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Product Description |
| The technique of micro-mirror TIRF (total internal reflection fluorescence) microscopy is the only proven method to study the ordered assembly and function of multi-component biomolecular machines. The Mad City Labs' MicroMirror TIRF system is part of our single molecule imaging instrument portfolio and draws on our expertise with high precision, high stability nanopositioning systems and microscopy solutions. The MicroMirror TIRF system uses through-the-objective excitation, but replaces the dichroic used in conventional TIRF systems with two broadband micro-mirrors positioned at the back aperture of the objective lens. The elimination of the dichroic mirror leads to superior signal-to-noise ratios and simplifies the introduction of multiple spectral lines to your experiment. Typical biomolecular systems studied using micro-mirror TIRF incorporate 3 or more laser lines which can be difficult to accomodate in conventional dichroic TIRF instruments. Each micro-mirror is mounted to a multi-axis precision mount allowing the user to make fine adjustments to the optical pathway. The imaging and motion control platform is designed to maximize optical pathway accessibility and flexibility, while also simplifying the optical alignment. A compelling advantage of our MicroMirror TIRF system is the open access to the entry and exit optical pathways. The motion control platform comprises an XY micropositioning platform with an integrated XYZ closed loop nanopositioning system. The XYZ closed loop nanopositioning uses proprietary PicoQ® sensors for sub-nanometer precision and high stability. A z-axis micropositioner with integrated sample holder is mounted above the nanopositioning system. Completing our MicroMirror TIRF system is the TIRF Lock™ feedback control consisting of a QPD sensor, TIRF Lock™ controller and software. TIRF Lock™ maintains the TIR signal through software feedback to the nanopositioner. The MicroMirror TIRF system is an enabling technology for advanced study of complex biomolecular interactions. The MicroMirror TIRF is a proven design and offers a simple yet flexible instrument platform for single molecule research. Compatible Software Packages:      |
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Technical Specifications - Imaging & Motion Control | |
| Micropositioning motion | XYZ |
| Ranges of motion (XY) | 25 mm |
| Range of motion (Z) | 10 mm |
| Body Material | Anodized Aluminum |
| Nanpositioning Motion | XYZ |
| Ranges of motion (XYZ) | 200 μm |
| Resolution | 0.4 nm |
| Recommended max. load (horizontal)* | 0.5 kg |
| Body Material | Aluminum |
| Controller? | Nano-Drive® |
| Digital Interface | USB 2.0 |
| Analog Input | 0 to 10V |
| Synchronization outputs | TTL (4) |
| Nanopositioner Software? | Nano-Route®3D |
| TIRF Software compatibility | GLIMPSE |
Technical Specifications - MicroMirror Mounts | |
| Micro-Mirror motion | 5 axis |
| Micro-Mirror diameter | 3 mm |
| Mount material | Aluminum |
Technical Specifications - TIRF Lock™ Feedback | |
| TIRF-Lock™ Sensor | Quadrant Photodiode |
| Controller | TIRF Lock |
| Digital interface | USB 2.0 |
| Software | TIRF Lock |
* Larger load requirements should be discussed with our engineering staff. | |
| ? See Software Compatibility, above | |
Additional Information | |
MicroMirror TIRF Catalog Pages | Design and construction of a multiwavelength, micromirror total internal reflectance fluorescence microscope. Larson, J. et al. Nature Protocols 9, 2317–2328 (2014) |
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