Enzyme Linked Immunosorbent Assay

Enzyme Linked Immunosorbent Assay Rabbit Tumor Necrosis Factor α Collect Sample – serum or blood plasma Storage: 2 - 8 °C Package size: 96 determinations PRINCIPLE OF THE METHOD： The TNF-α kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known TNF-α concentrations and unknowns are pipetted into these wells. During the first incubation, the TNF-α antigen and a biotinylated monoclonal antibody specific for TNF-α are simultaneously incubated.Afterwashing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-α present in the samples. REAGENTS PROVIDED AND RECONSTTTUTION： REAGENTS（Store at 2-8℃） 1×96 WELLS 0.5×96 WELLS 96/48-wells microtiter plates 1 0.5 Plastiv cover 2 1 Standard: 400pg/ml 1Vials (1.0ml) 0.5Vials (0.5ml) Blank control 1Vials (1.0ml) 1Vials (0.5ml) Standard Diluent 1Vials (8.0ml) 1Vials (4.0ml) Biotinylated anti-TNF-α 1Vials (8.0ml) 1Vials (4.0ml) Streptavidin-HRP 1Vials (12ml) 1Vials (6.0ml) Washing Buffer 1Vials (20ml) 1Vials (10ml) Substrate A 1Vials (6.0ml) 1Vials (3.0ml) Substrate B 1Vials （6.0ml) 1Vials (3.0ml) Stopping Solution 1Vials (6.0ml) 1Vials (3.0ml) MATERIAL REQUIRED BUT NOT PROVIDED： ? Distilled water ? Pipettes:10ul、50ul、100ul、200ul、1000ul。 ? Vortex mixer and magnetic stirrer.