赛默飞LTQ Orbitrap XL™组合式质谱仪

碎片离子检索功能在士的宁代谢物鉴定中的应用

马钱子主要有效成分士的宁（strychnine）又名番木鳖碱，属于中枢神经兴奋剂，临床用于偏瘫、瘫痪及因注射链霉素引起的骨骼肌松弛、弱视症等和巴比妥类中毒等。此外，添加士的宁的饲料诱饵可用来消灭老鼠等啮齿类有害动物，然而这些动物体内残留士的宁会通过食物链传递, 对整个生态圈造成影响，故有必要对其代谢产物进行深入研究，为临床用药、环保、司法等部门提供可靠的分析手段和数据。 LTQ Orbitrap XL结合了线性离子阱和Orbitrap高分辨质量分析器，具有高灵敏度的多级高分辨能力。Mass Frontier为Thermo专业的结构解析软件，利用其丰富的碎裂数据库，可快速的对士的宁多级碎裂规律进行准确推测和归属，碎片离子检索功能将母药与代谢物相关性与可能的代谢途径有机的结合起来，从繁杂的基质样品质谱信号中准确的筛选到未知代谢物信息，进一步对代谢位点进行推测，最终获得全面的代谢物信息。

LTQ-Orbitrap组合式高分辨质谱对婴幼儿奶粉中激素类物质多残留的快速筛选和确证

在本试验中，应用液相色谱联用LTQ Orbitrap组合式高分辨质谱仪配合ToxID筛查软件，对婴幼儿奶粉中6种ppb级激素类组分进行快速筛查，在准确质量确证的同时实现了准确定量检测，结果表明，在奶粉复杂基质中，低至1ppb的添加浓度，可全部被准确筛选确证（质量准确度均在3ppm以内）

LTQ-Velos-Pro-Orbitrap-Elite在磷酸化蛋白质组学中的应用

从上述结果可以得出，LTQ-Velos-Pro-Orbitrap-Elite检测结果可靠性高，系统稳定性高，质谱质量轴稳定性高，在短时间内可以鉴定到大量高可信的磷酸化修饰肽段以及磷酸化位点，可以很好的应用于磷酸化蛋白质组学研究，同时本次实验还说明对样本使用TiO2进行多次重复富集可以显著提高样本中磷酸化肽段的比例，提高磷酸化肽段与位点的鉴定数目。

LTQ鉴定和研究人肥厚型心肌病的假定和已知血浆生物标记物及其差异表达

This workflow involved the analysis of a highly complex protein digest and was dependent upon an LC/MS methodology that achieves the best possible peptide detection and analysis, in order to detect important, low-level protein components. In addition to this, many of the peaks used for quantification were small, but accurate comparisons were possible due to the large number of data points defining these peaks. The LTQ acquires five MS or MS/MS spectra/second, ensuring the best possible data for accurate analytical determinations such as these. Up-Regulation of CRP, as measured by a 2.2-fold increase in its raw MS signal (concentration) at T24 vs T0, is consistent with the observed general elevation of acutephase response factors of which it is a part. Future work will involve validation of unique putative markers that were also identified in this analysis.