2023/03/03 13:13
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产品配置单:
博奥思 双侧臂细胞培养瓶
型号: B356876
产地: 荷兰
品牌: 博奥思
¥5000 - 2万
参考报价
联系电话
博奥思 BH200-C1低速生物培养磁力搅拌器
型号: BH200-C1
产地: 荷兰
品牌: 博奥思
¥2.5万 - 3.6万
参考报价
联系电话
博奥思 BIOMIX drive 1/ drive 4分体式细胞培养磁驱搅拌器
型号: BIOMIX drive 1/ drive 4
产地: 荷兰
品牌: 博奥思
¥1万 - 5万
参考报价
联系电话
博奥思 Celrol Pro 滚瓶培养装置
型号: Celrol Pro
产地: 荷兰
品牌: 博奥思
¥15万 - 20万
参考报价
联系电话
博奥思 BCI-850C 细胞工厂 / CO2 培养箱
型号: BCI-850C
产地: 荷兰
品牌: 博奥思
¥14万 - 21.5万
参考报价
联系电话
博奥思 B3 / B5 / B7 通用振荡培养箱
型号: B7
产地: 荷兰
品牌: 博奥思
¥10万 - 30万
参考报价
联系电话
博奥思 Microtron 高速振荡培养箱
型号: Microtron
产地: 荷兰
品牌: 博奥思
¥30万 - 45万
参考报价
联系电话
方案详情:
发酵罐在酵母菌的分批培养中的应用
分批反应操作是指基质灭菌、接种以后,除了好氧反应需要在反应过程中通入无菌空气、消除泡沫所用的消泡剂以及维持一定pH值所用酸碱之外,反应过程中不再加入反应基质。
基质浓度、产物浓度以及细胞浓度均随反应进行的时间而变化,尤其是菌体本身将经历不同的生长阶段(在培养的过程中,微生物的生长可分为迟缓期、对数生长期、减速期、静止期),显示出不同的催化活力。
基本特征是:反应物料一次加入一次卸出;反应器物系的组成仅随时间而变化,即随着培养的进行,基质的浓度下降,菌体量增加,产物增加。因此属于一非稳态过程。
Yeast culture –batch and with feeding.
The fermenter is supplied in a configuration to provide an excellent bioreactor for this purpose.
* Powerful, top drive system with lubricated mechanical seal (magnetic options for Techfors)
* Jacketed system for efficient heat transfer.
* High-accuracy pumps for addition of pH control reagents, antifoam and feed (option on some units)
* Control of dissolved oxygen by stirrer speed as standard (air flow, pressure & oxygen, supplement options)
* Optional analogue feed pump for variable speed additon
* Analogue input/output for addition of external pumpsand sensros for eg. OD and Methanol
Vessels:
All vessels are stainless steel (1.4435) with a dished base and top plate in several standard sizes. Temperature control can be by water circulation with either a heating element or steam injection into a pocket/exchanger.
All vessels are with a mechanical seal drive (magnetic coupling for cells). The drive shaft has a simple and secure connection for the motor. The top plate is equipped with 10mm, PG13.5 and standard, rounded -thread 19mm ports which can be fitted with a septum and retaining collar to allow flaming of the port during inoculation etc. (number and type varies according to specific fermenter). Bottom ports are 25mm Ingold standard and numbers vary by size.
The vessels are sterilized in situ (Techfors and Techfors S) with automatic sequencing. Reagent bottles and inlet pipes etc are usually autoclaved separately (options & alternatives, depending on size and configuration)..
Stirring
The fermenter has a powerful brushless motor with integral speed controller for speeds from 100 - 1500 rpm, depending on the vessel size and number of impellors. Powerful fluid cooled motors are standard for Techfors.
x-DDC Control System:
All fermenter models except Minifors incorporate the x-DDC (eXtended DDC microprocessor control) which uses a high-performance digital system. With the use of almost identical instrumentation, the step from Bench-scale to larger equipment is made considerably easier.
The control panel is compact and is usually mounted above the base unit for clear visibility. The panel can be removed if required. Programming of parameters is via a splashproof keypad with a rotary knob for navigation and an LCD display. Communication is via a robust data-cable. For each fermenter, process information such as parameter set points, PID values etc are stored automatically on an interchangeable "memory card" . This allows changes in fermentation processes by fitting an alternative card or downloading a recipe without re-programming and the chance of errors plus an unlimited number of recipes to be stored.
An "open frame" gas supply system with four magnetic valves allow addition of components for automatic mixing of gasses for oxygen supplementation. An optional mass flow control valve ensures a controlled, steady flow of gas into the vessel. Agitation & temperature control. Four high-accuracy digital peristaltic pumps supplied as standard with the option to add an integral analogue feed pump. Addition and removal of liquids is therefore possible with a standard system (using the antifoam pump for medium take-off). Accepts a conductive probe for automatic control of antifoam addition or media addition/removal in a vessel. Works together with the integral peristaltic pumps.
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振荡在生物培养中的作用
生物培养分为静态培养和振荡培养。振荡培养,亦称悬浮培养,是指把微生物细胞接种于液体培养基中,并放置在摇床或振荡器上不停振荡的一种培养方法。广泛应用于菌种筛选和微生物扩大培养,是微生物生理、生化、发酵和其他生命科学研究领域中常用的培养方式。振荡培养不适用于含有易挥发性化学溶剂,低浓度爆炸气体和低燃点气体的物质以及有毒物质的培养。
生物产业
2023/07/28
箱体分舱段,培养条件更稳定
二氧化碳培养箱是通过在培养箱箱体内模拟形成一个类似细胞/组织在生物体内的生长环境,培养箱要求稳定的温度(比如哺乳动物为37°C)、稳定的CO2水平(一般为5-7%)、恒定的酸碱值(pH值一般为7.2-7.4)、较高的湿度,是细胞、组织、细菌培养的一种仪器,是开展免疫学、肿瘤学、遗传学及生物工程所必须的关键设备。
生物产业
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为什么细胞培养需要二氧化碳?
CO2既是细胞代谢产物,也是细胞所需成分,它主要与维持培养液的pH有直接关系。动物细胞多数需要微碱性环境,pH为7.2~7.4,以不超出6.8~7.6为宜。在细胞培养过程中,随着CO2释放量的增多,培养基会变酸,因此常加入NaHCO3(与CO2溶于水后所形成的H2CO3构成一个缓冲对)来调节pH,以保持培养液中溶解的二氧化碳的浓度。同时细胞培养的器皿需要一定程度的透气,以便于气体的交换。
生物产业
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