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Cryoplunge is a cryo-fixation device to allow rapid freezing of thin film suspensions for cryo-TEM. When used in conjunction with a cryo-transfer system for TEM e.g. CT3500 Cryotrans, Cryoplunge enables reproducible vitreous preparations of biological suspensions. These include viruses, liposomes, bacteriophages, isolated macromolecules, macromolecular assemblies and 2-D crystals.
Cryoplunge utilises the well-proven technique of freezing thin film specimens by plunging into liquid ethane, cooled by liquid nitrogen in a special workstation.
The usefulness of the technique is extended with a timed blotting function to control film thickness before plunging.
Safety is key to the design of Cryoplunge, and following blotting, the opening of a shutter and the spring-assisted release of the forceps are interlocked to a safety guard.
Due to the flammability of ethane vapour, Cryoplunge is entirely controlled by pneumatics, making the apparatus safe to use within a spark-proof fume hood.
Cryoplunge cryo-fixation device, including
pneumatic plunge mechanism and shutter
humidity chamber
timed blotting device
pneumatic system controller
freezing station
grid holder,
transfer container and tools
ethane temperature monitor and alarm
Important: to be operated within a spark-proof fume hood (not supplied)
Options
CPHM, Humidity meter, provides temperature and humidity readout
from Cryoplunge humidity chamber
