ONLY Assay range:5μmol/L -160μmol/L 48determinations Purpose This kit allows for the determination of LDL concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat LDL level in the sample, use Purified Rat LDL antibody to coat microtiter plate wells, make solid-phase antibody, then add LDL to wells, Combined LDL antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat LDL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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2015/09/10
大鼠低密度脂蛋白(LDL)ELISA试剂盒
大鼠低密度脂蛋白(LDL)ELISA试剂盒 大鼠低密度脂蛋白(LDL)酶联免疫分析 试剂盒使用说明书 本试剂盒仅供研究使用。 检测范围: 96T 使用目的: 本试剂盒用于测定大鼠血清、血浆及相关液体样本中低密度脂蛋白(LDL)含量。 实验原理 本试剂盒应用双抗体夹心法测定标本中大鼠低密度脂蛋白(LDL)水平。用纯化的大鼠低密度脂蛋白(LDL)抗体包被微孔板,制成固相 抗体,往包被单抗的微孔中依次加入低密度脂蛋白(LDL),再与HRP 标记的低密度脂蛋白(LDL)抗体结合,形成抗体-抗原-酶标抗体复合 物,经过彻底洗涤后加底物TMB 显色。TMB 在HRP 酶的催化下转化成蓝色,并在酸的作 用下转化成最终的黄色。颜色的深浅和样品中的低密度脂蛋白(LDL)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),通过标准曲线计算样品中大鼠低密度脂蛋白(LDL)浓度。
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2014/08/11
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