Dickkopf相关蛋白1(DKK1)检测试剂盒
适用生物 Homo sapiens (Human,人)
Dickkopf相关蛋白1(DKK1)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.058ng/mL
样本类型 Serum, plasma, tissue homogenates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法 Dickkopf相关蛋白1(DKK1)检测试剂盒
规格 96T
ELISA Kit for Dickkopf Related Protein 1 (DKK1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Dickkopf相关蛋白1(DKK1)检测试剂盒
Sample type Serum, plasma, tissue homogenates and other biological fluids
Format 96-well strip plate
Assay length 4.5 hours
Detection range 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.058ng/mL.
Specificity
This assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 1 (DKK1).
No significant cross-reactivity or interference between Dickkopf Related Protein 1 (DKK1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Dickkopf Related Protein 1 (DKK1) and the recovery ratesDickkopf相关蛋白1(DKK1)检测试剂盒 were calculated by comparing the measured value to the expected amount of Dickkopf Related Protein 1 (DKK1) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5) 86-101 93
EDTA plasma(n=5) 85-96 90
heparin plasma(n=5) 80-93 82
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dickkopf Related Protein 1 (DKK1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dickkopf Related Protein 1 (DKK1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayedDickkopf相关蛋白1(DKK1)检测试剂盒 by testing samples spiked with appropriate concentration of Dickkopf Related Protein 1 (DKK1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-89% 81-91% 83-97% 83-99%
EDTA plasma(n=5) 78-93% 79-93% 96-104% 89-97%
heparin plasma(n=5) 87-101% 96-105% 93-102% 83-103%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested Dickkopf相关蛋白1(DKK1)检测试剂盒that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B 1×120μL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50μL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 1 (DKK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 1 (DKK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 1 (DKK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. Dickkopf相关蛋白1(DKK1)检测试剂盒The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dickkopf Related Protein 1 (DKK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
更多
实战分享|小鼠淋巴细胞的提取和分选之经验小结
厂商
2023.08.21
实战分享 | 原生细胞的合成之经验分享
厂商
2023.07.28
实验常用人肝癌细胞株(HepG2/Hep3B,HuH-7,MHCC97H,PLC/PRF/5)怎么选
厂商
2023.07.03
原代细胞的培养方法及注意事项
厂商
2023.06.26