本系统称为交钥匙工程的单分子测试全内反射荧光显微镜：
包括：
荧光显微镜；
基于光导的TIRFM 全内反射荧光 显微镜流体系统；
弱光测试的EM CCD相机；
PC控制的多色照明器；
稳压和波函数发生器；
用于仪器控制，数据采集和分析的软件；
TIRF Technologies offers lightguide- and prism-based TIRF Microscopy flow systems (lgTIRFM) equipped with open perfusion chambers and closed flow cells. The advantages of lgTIRFM, with respect to through-objective TIRFM, include superior signal-to-noise ratio, reproducibility of intensity of the evanescent wave between experiments, and compatibility of lgTIRFM with different objectives and illuminators. Oil and water immersion lenses as well as dry objectives can be used with lgTIRFM systems. For example, the same specimen can be imaged in TIRF mode with a 100X oil immersion lens and dry 2X objective to obtain a panoramic view. lgTIRFM generates wide-field (approx 1cm X 1cm) vanescent wave useful for single molecule detection studies, microarray experiments, as well as for TIRF imaging of a group of interacting mammalian cells. lgTIRFM is compatible with inexpensive LED, halogen, xenon-, and mercury-arc lamps, and can also be used with lasers. This allows for excitation of a full spectrum of fluorophores, as opposed to the limited selection matched to lasers. lgTIRFM is ideally suited to studies of surface based molecular-cellular and molecular-molecular interactions. Such studies include single molecule detection, endo- and exocytosis, membrane trafficking, ion-channel applications, protein-protein interactions, interfacial DNA hybridization, etc.
Optional modules for lgTIRFM include: electrochemical, electric field and dielectrophoretic control, computer-controlled fluidics, and low light EM CDD cameras. Modular design of hardware and software enables different configurations of TIRFM systems (from manual to fully automated) that suit wide range of research tasks.
