人甲状腺素(T4)ELISA Kit产品类型:ELISA Kit产品名称:人甲状腺素(T4)ELISA Kit英文名称:Human thyroxine,T4 ELISA Kit人甲状腺素(T4)ELISA Kit规格:96T人甲状腺素(T4)ELISA Kit种属:Human待测物名称:thyroxine,T4缩写:T4蛋白功能1:Thyroid function检测范围:20 ng/ml-320 ng/ml灵敏度:10 ng/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nm人甲状腺素(T4)ELISA KitThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensity of the color is measured.

大鼠P物质受体(SP-R)ELISA Kit产品类型:ELISA Kit产品名称:大鼠P物质受体(SP-R)ELISA Kit英文名称:Rat substance P receptor,SP-R ELISA Kit大鼠P物质受体(SP-R)ELISA Kit别名:NK1R, NKIR, SPR, TAC1R, NK-1 receptor|neurokinin 1 receptor|tachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)|tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)规格:96T大鼠P物质受体(SP-R)ELISA Kit种属:Rat待测物名称:tachykinin receptor 1缩写:TACR1检测范围:37.5 pg/ml-2400 pg/ml灵敏度:9.38 pg/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nm大鼠P物质受体(SP-R)ELISA KitThis gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P.[provided by RefSeq]

疾病：了解风湿性关节炎的发病机制发表的一篇论文提出了微生物感染可能触发风湿性关节炎(一种自体免疫疾病)的一个潜在机制。风湿性关节炎(影响关节的一种慢性病)是当免疫系统攻击身体时出现的。遗传已知在其中起一定作用，免疫系统相关的基因所存在的差异使一个人易患这种病、或保护其不患这种病。以特定“瓜氨酸化”的蛋白为目标的自身抗体(以一个人自己的蛋白为目标的抗体)在风湿性关节炎患者中普遍存在。“瓜氨酸化”的蛋白是名为“精氨酸”的氨基酸已被转化成名为“瓜氨酸”的氨基酸的蛋白。René Toes及同事现在发现，这些“瓜氨酸化”的蛋白中一种被称为“粘着斑蛋白”的蛋白(见于风湿性关节炎患者的关节中)被自身抗体和免疫系统的细胞当成目标。作者发现，免疫反应不会在携带某种保护性基因变异体的风湿性关节炎患者身上发生。他们还发现，T-细胞能够识别“粘着斑蛋白”分子内一个特定的氨基酸序列，该分子也见于很多微生物。这一发现，连同来自以前研究工作的其他方面的证据，说明微生物在该疾病的发病过程中可能扮演一个角色。

 鸡α1酸性糖蛋白(OGCHI)ELISA kit产品类型:ELISA Kit产品名称:鸡α1酸性糖蛋白(OGCHI)ELISA kit英文名称:Chicken alpha 1-acid glycoprotein (OGCHI) ELISA kit货号:鸡α1酸性糖蛋白(OGCHI)ELISA kit别名:Ovoglycoprotein规格:96T中文价格:鸡α1酸性糖蛋白(OGCHI)ELISA kit种属:Chicken待测物名称:alpha 1-acid glycoprotein (OGCHI)缩写:OGCHI蛋白功能1:immunity蛋白功能3:Acute phase检测范围:78 ng/ml-5000 ng/ml灵敏度:19.5ng/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450nmThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with OGCHI. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for 鸡α1酸性糖蛋白(OGCHI)ELISA kitOGCHI. The competitive inhibition reaction is launched between with pre-coated OGCHI and OGCHI in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of OGCHI in the samples. The color development is stopped and the intensity of the color is measured.

大鼠孤腓肽(OFQ/N)ELISA Kit产品类型:ELISA Kit产品名称:大鼠孤腓肽(OFQ/N)ELISA Kit英文名称:Rat orphanin FQ/nociceptin,OFQ/N ELISA Kit货号:大鼠孤腓肽(OFQ/N)ELISA Kit规格:96T中文价格:大鼠孤腓肽(OFQ/N)ELISA Kit种属:Rat待测物名称:orphanin FQ/nociceptin,OFQ/N缩写:OFQ/N检测范围:3.12 pg/ml-200 pg/ml灵敏度:0.78 pg/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OFQ/N has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OFQ/N present is bound by the immobilized antibody. After removing any unbound substances, 大鼠孤腓肽(OFQ/N)ELISA Kita biotin-conjugated antibody specific for OFQ/N is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OFQ/N bound in the initial step. The color development is stopped and the intensity of the color is measured.

常见突变和心脏病相关最近，来自康州大学(University of Connecticut)的研究人员发现，一种调节胆固醇水平的基因的普通突变或许会增加携带突变基因个体的心脏疾病的风险，相关研究刊登于国际杂志PLoS One上。文章中，研究人员对2000至2002年间招募的5000名参与者的研究信息进行了分析，检测了名为错义rs4238001突变体的基因突变，该突变会改变基因SCARB1表达的蛋白的类型；文章中研究者在过去7年间绘制了参与者机体的基因型，并且追踪了参与者心脏病的发作情况。结果发现，这种突变和个体心脏病发作风险增加直接相关，尤其是男性和非洲裔美国人。在携带rs4238001突变体的参与者中，个体的心脏病风险相比一般个体要高出49%；而总的来讲，携带这种突变的男性比不携带的男性患心脏病风险要高29%，而非洲裔的美国男性个体往往可能更糟，其患心脏病的风险会增加49%，白人男性则会增加24%。研究者表示，这种突变并不罕见，对rs4238001 SNP的遗传检测可以帮助临床医生鉴别出那些携带该突变的患者，以便医生们可以为这些携带者提供更好的心脏疾病风险的预防和干预知识。如今研究人员正在通过研究来阐明SCARB1基因突变和心脏疾病发病风险之间的关联，研究者希望可以进行更深入的研究来找到修复细胞中基因突变的方法，从而为降低患者患心脏疾病的风险带来帮助。本文研究或可影响当前预防心脏疾病及基因突变携带患者疗法的护理标准，而研究者指出，我们或许可以通过一些间接手段来有效预防心脏疾病的发生，比如调节患者机体激素水平来改变其胆固醇的代谢等方法。

很多年来研究者们一直认为瘦素抗性是引发肥胖的可能性原因，但近日，来自辛辛那提大学的科学家通过研究却发现，瘦素的作用并不是引发个体肥胖的罪魁祸首，相关研究成果刊登于国际杂志Cell Metabolism上。研究者Diego Perez-Tilve博士表示，恢复瘦素的作用或许并不能有效地减少肥胖，因为瘦素的作用是正常的，而并不是像是在肥胖中受损一样；瘦素是一种激素，其在机体食欲和体重控制上扮演着重要作用，当我们吃饱时瘦素就会产生，随后向大脑发送信号，提示机体已经有足够的能量了，从而大脑就会做出反应减少机体进食。1994年研究者对一系列肥胖小鼠进行研究，发现其机体根本不能产生瘦素，从那时其岁瘦素的研究就一直是一个热点领域；研究者表示，小鼠非常肥胖因为其一直处于饥饿状态，而当利用瘦素处理小鼠后，其就会停止进食，并且开始降低体重。科学家们开始非常好奇，因为相比平均体重的个体而言，肥胖个体机体中的瘦素水平非常高，而研究者从理论上认为机体会制造额外的瘦素来帮助抵御肥胖，而且肥胖个体也需要更多的瘦素来向大脑发送信号从而间接阻断进食。然而在人类临床试验中，研究者发现给予肥胖病人更多的瘦素似乎并没有发挥作用，这些个体依然吃的很多而且依然肥胖，因此肥胖或许并不是机体瘦素耐受的一种状态。这项研究中研究者通过阻断较瘦小鼠及肥胖小鼠机体中的瘦素作用，结果发现两种体重的小鼠依然吃的很多，而且体重增加相同，这就再次证明了瘦素的作用或许在肥胖小鼠中并没有受到损伤。目前肥胖影响着超过三分之一的美国人，而且其严重影响了美国的国家医疗保健系统，本文研究结果告诉我们或许应当重新思考利用瘦素来作用开发治疗肥胖的新型潜在靶点，而不是一味地认为瘦素耐药性是个体肥胖的元凶

 大鼠Kelch重复BTB域包含蛋白10(KBTBD10)ELISA kit产品类型:ELISA Kit产品名称:大鼠Kelch重复BTB域包含蛋白10(KBTBD10)ELISA kit英文名称:Rat Kelch repeat and BTB domain-containing protein 10(KBTBD10) ELISA kit大鼠Kelch重复BTB域包含蛋白10(KBTBD10)ELISA kit别名:SARCOSIN, sarcomeric muscle protein规格:96T大鼠Kelch重复BTB域包含蛋白10(KBTBD10)ELISA kit种属:Rat待测物名称:kelch repeat and BTB (POZ) domain containing 10缩写:KBTBD10蛋白功能1:Ubiquitin蛋白功能3:Ubl conjugation pathway检测范围:15.6 pg/ml-1000 pg/ml灵敏度:3.9 pg/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KBTBD10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KBTBD10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KBTBD10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KBTBD10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

 人Kelch重复和BTB域包含蛋白10(KBTBD10)ELISA kit产品类型:ELISA Kit产品名称:人Kelch重复和BTB域包含蛋白10(KBTBD10)ELISA kit英文名称:Human Kelch repeat and BTB domain-containing protein 10(KBTBD10) ELISA kit人Kelch重复和BTB域包含蛋白10(KBTBD10)ELISA kit别名:SARCOSIN, sarcomeric muscle protein规格:96T人Kelch重复和BTB域包含蛋白10(KBTBD10)ELISA kit种属:Human待测物名称:kelch repeat and BTB (POZ) domain containing 10缩写:KBTBD10蛋白功能1:Ubiquitin蛋白功能3:Ubl conjugation pathway检测范围:25 pg/ml-1600 pg/ml灵敏度:6.25 pg/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KBTBD10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KBTBD10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KBTBD10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KBTBD10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

鸭雌二醇(E2)ELISA Kit产品类型:ELISA Kit产品名称:鸭雌二醇(E2)ELISA Kit英文名称:Duck estradiol (E2) ELISA kit货号:XY-EQ027953DU规格:96T种属:Duck待测物名称:estradiol (E2)缩写:E2蛋白功能1:Sex hormone检测范围:20 pg/ml-1600 pg/ml灵敏度:25 pg/ml反应时间:1-3h所需样本体积:50-100ul检测波长:450nmThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.

鸭雌二醇(E2)ELISA Kit产品类型:ELISA Kit产品名称:鸭雌二醇(E2)ELISA Kit英文名称:Duck estradiol (E2) ELISA kit货号:XY-EQ027953DU规格:96T种属:Duck待测物名称:estradiol (E2)缩写:E2蛋白功能1:Sex hormone检测范围:20 pg/ml-1600 pg/ml灵敏度:25 pg/ml反应时间:1-3h所需样本体积:50-100ul检测波长:450nmThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.

大肠杆菌宿主残留蛋白(E.coli P)ELISA kit产品类型:ELISA kit产品名称:大肠杆菌宿主残留蛋白(E.coli P)ELISA kit英文名称:Escherichia coli protein (E.coli P) ELISA kit货号:XY-E11306o规格:96T种属:Escherichia coli待测物名称:Escherichia coli protein (E.coli P)缩写:E.coli P检测范围:0.156 ng/ml-10 ng/ml灵敏度:0.039 ng/ml反应时间:1-3h所需样本体积:50-100ul检测波长:450nmThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for E.coli P has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any E.coli P present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for E.coli P is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of E.coli P bound in the initial step. The color development is stopped and the intensity of the color is measured.

人EB病毒(EBv)抗体(IgG)ELISA Kit产品类型:ELISA Kit产品名称:人EB病毒(EBv)抗体(IgG)ELISA Kit英文名称:Human epstein-barr virus (EBv)antibody (IgG) ELISA Kit货号:XY-E05010h别名:N/A规格:96T种属:Human待测物名称:epstein-barr virus IgG,EBv IgG缩写:EBv antibody (IgG)蛋白功能1:Infection反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmThis assay employs the qualitative enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with antigen.Samples are pipetted into the wells with anti-human IgG conjugated HorseradishPeroxidase (HRP). Any antibodies specific for the antigen present will bind to thepre-coated antigen. Following a wash to remove any unbound reagent, asubstrate solution is added to the wells and color develops in proportion to theamount of human EBv antibody (IgG) bound in the initial step. The colordevelopment is stopped and the intensity of the color is measured.

粘蛋白5AC(MUC5AC)检测试剂盒适用生物     Homo sapiens (Human，人)    粘蛋白5AC(MUC5AC)检测试剂盒    检测范围     78.13-5000pg/mL     灵敏度     30pg/mL    样本类型     Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     粘蛋白5AC(MUC5AC)检测试剂盒规格     96T    ELISA Kit for Mucin 5 Subtype AC (MUC5AC)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    粘蛋白5AC(MUC5AC)检测试剂盒Sample type     Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     78.13-5000pg/mL The standard curve concentrations used for the ELISA’s were 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312.5pg/mL, 156.25pg/mL, 78.13pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 30pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Mucin 5 Subtype AC (MUC5AC). No significant cross-reactivity or interference between Mucin 5 Subtype AC (MUC5AC) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Mucin 5 Subtype AC (MUC5AC) and the recovery rates were calculated by comparing the measured value to the expected amount of Mucin 5粘蛋白5AC(MUC5AC)检测试剂盒 Subtype AC (MUC5AC) in samples. Matrix     Recovery range (%)     Average(%)    EDTA plasma(n=5)     90-97     93    heparin plasma(n=5)     82-95     87    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mucin 5 Subtype AC (MUC5AC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    EDTA plasma(n=5)     86-96%     81-89%     80-90%     79-101%    heparin plasma(n=5)     88-96%     83-91%     79-101%     94-103%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on 粘蛋白5AC(MUC5AC)检测试剂盒the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in粘蛋白5AC(MUC5AC)检测试剂盒 this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype AC (MUC5AC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype AC (MUC5AC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 5 Subtype AC (MUC5AC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 5 Subtype AC (MUC5AC) in the 粘蛋白5AC(MUC5AC)检测试剂盒samples is then determined by comparing the O.D. of the samples to the standard curve.

Dickkopf相关蛋白1(DKK1)检测试剂盒适用生物     Homo sapiens (Human，人)    Dickkopf相关蛋白1(DKK1)检测试剂盒    检测范围     0.156-10ng/mL     灵敏度     0.058ng/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids    实验时长     4.5h     实验方法     双抗夹心法    Dickkopf相关蛋白1(DKK1)检测试剂盒规格     96T    ELISA Kit for Dickkopf Related Protein 1 (DKK1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Dickkopf相关蛋白1(DKK1)检测试剂盒   Sample type     Serum, plasma, tissue homogenates and other biological fluids    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.058ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 1 (DKK1). No significant cross-reactivity or interference between Dickkopf Related Protein 1 (DKK1) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Dickkopf Related Protein 1 (DKK1) and the recovery ratesDickkopf相关蛋白1(DKK1)检测试剂盒 were calculated by comparing the measured value to the expected amount of Dickkopf Related Protein 1 (DKK1) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     86-101     93    EDTA plasma(n=5)     85-96     90    heparin plasma(n=5)     80-93     82    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dickkopf Related Protein 1 (DKK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dickkopf Related Protein 1 (DKK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayedDickkopf相关蛋白1(DKK1)检测试剂盒 by testing samples spiked with appropriate concentration of Dickkopf Related Protein 1 (DKK1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     81-89%     81-91%     83-97%     83-99%    EDTA plasma(n=5)     78-93%     79-93%     96-104%     89-97%    heparin plasma(n=5)     87-101%     96-105%     93-102%     83-103%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested Dickkopf相关蛋白1(DKK1)检测试剂盒that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 1 (DKK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 1 (DKK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 1 (DKK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. Dickkopf相关蛋白1(DKK1)检测试剂盒The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dickkopf Related Protein 1 (DKK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

环加氧酶2(PTGS2)检测试剂盒适用生物     Homo sapiens (Human，人)    环加氧酶2(PTGS2)检测试剂盒    检测范围     0.313-20ng/mL     灵敏度     0.119ng/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids    实验时长     4.5h     实验方法     双抗夹心法    环加氧酶2(PTGS2)检测试剂盒规格     96T    ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    环加氧酶2(PTGS2)检测试剂盒  Sample type     Serum, plasma, tissue homogenates and other biological fluids    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.119ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Prostaglandin Endoperoxide Synthase 2 (PTGS2). No significant cross-reactivity or interference between Prostaglandin Endoperoxide Synthase 2 (PTGS2) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Prostaglandin Endoperoxide Synthase 2 (PTGS2) and the recovery rates were calculated by comparing the measured value to the expected amount 环加氧酶2(PTGS2)检测试剂盒of Prostaglandin Endoperoxide Synthase 2 (PTGS2) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     95-104     101    EDTA plasma(n=5)     80-92     85    heparin plasma(n=5)     93-101     96    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prostaglandin Endoperoxide Synthase 2 (PTGS2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     89-97%     81-96%     93-101%     98-105%    EDTA plasma(n=5)     89-103%     79-103%     90-97%     84-99%    heparin plasma(n=5)     78-91%     92-102%     82-101%     86-98%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that环加氧酶2(PTGS2)检测试剂盒 the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in 环加氧酶2(PTGS2)检测试剂盒this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Endoperoxide Synthase 2 (PTGS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin Endoperoxide Synthase 2 (PTGS2) in the samples is then determined by comparing the O.D. 环加氧酶2(PTGS2)检测试剂盒of the samples to the standard curve.

烟碱型胆碱受体α1(CHRNα1)检测试剂盒适用生物     Rattus norvegicus (Rat，大鼠)    烟碱型胆碱受体α1(CHRNα1)检测试剂盒    检测范围     0.313-20ng/mL     灵敏度     0.122ng/mL    样本类型     Tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法    烟碱型胆碱受体α1(CHRNα1)检测试剂盒规格     96T    ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Rattus norvegicus (Rat)    烟碱型胆碱受体α1(CHRNα1)检测试剂盒 Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.122ng/mL.    SpecificityThis assay has high sensitivity and excellent 烟碱型胆碱受体α1(CHRNα1)检测试剂盒specificity for detection of Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the烟碱型胆碱受体α1(CHRNα1)检测试剂盒 whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this 烟碱型胆碱受体α1(CHRNα1)检测试剂盒kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. 烟碱型胆碱受体α1(CHRNα1)检测试剂盒The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

前列腺素I合酶(PTGIS)检测试剂盒适用生物     Homo sapiens (Human，人)    前列腺素I合酶(PTGIS)检测试剂盒   检测范围     1.56-100ng/mL     灵敏度     0.67ng/mL    样本类型     Tissue homogenates, cell lysates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     前列腺素I合酶(PTGIS)检测试剂盒规格     96T    ELISA Kit for Prostaglandin I Synthase (PTGIS)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    前列腺素I合酶(PTGIS)检测试剂盒  Sample type     Tissue homogenates, cell lysates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     1.56-100ng/mL The standard curve concentrations used for the ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL, 1.56ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.67ng/mL.    SpecificityThis assay has high sensitivity 前列腺素I合酶(PTGIS)检测试剂盒and excellent specificity for detection of Prostaglandin I Synthase (PTGIS). No significant cross-reactivity or interference between Prostaglandin I Synthase (PTGIS) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin I Synthase (PTGIS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin I Synthase (PTGIS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under前列腺素I合酶(PTGIS)检测试剂盒 appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is 前列腺素I合酶(PTGIS)检测试剂盒Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin I Synthase (PTGIS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin I Synthase (PTGIS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin I Synthase (PTGIS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit 前列腺素I合酶(PTGIS)检测试剂盒a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin I Synthase (PTGIS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

赤霉素(GA)检测试剂盒适用生物     General，通用    赤霉素(GA)检测试剂盒   检测范围     123.46-10000ng/mL     灵敏度     42.9ng/mL    样本类型     Tissue or cell culture supernates.    实验时长     2.5h     实验方法     竞争抑制法     赤霉素(GA)检测试剂盒站    规格     96T    ELISA Kit for Gibberellic Acid (GA)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     General    赤霉素(GA)检测试剂盒 Sample type     Tissue or cell culture supernates.    Format     96-well strip plate    Assay length     2.5 hours    Detection range     123.46-10000ng/mL The standard curve concentrations used for the ELISA’s were 10000ng/mL, 3333.33ng/mL, 1111.11ng/mL, 370.37ng/mL, 123.46ng/mL    Sensitivity     The minimum detectable赤霉素(GA)检测试剂盒 dose of this kit is typically less than 42.9ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Gibberellic Acid (GA). No significant cross-reactivity or interference between Gibberellic Acid (GA) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gibberellic Acid (GA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gibberellic Acid (GA) were tested 赤霉素(GA)检测试剂盒on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the赤霉素(GA)检测试剂盒 performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 50μL standard or sample to each well.    And then add 50μL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37oC;3. Aspirate and wash 3 times;4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;5. Aspirate and wash 5 times;6. Add 90μL Substrate Solution. 赤霉素(GA)检测试剂盒Incubate 15-25 minutes at 37oC;7. Add 50μL Stop Solution. Read at 450 nm immediately.Test principleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gibberellic Acid (GA) has been pre-coated onto a microplate. A competitive inhibition reaction 赤霉素(GA)检测试剂盒is launched between biotin labeled Gibberellic Acid (GA) and unlabeled Gibberellic Acid (GA) (Standards or samples) with the pre-coated antibody specific to Gibberellic Acid (GA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gibberellic 赤霉素(GA)检测试剂盒Acid (GA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gibberellic Acid (GA) in the sample.

钙调磷酸酶(CaN)检测试剂盒适用生物     Rattus norvegicus (Rat，大鼠)    钙调磷酸酶(CaN)检测试剂盒   检测范围     0.313-20ng/mL     灵敏度     0.104ng/mL    样本类型     Tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     钙调磷酸酶(CaN)检测试剂盒规格     96T    ELISA Kit for Calcineurin (CaN)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Rattus norvegicus (Rat)    钙调磷酸酶(CaN)检测试剂盒 Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.104ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcineurin (CaN). No significant cross-reactivity or interference between Calcineurin (CaN) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcineurin (CaN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcineurin (CaN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate 钙调磷酸酶(CaN)检测试剂盒storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is 钙调磷酸酶(CaN)检测试剂盒Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcineurin (CaN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcineurin (CaN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcineurin (CaN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcineurin (CaN) in 钙调磷酸酶(CaN)检测试剂盒the samples is then determined by comparing the O.D. of the samples to the standard curve.

髓样前体细胞抑制因子2(MPIF2)检测试剂盒适用生物     Homo sapiens (Human，人)    髓样前体细胞抑制因子2(MPIF2)检测试剂盒   检测范围     62.5-4000pg/mL     灵敏度     25.5pg/mL    样本类型     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     图表曲线等信息请点击链接到英文官方网站    规格     96T    ELISA Kit for Myeloid Progenitor Inhibitory Factor 2 (MPIF2)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    髓样前体细胞抑制因子2(MPIF2)检测试剂盒 Sample type     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     62.5-4000pg/mL The standard curve concentrations used for the ELISA’s were 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL    Sensitivity     The minimum detectable dose 髓样前体细胞抑制因子2(MPIF2)检测试剂盒of this kit is typically less than 25.5pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Myeloid Progenitor Inhibitory Factor 2 (MPIF2). No significant cross-reactivity or interference between Myeloid Progenitor Inhibitory Factor 2 (MPIF2) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Myeloid Progenitor Inhibitory Factor 2 (MPIF2) and the recovery rates were calculated by comparing the measured value to the expected amount of Myeloid Progenitor Inhibitory Factor 2 (MPIF2) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     79-104     88    EDTA plasma(n=5)     96-104     99    heparin plasma(n=5)     93-102     98    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by 髓样前体细胞抑制因子2(MPIF2)检测试剂盒testing samples spiked with appropriate concentration of Myeloid Progenitor Inhibitory Factor 2 (MPIF2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     78-103%     88-104%     91-98%     86-101%    EDTA plasma(n=5)     91-98%     78-94%     84-99%     91-104%    heparin plasma(n=5)     90-104%     98-105%     97-105%     84-98%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is 髓样前体细胞抑制因子2(MPIF2)检测试剂盒Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Progenitor Inhibitory Factor 2 (MPIF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid髓样前体细胞抑制因子2(MPIF2)检测试剂盒 Progenitor Inhibitory Factor 2 (MPIF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Toll样受体2(TLR2)检测试剂盒适用生物     Rattus norvegicus (Rat，大鼠)    ELISA Kit for Toll Like Receptor 2 (TLR2)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Rattus norvegicus (Rat)    Toll样受体2(TLR2)检测试剂盒  Sample type     Serum, plasma, tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.115ng/mL.    Toll样受体2(TLR2)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 2 (TLR2). No significant cross-reactivity or interference between Toll Like Receptor 2 (TLR2) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Toll Like Receptor 2 (TLR2) and the recovery rates were calculated by comparing the measured value to the expected amount of Toll Like Receptor 2 (TLR2) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     80-98     90    EDTA plasma(n=5)     90-104     94    heparin plasma(n=5)     84-104     101    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Toll Like Receptor 2 (TLR2) and theirToll样受体2(TLR2)检测试剂盒serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     81-103%     79-101%     93-101%     87-102%    EDTA plasma(n=5)     96-103%     95-104%     98-105%     81-103%    heparin plasma(n=5)     83-95%     88-96%     78-99%     89-102%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in thisToll样受体2(TLR2)检测试剂盒kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 2 (TLR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 2 (TLR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 2 (TLR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor 2 (TLR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.   检测范围     0.313-20ng/mL     灵敏度     0.115ng/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     图表曲线等信息请点击链接到英文官方网站    规格     96T    ELISA Kit for Toll Like Receptor 2 (TLR2)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Rattus norvegicus (Rat)    Toll样受体2(TLR2)检测试剂盒 Sample type     Serum, plasma, tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.115ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 2 (TLR2). No significant cross-reactivity or interference between Toll Like Receptor 2 (TLR2) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Toll Like Receptor 2 (TLR2) and the recovery rates were calculated by comparing the measured value to the expected amount of Toll Like Receptor 2 (TLR2) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     80-98     90    EDTA plasma(n=5)     90-104     94    heparin plasma(n=5)     84-104     101    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Toll Like Receptor 2 (TLR2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     81-103%     79-101%     93-101%     87-102%    EDTA plasma(n=5)     96-103%     95-104%     98-105%     81-103%    heparin plasma(n=5)     83-95%     88-96%     78-99%     89-102%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in thisToll样受体2(TLR2)检测试剂盒kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 2 (TLR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 2 (TLR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 2 (TLR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor 2 (TLR2) in theToll样受体2(TLR2)检测试剂盒samples is then determined by comparing the O.D. of the samples to the standard curve.

凋亡信号调节激酶I(ASK1)检测试剂盒适用生物     Homo sapiens (Human，人)    凋亡信号调节激酶I(ASK1)检测试剂盒   检测范围     0.313-20ng/mL     灵敏度     0.115ng/mL    样本类型     Tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     凋亡信号调节激酶I(ASK1)检测试剂盒    规格     96T    ELISA Kit for Apoptosis Signal Regulating Kinase 1 (ASK1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    凋亡信号调节激酶I(ASK1)检测试剂盒 Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.115ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Apoptosis Signal Regulating Kinase 1 (ASK1). No significant cross-reactivity or interference between Apoptosis Signal Regulating Kinase 1 (ASK1) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Signal Regulating Kinase 1 (ASK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Signal Regulating Kinase 1 (ASK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV凋亡信号调节激酶I(ASK1)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 凋亡信号调节激酶I(ASK1)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis Signal Regulating Kinase 1 (ASK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptosis Signal Regulating Kinase 1 (ASK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptosis Signal Regulating Kinase 1 (ASK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptosis Signal Regulating Kinase 1 (ASK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

细胞外信号调节激酶1(ERK1)检测试剂盒适用生物     Homo sapiens (Human，人)    细胞外信号调节激酶1(ERK1)检测试剂盒    检测范围     0.313-20ng/mL     灵敏度     0.114ng/mL    样本类型     Tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     细胞外信号调节激酶1(ERK1)检测试剂盒    规格     96T    ELISA Kit for Extracellular Signal Regulated Kinase 1 (ERK1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    细胞外信号调节激酶1(ERK1)检测试剂盒 Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    细胞外信号调节激酶1(ERK1)检测试剂盒Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.114ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Extracellular Signal Regulated Kinase 1 (ERK1). No significant cross-reactivity or interference between Extracellular Signal Regulated Kinase 1 (ERK1) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Extracellular Signal Regulated Kinase 1 (ERK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Extracellular Signal Regulated Kinase 1 (ERK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.细胞外信号调节激酶1(ERK1)检测试剂盒Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 细胞外信号调节激酶1(ERK1)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Signal Regulated Kinase 1 (ERK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Signal Regulated Kinase 1 (ERK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Signal Regulated Kinase 1 (ERK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Extracellular Signal Regulated Kinase 1 (ERK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

花生四烯酸-5-脂加氧酶(ALOX5)检测试剂盒适用生物     Homo sapiens (Human，人)    花生四烯酸-5-脂加氧酶(ALOX5)检测试剂盒   检测范围     0.156-10ng/mL     灵敏度     0.053ng/mL    样本类型     Tissue homogenates, cell lysates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     花生四烯酸-5-脂加氧酶(ALOX5)检测试剂盒   规格     96T    ELISA Kit for Arachidonate-5-Lipoxygenase (ALOX5)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    花生四烯酸-5-脂加氧酶(ALOX5)检测试剂盒  Sample type     Tissue homogenates, cell lysates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.053ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Arachidonate-5-Lipoxygenase (ALOX5). No significant cross-reactivity or interference between Arachidonate-5-Lipoxygenase (ALOX5) and analogues was observed.花生四烯酸-5-脂加氧酶(ALOX5)检测试剂盒PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arachidonate-5-Lipoxygenase (ALOX5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arachidonate-5-Lipoxygenase (ALOX5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 花生四烯酸-5-脂加氧酶(ALOX5)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-5-Lipoxygenase (ALOX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-5-Lipoxygenase (ALOX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-5-Lipoxygenase (ALOX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-5-Lipoxygenase (ALOX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

基质金属蛋白酶3(MMP3)检测试剂盒适用生物     Oryctolagus cuniculus (Rabbit，兔)    基质金属蛋白酶3(MMP3)检测试剂盒    检测范围     0.156-10ng/mL     灵敏度     0.062ng/mL    样本类型     Serum, plasma and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法    基质金属蛋白酶3(MMP3)检测试剂盒规格     96T    ELISA Kit for Matrix Metalloproteinase 3 (MMP3)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Oryctolagus cuniculus (Rabbit)    Product No.     xyEA101Rb    Sample type     Serum, plasma and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.062ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 3 (MMP3). No significant cross-reactivity or interference between Matrix Metalloproteinase 3 (MMP3) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 3 (MMP3) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 3 (MMP3) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     92-101     96    heparin plasma(n=5)     89-96     92    基质金属蛋白酶3(MMP3)检测试剂盒PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 3 (MMP3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     79-89%     80-98%     93-105%     95-104%    heparin plasma(n=5)     87-96%     91-99%     82-90%     80-97%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 基质金属蛋白酶3(MMP3)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 3 (MMP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 3 (MMP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 3 (MMP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 3 (MMP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

血小板衍生生长因子B(PDGFB)检测试剂盒适用生物     Homo sapiens (Human，人)    血小板衍生生长因子B(PDGFB)检测试剂盒    检测范围     15.63-1000pg/mL     灵敏度     6.3pg/mL    样本类型     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     血小板衍生生长因子B(PDGFB)检测试剂盒规格     96T    ELISA Kit for Platelet Derived Growth Factor Subunit B (PDGFB)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     xyEC921Hu    Sample type     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 6.3pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit B (PDGFB). No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit B (PDGFB) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Platelet Derived Growth Factor Subunit B (PDGFB) and the recovery rates were calculated by comparing the measured value to the expected amount of Platelet Derived Growth Factor Subunit B (PDGFB) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     95-102     99    EDTA plasma(n=5)     87-99     96    heparin plasma(n=5)     93-105     101    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV血小板衍生生长因子B(PDGFB)检测试剂盒LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Platelet Derived Growth Factor Subunit B (PDGFB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     90-101%     83-104%     81-98%     91-101%    EDTA plasma(n=5)     93-101%     83-97%     78-98%     95-105%    heparin plasma(n=5)     97-104%     95-103%     91-98%     87-101%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 血小板衍生生长因子B(PDGFB)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Subunit B (PDGFB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor Subunit B (PDGFB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)检测试剂盒适用生物     Homo sapiens (Human，人)    V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)检测试剂盒    检测范围     0.156-10ng/mL     灵敏度     0.054ng/mL    样本类型     Tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法    V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)检测试剂盒规格     96T    ELISA Kit for V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     xyEB290Hu    Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.054ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC). No significant cross-reactivity or interference between V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVV-Myc骨髓细胞瘤病毒癌基因同源物(MYC)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

纤维蛋白原α(FGα)检测试剂盒适用生物     Homo sapiens (Human，人)    纤维蛋白原α(FGα)检测试剂盒 检测范围     15.63-1000ng/mL     灵敏度     5.9ng/mL    样本类型     Plasma.    实验时长     4.5h     实验方法     双抗夹心法    纤维蛋白原α(FGα)检测试剂盒规格     96T    ELISA Kit for Fibrinogen Alpha (FGa)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEB154Hu    Sample type     Plasma.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     15.63-1000ng/mL The standard curve concentrations used for the ELISA’s were 1000ng/mL, 500ng/mL, 250ng/mL, 125ng/mL, 62.5ng/mL, 31.25ng/mL, 15.63ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 5.9ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Fibrinogen Alpha (FGa). No significant cross-reactivity or interference between Fibrinogen Alpha (FGa) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Fibrinogen Alpha (FGa) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibrinogen Alpha (FGa) in samples. Matrix     Recovery range (%)     Average(%)    sodium citrate plasma(n=5)     83-103     98    纤维蛋白原α(FGα)检测试剂盒PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Alpha (FGa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Alpha (FGa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibrinogen Alpha (FGa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    sodium citrate plasma(n=5)     87-97%     80-90%     78-103%     78-95%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 纤维蛋白原α(FGα)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Alpha (FGa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Alpha (FGa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Alpha (FGa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibrinogen Alpha (FGa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

磷脂酶A1(PLA1)检测试剂盒适用生物     Homo sapiens (Human，人)    磷脂酶A1(PLA1)检测试剂盒   检测范围     0.156-10ng/mL     灵敏度     0.061ng/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     磷脂酶A1(PLA1)检测试剂盒规格     96T    ELISA Kit for Phospholipase A1 (PLA1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEB151Hu    Sample type     Serum, plasma, tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.061ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A1 (PLA1). No significant cross-reactivity or interference between Phospholipase A1 (PLA1) and analogues was observed.磷脂酶A1(PLA1)检测试剂盒RecoveryMatrices listed below were spiked with certain level of recombinant Phospholipase A1 (PLA1) and the recovery rates were calculated by comparing the measured value to the expected amount of Phospholipase A1 (PLA1) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     96-105     101    EDTA plasma(n=5)     79-98     91    heparin plasma(n=5)     96-104     99    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A1 (PLA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A1 (PLA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Phospholipase A1 (PLA1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     94-104%     88-101%     89-103%     79-94%    EDTA plasma(n=5)     94-103%     99-105%     78-103%     80-96%    heparin plasma(n=5)     96-104%     88-95%     79-91%     84-105%    磷脂酶A1(PLA1)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 磷脂酶A1(PLA1)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A1 (PLA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A1 (PLA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A1 (PLA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A1 (PLA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.