产品名称: 人维生素D受体(VD R)ELISA Kit
英文名称: Human Vitamin D Receptor,VD R ELISA Kit
货号: CSB-E05136h
规格: 96T
种属: Human
待测物名称: vitamin D (1,25- dihydroxyvitamin D3) receptor
缩写: VDR
蛋白功能1: Transcription/Transcription regulation
蛋白功能3: Transcription
样本类型: serum, plasma, tissue homogenates, cell lysates
检测范围: 6.25 pg/ml-400 pg/ml
灵敏度: 1.56 pg/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VDR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VDR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VDR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VDR bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Human VDR. No significant cross-reactivity or interference between Human VDR and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the VDR concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
相关产品:
产品名称 英文名称 货号 规格
Chicken Vitamin D3 receptor(VDR) ELISA kit Chicken Vitamin D3 receptor(VDR) ELISA kit CSB-EL025832CH 96T
Rat Vitamin D3 receptor(VDR) ELISA kit Rat Vitamin D3 receptor(VDR) ELISA kit CSB-EL025832RA 96T
Pig Vitamin D3 receptor(VDR) ELISA kit Pig Vitamin D3 receptor(VDR) ELISA kit CSB-EL025832PI 96T
Mouse Vitamin D3 receptor(VDR) ELISA kit Mouse Vitamin D3 receptor(VDR) ELISA kit CSB-EL025832MO 96T
Bovine Vitamin D3 receptor(VDR) ELISA kit Bovine Vitamin D3 receptor(VDR) ELISA kit CSB-EL025832BO 96T
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Xenopus laevis Vitamin D3 receptor(vdr) CSB-EP025832XBE 1mg E.coli
Recombinant Xenopus laevis Vitamin D3 receptor(vdr) CSB-YP025832XBE 1mg Yeast
Recombinant Coturnix coturnix japonica Vitamin D3 receptor(VDR) CSB-EP025832DXJ 1mg E.coli
Recombinant Coturnix coturnix japonica Vitamin D3 receptor(VDR) CSB-YP025832DXJ 1mg Yeast
Recombinant Chicken Vitamin D3 receptor(VDR) CSB-EP025832CH 1mg E.coli
Recombinant Chicken Vitamin D3 receptor(VDR) CSB-YP025832CH 1mg Yeast
Recombinant Pig Vitamin D3 receptor(VDR) CSB-EP025832PI 1mg E.coli
Recombinant Pig Vitamin D3 receptor(VDR) CSB-YP025832PI 1mg Yeast
Recombinant Mouse Vitamin D3 receptor(Vdr) CSB-EP025832MO 1mg E.coli
Recombinant Mouse Vitamin D3 receptor(Vdr) CSB-YP025832MO 1mg Yeast
Recombinant Rat Vitamin D3 receptor(Vdr) CSB-EP025832RA 1mg E.coli
Recombinant Rat Vitamin D3 receptor(Vdr) CSB-YP025832RA 1mg Yeast
Recombinant Human Vitamin D3 receptor(VDR) CSB-EP025832HU 1mg E.coli
Recombinant Human Vitamin D3 receptor(VDR) CSB-YP025832HU 1mg Yeast
人维生素D受体(VD R)ELISA Kit由上海希美化学有限公司为您提供,货号CSB-E05136h,规格:96T,CAS号:CSB-E05136h,如您想了解更多关于人维生素D受体(VD R)ELISA Kit价格、人维生素D受体(VD R)ELISA Kit结构式、批发、用途等信息,欢迎咨询。除供应人维生素D受体(VD R)ELISA Kit外,还可为您提供大鼠维生素D(VD)ELISA Kit、鸡维生素D结合蛋白(VDBP)ELISA kit、人维生素D结合蛋白(DBP)ELISA Kit等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海希美化学客户服务电话,售前、售后均可联系。