毛细管液相色谱分析微量生物样品
2008-04-15 12:04
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资料摘要:
Analysis of limited-volume or limited- mass samples introduces an additional variable for the analytical chemist attempting to select the overall “best” method. While traditional LC has the precision, accuracy, and sensitivity characteristics that would be suitable for the vast majority of analyses, it requires injection volumes which might be several percent of the total sample volume available, especially in the case of experimental biologic compounds. Capillary LC, by comparison, requires a significantly smaller injection volume ( 20-200 nL), but total sample volume requirements are not straightforward due to injection complications which are encountered on the microscale. This presentation will attempt to define usable lower volume/mass limits for biologic samples that can be reliably used for analysis; first for a set of model set of phenones and then for peptide and protein mixtures.
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连载系列文章——“液相色谱故障排除”,来自于德怀特·R·斯托尔博士在《LCGC》“液相色谱故障排除”专栏发布的系列文章,由月旭公众号翻译整理。
我们即将发布一系列关于“液相色谱故障排除”的连载文章,来自于德怀特·R·斯托尔博士在《LCGC》“液相色谱故障排除”专栏发布的系列文章,由月旭公众号翻译整理。
目前,在实际工作中使用得较多的核壳型色谱填料,主要有亚-3μm及亚-2μm两种,下面我们将分别进行讨论。
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