中科院武汉病毒所安装的CRYOARM 300 (JEM-Z300FSC)为JEOL在国内安装的第一台300 kV冷冻电镜，其配置了冷场发射电子枪、欧米茄能量过滤器和无孔相位板。该电镜已经投入运行约4年，目前产出的数据已经相继发表在包括Nature, Nature Microbiology, Cell Research, Nucleic Acids Research, Nature Communications等期刊上。

1. Yu, G., Wang, X., Zhang, Y. et al. Structure and function of a bacterial type III-E CRISPR–Cas7-11 complex. Nat Microbiol 7, 2078–2088 (2022). https://doi.org/10.1038/s41564-022-01256-z

2. Yang, S., Wang, Y., Yu, F. et al. Structural and functional insights into the modulation of T cell costimulation by monkeypox virus protein M2. Nat Commun 14, 5186 (2023).https://doi.org/10.1038/s41467-023-40748-2

3. Li, Z., Xia, H., Rao, G. et al. Cryo-EM structures of Banna virus in multiple states reveal stepwise detachment of viral spikes. Nat Commun 15, 2284 (2024). https://doi.org/10.1038/s41467-024-46624-x

4. Jiayi Yin, Yan Fu, Guibo Rao, Zhiqiang Li, Kexing Tian, Tingting Chong, Kai Kuang, Manli Wang, Zhihong Hu, Sheng Cao, Structural transitions during the cooperative assembly of baculovirus single-stranded DNA-binding protein on ssDNA, Nucleic Acids Research, Volume 50, Issue 22, 9 December 2022, Pages 13100–13113,

https://doi.org/10.1093/nar/gkac1142

5. Xiaoshen Wang, Guimei Yu, Yanan Wen, Qiyin An, Xuzichao Li, Fumeng Liao, Chengwei Lian, Kai Zhang, Hang Yin, Yong Wei, Zengqin Deng, Heng Zhang, Target RNA-guided protease activity in type III-E CRISPR–Cas system, Nucleic Acids Research, Volume 50, Issue 22, 9 December 2022, Pages 12913–12923, 

https://doi.org/10.1093/nar/gkac1151

6. Wang, X., Li, X., Yu, G. et al. Structural insights into mechanisms of Argonaute protein-associated NADase activation in bacterial immunity. Cell Res 33, 699–711 (2023).

https://doi.org/10.1038/s41422-023-00839-7

7. An, Q., Wang, Y., Tian, Z. et al. Molecular and structural basis of an ATPase-nuclease dual-enzyme anti-phage defense complex. Cell Res 34, 545–555 (2024). https://doi.org/10.1038/s41422-024-00981-w

JEOL新一代300kV冷冻电镜

目前,市场在售的是JEOL新一代300kV冷冻电镜CRYOARM 300II (JEM-3300),其特点如下:

• 高稳定性冷场枪

• 科勒照明模式，消除光斑边缘菲涅尔条纹，提升采集效率

• 低慧差光路系统，大范围移动电子束时光束质量稳定

• 镜筒内置的Omega能量过滤器，一键实现零峰校正
  

• 高真空样品存储仓，换样灵活

图2 CRYOARM 300II外观

相关产品 https://www.instrument.com.cn/netshow/SH100507/C283127.htm