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Aspergillus brasiliensis Varga et al. 拉丁名
ATCC CRM-16404 编号
Deposited As Aspergillus niger van Tieghem, anamorph
Strain Designations 菌种别名 WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275]
Application 用途
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.
assay of antimicrobial preservatives
media testing
preparatory test control
quality control strain
sterility testing
testing
testing fungicides
transformation host
validation control
Antimicrobial effectiveness testing Ref
Microbial enumeration testing Ref
This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified.
Biosafety Level 安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Type Strain 模式菌株 no
Intended Use
Certified Reference Material produced under an ISO 17034 accredited process.
Preceptrol? no
Comments Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.
Certified Reference Material produced under an ISO 17034 accredited process.
This strain was recently re-named as Aspergillus brasiliensis according to the reference Varga et al. 2007.
Use of impedance for preservative efficacy testing
Morphology Colonies initially white or yellowish, mycelium growing rapidly (to cover plate in 5 to 10 days), soon producing dense layer of erect smooth-stiped, thick-walled conidiophores terminated by globose vesicles bearing phialides (uniseriate) or (commonly) metulae with phialides (biseriate) which produce dry chains of conidia. Revers of plate pale to grayish or greenish yellow, often showing radiating ridges in mycelium. Spore heads radiate, sometimes dividing into columns with age, initially pale, becoming dark brown to black. Individual conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) micrometers in diameter.
Medium 培养基 ATCC? Medium 336: Potato dextrose agar (PDA)
ATCC? Medium 325: Malt extract agar (Blakeslee's formula)
ATCC? Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions 生长条件
Temperature 培养温度 : 24°C to 30°C
Atmosphere 需氧情况 : Typical aerobic
Morphology Colonies initially white or yellowish, mycelium growing rapidly (to cover plate in 5 to 10 days), soon producing dense layer of erect smooth-stiped, thick-walled conidiophores terminated by globose vesicles bearing phialides (uniseriate) or (commonly) metulae with phialides (biseriate) which produce dry chains of conidia. Revers of plate pale to grayish or greenish yellow, often showing radiating ridges in mycelium. Spore heads radiate, sometimes dividing into columns with age, initially pale, becoming dark brown to black. Individual conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) micrometers in diameter.
Name of Depositor 寄存者 SM Ringel
Isolation 分离基物 Blueberry, North Carolina, USA
Cross References
Nucleotide (GenBank) : KU729174 D1/D2 region of 28S rRNA gene
Nucleotide (GenBank) : FJ195348 ITS including 5.8S rRNA gene
References 参考文献
Connolly P, et al. The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products. J. Appl. Bacteriol. 76: 68-74, 1994. PubMed: 8144407
Wnendt S, et al. Molecular cloning, sequence analysis and expression of the gene encoding an antifungal-protein from Aspergillus giganteus. Curr. Genet. 25: 519-523, 1994. PubMed: 8082203
ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA
British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 2003
British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003
British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI C, 2003
Wnendt S, et al. Characterization of the gene encoding alpha-sarcin, a ribosome- inactivating protein secreted by Aspergillus giganteus. Gene 124: 239-244, 1993. PubMed: 8444347
Shen LL, et al. DNA topoisomerases from pathogenic fungi: targets for the discovery of antifungal drugs. Antimicrob. Agents Chemother. 36: 2778-2784, 1992. PubMed: 1336349
European Pharmacopoeia Commission Efficacy of antimicrobial preservation. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.3, 1997
U.S. Pharmacopeia General Chapters:<51> ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011
U.S. Pharmacopeia General Chapters: <71> STERILITY TESTS. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011
U.S. Pharmacopeia Dietary Supplement Chapters: <2021> Microbial Enumeration Tests-Nutritional and Dietary Supplements. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Moreira MT, et alEffect of pulsation on morphology of Aspergillus niger and Phanerochaete chrysosporium in a fluidized-bed reactorIn: Moreira MT, et alImmobilized cells: basics and applicationsAmsterdamElsevierpp. 518-523, 1996
Capobianco JO, et al. Cellular accumulation, localization, and activity of a synthetic cyclopeptamine in fungi. Antimicrob. Agents Chemother. 42: 389-393, 1998. PubMed: 9527791
FF MicroPlate. Biolog.
Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10.
Sterility test, Growth promotion test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.54.
