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当前位置: 上海希美化学 > ELISA试剂盒 > 鸡维生素D结合蛋白(VDBP)ELISA kit

鸡维生素D结合蛋白(VDBP)ELISA kit

供货周期: 一周
品牌: Cusabio
规格: 96T
货号: CSB-EL009306CH
CAS号: CSB-EL009306CH
报价: ¥1
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产品介绍

 产品名称: 鸡维生素D结合蛋白(VDBP)ELISA kit
 
 英文名称: Chicken vitamin D-binding protein,VDBP ELISA kit
 
 别名: DBP, DBP/GC, GRD3, VDBG, VDBP
 
 货号: CSB-EL009306CH
 
 缩写: GC
 
 蛋白功能1: Transport
 
 蛋白功能3: transport
 
 样本类型: serum, plasma, tissue homogenates
 
 灵敏度: 250 ng/ml
 
 反应时间: 1-5h
 
 所需样本体积: 50-100ul
 
 检测波长: 450nm
 
 鸡维生素D结合蛋白(VDBP)ELISA kit

 用途: For research use only. Not for diagnostic use.
 
 鸡维生素D结合蛋白(VDBP)ELISA kit

原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GC bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 鸡维生素D结合蛋白(VDBP)ELISA kit

特异性: This assay has high sensitivity and excellent specificity for detection of GC. No significant cross-reactivity or interference between GC and analogues was observed. 
 鸡维生素D结合蛋白(VDBP)ELISA kit

精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 鸡维生素D结合蛋白(VDBP)ELISA kit

样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4&deg;C before centrifugation for 15 minutes at 1000 &times;g. Remove serum and assay immediately or aliquot and store samples at -20&deg;C or -80&deg;C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 &times;g at 2-8&deg;C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20&deg;C or -80&deg;C. Avoid repeated freeze-thaw cycles.
 
 鸡维生素D结合蛋白(VDBP)ELISA kit

检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4&deg;C.
3. Add 100&mu;l of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37&deg;C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100&mu;l of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37&deg;C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200&mu;l) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100&mu;l of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37&deg;C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90&mu;l of TMB Substrate to each well. Incubate for 15-30 minutes at 37&deg;C. Protect from light.
10. Add 50&mu;l of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 鸡维生素D结合蛋白(VDBP)ELISA kit

结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the GC concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
鸡维生素D结合蛋白(VDBP)ELISA kit

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鸡维生素D结合蛋白(VDBP)ELISA kit由上海希美化学有限公司为您提供,货号CSB-EL009306CH,规格:96T,CAS号:CSB-EL009306CH,如您想了解更多关于鸡维生素D结合蛋白(VDBP)ELISA kit价格、鸡维生素D结合蛋白(VDBP)ELISA kit结构式、批发、用途等信息,欢迎咨询。除供应鸡维生素D结合蛋白(VDBP)ELISA kit外,还可为您提供人维生素D受体(VD R)ELISA Kit、人抗染色质解旋酶DNA结合蛋白4(CHD4)抗体ELISA kit、小鼠卷曲螺旋域结合蛋白80(CCDC80)ELISA kit等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海希美化学客户服务电话,售前、售后均可联系。
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